ID T1JTH5_TETUR Unreviewed; 515 AA.
AC T1JTH5;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Carboxylesterase type B domain-containing protein {ECO:0000259|Pfam:PF00135};
OS Tetranychus urticae (Two-spotted spider mite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae;
OC Tetranychoidea; Tetranychidae; Tetranychus.
OX NCBI_TaxID=32264 {ECO:0000313|EnsemblMetazoa:tetur01g14170.1, ECO:0000313|Proteomes:UP000015104};
RN [1] {ECO:0000313|Proteomes:UP000015104}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=London {ECO:0000313|Proteomes:UP000015104};
RA Rombauts S.;
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:tetur01g14170.1}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetylcholine + H2O = acetate + choline + H(+);
CC Xref=Rhea:RHEA:17561, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; EC=3.1.1.7;
CC Evidence={ECO:0000256|ARBA:ARBA00000347};
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000256|ARBA:ARBA00005964}.
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DR EMBL; CAEY01000481; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; T1JTH5; -.
DR SMR; T1JTH5; -.
DR ESTHER; tetur-t1jth5; Cholinesterase-like.
DR EnsemblMetazoa; tetur01g14170.1; tetur01g14170.1; tetur01g14170.
DR eggNOG; KOG4389; Eukaryota.
DR HOGENOM; CLU_006586_13_0_1; -.
DR OMA; HPENDRQ; -.
DR Proteomes; UP000015104; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003990; F:acetylcholinesterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR000997; Cholinesterase.
DR PANTHER; PTHR43918; ACETYLCHOLINESTERASE; 1.
DR PANTHER; PTHR43918:SF4; CARBOXYLIC ESTER HYDROLASE; 1.
DR Pfam; PF00135; COesterase; 1.
DR PRINTS; PR00878; CHOLNESTRASE.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022487};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000015104};
KW Serine esterase {ECO:0000256|ARBA:ARBA00022487};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 9..30
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 37..501
FT /note="Carboxylesterase type B"
FT /evidence="ECO:0000259|Pfam:PF00135"
FT ACT_SITE 226
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
FT ACT_SITE 341
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
FT ACT_SITE 461
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR600997-1"
SQ SEQUENCE 515 AA; 57257 MW; BA2D418C1EF3C73B CRC64;
MKCELNSPIY LLMSVILLTA MPFVLLHFIY GSAVTDDAQL TISTGSLKGK VVTFRGIKVY
QFLGIPFAEP PIGDLRFKKP LPVKSWNKLL NVDRWGPHCP QINFQWSEDC LYLNVFTSFS
ALNVAKNIES TNQSRSVMVW FYGGAFISGS ANMPDLYDGT MLAALNDVII VTVNYRIGPL
GFLYLPEYGI PGNMGLWDQQ LALQWVQDNI QYFDGDPGKV TIFGESAGSR SVSAHMVSPQ
LVGLFNKAIM QSGVLSDSNN TLTRHASSLV FLDKLNCSQS TATDVQSCLA AYKFGVVSEA
DSVSVGVVVG DEFIPDSIET AISKQKLDPN INILLGTVGY EGAFSLASTI DPQMFDPINP
LNLTALDVRN ILRKWFKQFS TDSLMQIYFA DVDPNDSDKL RQILSKAVGD VQIACPAYSL
GRKLVKDNKQ SAVYAYYQSQ KSITKPIYLF PSGSTWFPAT HTNDLPFVFG YPFAVNETYD
KEEVILSSIM MQTWTNFAKH GYVRDCMLRK IHRSN
//
