ID   T1KZW3_TETUR            Unreviewed;       954 AA.
AC   T1KZW3;
DT   16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT   16-OCT-2013, sequence version 1.
DT   27-MAR-2024, entry version 47.
DE   RecName: Full=alpha-1,2-Mannosidase {ECO:0000256|RuleBase:RU361193};
DE            EC=3.2.1.- {ECO:0000256|RuleBase:RU361193};
OS   Tetranychus urticae (Two-spotted spider mite).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Acariformes; Trombidiformes; Prostigmata; Eleutherengona; Raphignathae;
OC   Tetranychoidea; Tetranychidae; Tetranychus.
OX   NCBI_TaxID=32264 {ECO:0000313|EnsemblMetazoa:tetur29g00310.1, ECO:0000313|Proteomes:UP000015104};
RN   [1] {ECO:0000313|Proteomes:UP000015104}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=London {ECO:0000313|Proteomes:UP000015104};
RA   Rombauts S.;
RL   Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EnsemblMetazoa:tetur29g00310.1}
RP   IDENTIFICATION.
RG   EnsemblMetazoa;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR601382-2};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 47 family.
CC       {ECO:0000256|ARBA:ARBA00007658, ECO:0000256|RuleBase:RU361193}.
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DR   EMBL; CAEY01000758; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; T1KZW3; -.
DR   STRING; 32264.T1KZW3; -.
DR   EnsemblMetazoa; tetur29g00310.1; tetur29g00310.1; tetur29g00310.
DR   eggNOG; KOG2430; Eukaryota.
DR   HOGENOM; CLU_003818_4_0_1; -.
DR   OMA; NYVCANP; -.
DR   Proteomes; UP000015104; Unassembled WGS sequence.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004571; F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 3.50.30.30; -; 1.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR044674; EDEM1/2/3.
DR   InterPro; IPR001382; Glyco_hydro_47.
DR   InterPro; IPR046450; PA_dom_sf.
DR   InterPro; IPR003137; PA_domain.
DR   InterPro; IPR036026; Seven-hairpin_glycosidases.
DR   PANTHER; PTHR45679; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2; 1.
DR   PANTHER; PTHR45679:SF2; ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3; 1.
DR   Pfam; PF01532; Glyco_hydro_47; 1.
DR   Pfam; PF02225; PA; 1.
DR   PRINTS; PR00747; GLYHDRLASE47.
DR   SUPFAM; SSF52025; PA domain; 1.
DR   SUPFAM; SSF48225; Seven-hairpin glycosidases; 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR601382-2};
KW   Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW   Glycosidase {ECO:0000256|RuleBase:RU361193};
KW   Hydrolase {ECO:0000256|RuleBase:RU361193};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR601382-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000015104}.
FT   DOMAIN          694..778
FT                   /note="PA"
FT                   /evidence="ECO:0000259|Pfam:PF02225"
FT   REGION          800..827
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        109
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT   ACT_SITE        255
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT   ACT_SITE        350
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT   ACT_SITE        369
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601382-1"
FT   BINDING         455
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601382-2"
SQ   SEQUENCE   954 AA;  108085 MW;  F3807FE05422EE73 CRC64;
     MCLIQCMSNE EIQSLRDEVK EMFYHGYNSY MEKAYPADEL MPLSCRGRYR DREPNRGDID
     DALGNFSLTL VDTLDTLVIF NNISEFERGV ALLVKDLNFD TDVVVSVFET NIRVLGGLLS
     GHIFSNYIKD RWKDHLQWYK NELLLLAQDL GYRLLPAFNT STGIPHPRIN LKFGMRSPQI
     INVRETCTSC AGTMILEFAA LSRLTGEPIF EIAARKAMDY LWNQRHRSSD LVGTVLNIHN
     GDWVRRDSGV GAGIDSYYEY LLKGYILLGD ETYLQRFNQH YKGIMKYVSQ GPLLVDVHMH
     RPKSNAKNFM DSLLAFWPGL QVLKGDIKPA IATHEMLYQV AQRHNFLIPE AFSINDFEAH
     WSGYFLRPEF IESTYFLFKA TGDHHYLEVG KQVMEGLQKY TRTECGFAAV KDVRTRTKED
     RMDSFFLTET LKYLYLLFAT KKDLIVDLDQ FVFTTEAHFL PLSLSSMQLD EISKISEKAD
     ALSPPPSSFL TFVDEEKDGS MQYFRHCPNV KYLIKKDLKV DYVRDIRETL KGYIANSRDA
     SLKYARSSSQ STSTPSLNLA SSTCSMSYND HGDLSEKRLS LQPQDFSATN KEHLEIIKKM
     GINVGFTTDG RIQLIHSAAT AENVDKGVEG ALFMQEMLRL SKQQVNQFNS KHQWLSFISP
     STGSMLKFKS GIAQFGLQLK QPLSGKVSIA DPIKACSELL PESNSAISGK IAIVERGECT
     FVQKARVVQK AGAIGCIVID DVIGTTEETL PMFAMSGDGK DDVSIPVTFL YYQQGHSLMW
     FLKQKDDLVI EINAADSKDN NLDFSSSKPS EPSNNEDKDG SYEDDSEVMS PVEAYAEITD
     ITQQIIGGNG EDTERGMKVV EAVLNSFHHD LTLKYQQYVK NNFNSIVEGT ILQDMFSSLQ
     SSLKNMKTVF ESEGEPLLAR SKRKRNLMSL RTCNTLTYEH LISTYTICPR VNTD
//