ID T5A3M6_OPHSC Unreviewed; 1085 AA.
AC T5A3M6;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Regulator of nonsense transcripts 1 {ECO:0000313|EMBL:EQL00060.1};
GN ORFNames=OCS_04232 {ECO:0000313|EMBL:EQL00060.1};
OS Ophiocordyceps sinensis (strain Co18 / CGMCC 3.14243) (Yarsagumba
OS caterpillar fungus) (Hirsutella sinensis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Ophiocordyceps.
OX NCBI_TaxID=911162 {ECO:0000313|EMBL:EQL00060.1, ECO:0000313|Proteomes:UP000019374};
RN [1] {ECO:0000313|EMBL:EQL00060.1, ECO:0000313|Proteomes:UP000019374}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Co18 / CGMCC 3.14243 {ECO:0000313|Proteomes:UP000019374};
RC TISSUE=Fruit-body {ECO:0000313|EMBL:EQL00060.1};
RX DOI=10.1007/s11434-013-5929-5;
RA Hu X., Zhang Y., Xiao G., Zheng P., Xia Y., Zhang X., St Leger R.J.,
RA Liu X., Wang C.;
RT "Genome survey uncovers the secrets of sex and lifestyle in caterpillar
RT fungus.";
RL Chin. Sci. Bull. 58:2846-2854(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13066;
CC Evidence={ECO:0000256|ARBA:ARBA00000600};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
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DR EMBL; KE652976; EQL00060.1; -; Genomic_DNA.
DR AlphaFoldDB; T5A3M6; -.
DR eggNOG; KOG1802; Eukaryota.
DR HOGENOM; CLU_001666_4_0_1; -.
DR Proteomes; UP000019374; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:InterPro.
DR CDD; cd21407; 1B_UPF1-like; 1.
DR CDD; cd18039; DEXXQc_UPF1; 1.
DR CDD; cd18808; SF1_C_Upf1; 1.
DR CDD; cd21400; ZBD_UPF1-like; 1.
DR Gene3D; 2.40.30.230; -; 1.
DR Gene3D; 6.10.140.1240; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR045055; DNA2/NAM7-like.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047187; SF1_C_Upf1.
DR InterPro; IPR040812; UPF1_1B_dom.
DR InterPro; IPR018999; UPF1_CH/ZBD.
DR PANTHER; PTHR10887; DNA2/NAM7 HELICASE FAMILY; 1.
DR PANTHER; PTHR10887:SF364; REGULATOR OF NONSENSE TRANSCRIPTS 1; 1.
DR Pfam; PF13086; AAA_11; 1.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF04851; ResIII; 1.
DR Pfam; PF18141; UPF1_1B_dom; 1.
DR Pfam; PF09416; UPF1_Zn_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51997; UPF1_CH_RICH; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022806};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|PROSITE-
KW ProRule:PRU01341}; Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000019374};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU01341};
KW Zinc-finger {ECO:0000256|PROSITE-ProRule:PRU01341}.
FT DOMAIN 87..244
FT /note="Upf1"
FT /evidence="ECO:0000259|PROSITE:PS51997"
FT REGION 42..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 95..127
FT /note="C3H"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
FT REGION 109..137
FT /note="CC/SHH/C"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
FT REGION 155..185
FT /note="C4"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01341"
SQ SEQUENCE 1085 AA; 119737 MW; 67DB1ED4B548A93C CRC64;
MEGAFTHVGN HLISDSAAAI NAGADDLSAL DPDESLLYGK FPATSGRRRA DDDDNQTEAF
EEDDNDSLNS VPVDGMIGMK LKNSDEEKQL PAHACAYCGL HSPASVVKCL TCNKWFCGAR
GNGSSTHIVN HLVRARHKEV QLHPESTLGD TVLECYNCGT KNAFLLGFIP AKSDTVVVLL
CRQPCAASTS NKDMNWDISR WEPLIEERAF LGWLVSPPSD SEQLRSRHVS ANTISRLEEM
WKVDPNATAV DLDKAANIDD DPDPVLLRYE DPYHYQNIFG PLVKMESDYD KKLKEAQSED
GLTVRWDYGL NNKHLVSFNL HKIESGDVKL AVGDEMRLRY NGELRDPWEG VGYVIKIPNS
QSDEVCLELR KNGNDKSVPT DLSHNFSADY VWKATSYDRM QLAMKTFAVD ELSVSGYIYY
VLLGHEVQLQ PMKANQPKKW SAPGLPDLNQ SQVDAIKSVL QKPISLIQGP PGTGKTVTSA
TIIFHLAKTT GNQVLVCAPS NVAVDQLCER VHRTGLKVVR LTAKSREDVE SSVSFLALHE
QVRMAEHNTE LVKLSQLKTD VGELSSQDEK KFKQLTKLAE REILQNADVV CCTCVGAGDP
RLSKMKFRNV LIDESTQSAE PECLIPLILG CKQVVLVGDH KQLGPVIMNK KAAKAGLNQS
LFERLIHLQL SPIRLNTQYR MHPCLSEFPS NMFYEGALQN GITHEQRLRK DVDFPWPVAE
MPMMFWSNLG GEEISTSGTS YLNRTEASNV EKTVTRFFRA GVKPSEIGVI TPYEGQRSYI
VTTMQNSGQY KKELYKEVEV ASVDAFQGRE KDFIVLSCVR SNENQGIGFL SDPRRLNVAL
TRAKYGLVIL GNPKVLSKHE LWHNLLVHFK DRKCFVEGPL TNLQACLLQF SRPKVSYRQK
NNYQSQYMAS GYANGRHNGG PPGRDVDAAS MMSYIPDDVS SIQGSAFGGA ALNTAFPPMF
SSFTPEHWPG LPGVGGPGRG NKTRGRATES VAGESVANSE LMETTATTSI IGGKGVGQGG
VSLGAGLHDA VTGLRPVSYT QSDRLKHYVE SNGRMAHSSG FARRYDDDEK SIGTAFHSQV
GGGYD
//
