ID TAF2_ARATH Reviewed; 1390 AA.
AC Q8LPF0; F4HTS1; Q6SJQ9; Q9C9B7;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 24-JAN-2024, entry version 129.
DE RecName: Full=Transcription initiation factor TFIID subunit 2;
DE AltName: Full=TBP-associated factor 2;
DE Short=AtTAF2;
GN Name=TAF2; OrderedLocusNames=At1g73960; ORFNames=F2P9.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, NOMENCLATURE, AND TISSUE
RP SPECIFICITY.
RX PubMed=15527982; DOI=10.1016/j.gene.2004.08.023;
RA Lago C., Clerici E., Mizzi L., Colombo L., Kater M.M.;
RT "TBP-associated factors in Arabidopsis.";
RL Gene 342:231-241(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=17340043; DOI=10.1007/s11103-007-9135-1;
RA Lawit S.J., O'Grady K., Gurley W.B., Czarnecka-Verner E.;
RT "Yeast two-hybrid map of Arabidopsis TFIID.";
RL Plant Mol. Biol. 64:73-87(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: TAFs are components of the transcription factor IID (TFIID)
CC complex that is essential for mediating regulation of RNA polymerase
CC transcription. {ECO:0000250}.
CC -!- SUBUNIT: Component of the TFIID complex. TFIID is composed of TATA
CC binding protein (TBP) and a number of TBP-associated factors (TAFs)
CC whose MWs range from 14-217 kDa.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LPF0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LPF0-2; Sequence=VSP_053299;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves and inflorescences.
CC {ECO:0000269|PubMed:15527982}.
CC -!- SIMILARITY: Belongs to the TAF2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52531.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY457045; AAR21621.1; -; mRNA.
DR EMBL; AC016662; AAG52531.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35530.1; -; Genomic_DNA.
DR EMBL; AY102124; AAM26691.1; -; mRNA.
DR PIR; C96767; C96767.
DR RefSeq; NP_177536.2; NM_106055.5. [Q8LPF0-1]
DR AlphaFoldDB; Q8LPF0; -.
DR SMR; Q8LPF0; -.
DR BioGRID; 28952; 4.
DR IntAct; Q8LPF0; 2.
DR STRING; 3702.Q8LPF0; -.
DR iPTMnet; Q8LPF0; -.
DR PaxDb; 3702-AT1G73960-1; -.
DR ProteomicsDB; 233008; -. [Q8LPF0-1]
DR EnsemblPlants; AT1G73960.1; AT1G73960.1; AT1G73960. [Q8LPF0-1]
DR GeneID; 843733; -.
DR Gramene; AT1G73960.1; AT1G73960.1; AT1G73960. [Q8LPF0-1]
DR KEGG; ath:AT1G73960; -.
DR Araport; AT1G73960; -.
DR TAIR; AT1G73960; TAF2.
DR eggNOG; KOG1932; Eukaryota.
DR HOGENOM; CLU_005057_0_0_1; -.
DR InParanoid; Q8LPF0; -.
DR OrthoDB; 11119at2759; -.
DR PhylomeDB; Q8LPF0; -.
DR PRO; PR:Q8LPF0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LPF0; baseline and differential.
DR Genevisible; Q8LPF0; AT.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0005669; C:transcription factor TFIID complex; IEA:InterPro.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd09839; M1_like_TAF2; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 1.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR InterPro; IPR037813; TAF2.
DR PANTHER; PTHR15137; TRANSCRIPTION INITIATION FACTOR TFIID; 1.
DR PANTHER; PTHR15137:SF9; TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Coiled coil; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1390
FT /note="Transcription initiation factor TFIID subunit 2"
FT /id="PRO_0000424039"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1104..1132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1240..1377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1286..1333
FT /evidence="ECO:0000255"
FT COMPBIAS 1104..1121
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1240..1261
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1285..1313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1339..1357
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 910..929
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17340043"
FT /id="VSP_053299"
FT CONFLICT 1350
FT /note="E -> D (in Ref. 2; AAR21621)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1390 AA; 155748 MW; 718EE64B69F0FF47 CRC64;
MAKARKPKNE EAPGAKTSEN TGAKVLHQKL FLSIDFKKRQ IYGYTELEVS VPDIGIVGLH
AENLGIESVL VDGEPTVFEY YPHHQNSETE SNWNSVSDPA SAADAAAMEY VGVLKREDTA
NLLINCCKPS KDLSEQLDSV TLENGSQSSG EAKQNVKLIR INYWVEKIES GIHFDGNIVH
TDNQMRRARC WFPCIDDEYH RCSFDLEFTV PHNFVAVSVG KLLYQVMCKE DTTQKTYVYE
LAIPIAPRWV SLVAGPLEIL PDQTNFLISN LCLPHDLSRL RNTMEFFHEA YSYYEDYLSA
NFPFGFYKQV FLPPEMVVTS STSGASLSIF SSHILYDERV IDQTIDTRIK LASALAKQWF
GVYITPESPN DDWLLDGLAG FLTDMFIKQF LGNNEARYRR YKANCAVCKA DDSGAMCLSS
SPSCRDLFGT HSIGMHGKIR SWKSGAVLQM LEKQMGSDSF RKILQKIISR AKDPSNSIRS
LSTKEFRQFA NKIGNLERPF LKEFFQRWVA SYGCPVLRIG LSYNKRKNNV EMAALRECTA
ALDARLSVIG ATSDSESRDV DAGWPGIMSI RVYELDGMSD HPKLPMAGDR WQLLELPCHS
KLAAKRYQKP KKGGKPDGAE DNVDAIAPLE NKTSIESPLA WIKADPEMEY IAEIHLHQPL
QMWVNQLEKD GDVVAQAQAI ASLEALKQHS FSIVNALKNV LTDSKVFWRI RIAAAFALAK
TASEESDWAG LQHLIKFYKS RRFDAEIGLP KPNDFRDFPE YFVLEAIPHA IAIVRGAEGK
SPREAVEFIL QLLKYNDNSG NSYSDVFWLA VLVQSVGDLE FCQQSLTFLA PLLKRIDRLL
QFDRLMPSYN GILTISCIRT LAQTALKLSD SISFDHICKL IEPFRNSDTI LQIRIEGSRA
LLDIEYQSKG ISSALLLFMK YLVEESSLRG QVKLCVHTMR LCQIAVGCDS DDCVDTVTLL
DLLHLFKSHV VFNNELLRYY LFCIFQILAG RPPTLFGVPK EKPLQLVDVE ACIEPKNVFL
VPGAEAGEPS LSALGDAKGQ SLDVAPYGVP IIPQEMFMPI VPELMLPEPV AAYDETQHLE
PRMESQNQPS HENPIVHEIP SDVEGPTEEL AHREANPPTK EPQKEPDVVS VSVSHEVKKS
VIRIKVRPSG ATSRAEGSAR TIERSQGIVV RHDIDRGQTS SASVDAPQRI STDAVSISNQ
NHVEEVNSCH DVGSRMTASI GSVKFASEGD IFGKELQCTA ESGKPSTSQK ADNNNRTVPP
SFLPLDHSME NEAQQKYASL QTLSIGKEKE KKKDKEKKEK KRKREDPVYL EKKRLKKEKK
RKEKEMAKLV SSTTDPAKKK IESVAEVKEE EPSDGAMLIK VEPKTEPSTA EARPLPKFRI
KLKSKAFNNS
//
