ID TFE_METTP Reviewed; 167 AA.
AC A0B9P3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 1.
DT 27-MAR-2024, entry version 95.
DE RecName: Full=Transcription factor E {ECO:0000255|HAMAP-Rule:MF_01909};
DE Short=TFE {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=TFIIE subunit alpha homolog {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=Transcription initiation factor TFIIE {ECO:0000255|HAMAP-Rule:MF_01909};
GN Name=tfe {ECO:0000255|HAMAP-Rule:MF_01909}; OrderedLocusNames=Mthe_1650;
OS Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 /
OS PT) (Methanosaeta thermophila).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanotrichales; Methanotrichaceae; Methanothrix.
OX NCBI_TaxID=349307;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6194 / JCM 14653 / NBRC 101360 / PT;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Chain P.,
RA Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Smith K.S., Ingram-Smith C., Richardson P.;
RT "Complete sequence of Methanosaeta thermophila PT.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor that plays a role in the activation of
CC archaeal genes transcribed by RNA polymerase. Facilitates transcription
CC initiation by enhancing TATA-box recognition by TATA-box-binding
CC protein (Tbp), and transcription factor B (Tfb) and RNA polymerase
CC recruitment. Not absolutely required for transcription in vitro, but
CC particularly important in cases where Tbp or Tfb function is not
CC optimal. It dynamically alters the nucleic acid-binding properties of
CC RNA polymerases by stabilizing the initiation complex and destabilizing
CC elongation complexes. Seems to translocate with the RNA polymerase
CC following initiation and acts by binding to the non template strand of
CC the transcription bubble in elongation complexes. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
CC -!- SUBUNIT: Monomer. Interaction with RNA polymerase subunits RpoF and
CC RpoE is necessary for Tfe stimulatory transcription activity. Able to
CC interact with Tbp and RNA polymerase in the absence of DNA promoter.
CC Interacts both with the preinitiation and elongation complexes.
CC {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- DOMAIN: The winged helix domain is involved in binding to DNA in the
CC preinitiation complex. {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- SIMILARITY: Belongs to the TFE family. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
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DR EMBL; CP000477; ABK15417.1; -; Genomic_DNA.
DR AlphaFoldDB; A0B9P3; -.
DR SMR; A0B9P3; -.
DR STRING; 349307.Mthe_1650; -.
DR KEGG; mtp:Mthe_1650; -.
DR HOGENOM; CLU_100097_0_0_2; -.
DR OrthoDB; 5935at2157; -.
DR Proteomes; UP000000674; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation at RNA polymerase II promoter; IEA:InterPro.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR HAMAP; MF_01909; TFE_arch; 1.
DR InterPro; IPR016481; TF_E_archaea.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00373; transcription factor E; 1.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR PIRSF; PIRSF006373; TF_E_archaea; 1.
DR SMART; SM00531; TFIIE; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 3: Inferred from homology;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..167
FT /note="Transcription factor E"
FT /id="PRO_0000326607"
FT DOMAIN 5..87
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01909"
SQ SEQUENCE 167 AA; 19306 MW; 98ECAFA200F368B6 CRC64;
MVAVENPIHR AYLLKIVGEE GLRIVEAMPE EEVTDEHLAE ITGISLNTVR KSLYLLYEHR
LAIYRRKRDP ESGWLTYLWK ICPESLDSAL EAEVRKLINK LNARLRYEKD HVFYACVNGC
ARFVFEEASE NNFTCPFCQG SLEYMENSTI VEVIEERIKE LSAALCS
//
