ID TGB1_WCMVO Reviewed; 236 AA.
AC P15403;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 27-MAR-2024, entry version 108.
DE RecName: Full=Movement and silencing protein TGBp1;
DE AltName: Full=25 kDa protein;
DE AltName: Full=Silencing suppressor P25;
DE AltName: Full=Triple gene block 1 protein;
DE Short=TGBp1;
GN ORFNames=ORF2;
OS White clover mosaic virus (strain O) (WCMV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Alphaflexiviridae; Potexvirus; White clover mosaic virus.
OX NCBI_TaxID=12190;
OH NCBI_TaxID=3898; Trifolium.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2353451; DOI=10.1016/0042-6822(90)90469-8;
RA Beck D.L., Forster R.L.S., Bevan M.W., Boxen K.A., Lowe S.C., Gardner R.C.;
RT "Infectious transcripts and nucleotide sequence of cloned cDNA of the
RT potexvirus white clover mosaic virus.";
RL Virology 177:152-158(1990).
RN [2]
RP REVIEW.
RX PubMed=15828680; DOI=10.1094/mpmi-18-0283;
RA Verchot-Lubicz J.;
RT "A new cell-to-cell transport model for Potexviruses.";
RL Mol. Plant Microbe Interact. 18:283-290(2005).
CC -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC through plasmosdesmata, without any budding. The movement protein
CC allows efficient cell to cell propagation, by bypassing the host cell
CC wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC suppressor of RNA-mediated gene silencing, also known as post-
CC transcriptional gene silencing (PTGS), a mechanism of plant viral
CC defense that limits the accumulation of viral RNAs (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC Interacts with host AGO1; this interaction targets the host protein for
CC degradation, thereby suppressing the antiviral RNA silencing (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC cell propagation. TGBp1 is the main movement protein that physically
CC cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC facilitate TGBp1 function.
CC -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC {ECO:0000305}.
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DR EMBL; X16636; CAA34629.1; -; Genomic_RNA.
DR PIR; B46350; B46350.
DR SMR; P15403; -.
DR Proteomes; UP000007628; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01443; Viral_helicase1; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 3: Inferred from homology;
KW Host cytoplasm; Host-virus interaction;
KW Inhibition of host innate immune response by virus; RNA-binding;
KW Suppressor of RNA silencing; Transport; Viral immunoevasion;
KW Viral movement protein.
FT CHAIN 1..236
FT /note="Movement and silencing protein TGBp1"
FT /id="PRO_0000222576"
FT DOMAIN 1..117
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 118..236
FT /note="(+)RNA virus helicase C-terminal"
SQ SEQUENCE 236 AA; 26451 MW; D470D75B46E9D418 CRC64;
MDHIHHLLSS HGFTRTRLAK SKPIVVHAIA GSGKSTVIRK ILSDLPNARA YTLGKPDPYS
LSNPTIKAFA QFKRGTLDIL DEYGQLPFAD LDSSFEFIFT DPYQAPTDNL FEPHYTLEIT
YRFGPNTCNL LNQAFQSNIT SLVTQDNISF GSPYLVDPVG TILAFQPDTY LILCLHQAPF
FKVSEVIGYQ WPTVTLYLAC KISEIPEEER HLLFIGLTRH TESLLILGPD AFDSSP
//
