ID TIM50_SCHPO Reviewed; 452 AA.
AC O13636; Q7LL08;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 126.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim50;
DE Flags: Precursor;
GN Name=tim50; ORFNames=pi044, SPBC17A3.01c, SPBC8D2.21c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=10620777;
RX DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT yeast chromosome II: sequence analysis and characterization of the genomic
RT DNA and cDNAs encoded on the segment.";
RL Yeast 16:71-80(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane. Required to direct preproteins
CC in transit and direct them to the channel protein TIM23, and possibly
CC facilitates transfer of the translocating proteins from the TOM complex
CC to the TIM23 complex (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM23 complex, at least composed of tim23,
CC tim17, tim50 and tim21. Interacts with preproteins in transit (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR EMBL; AB004537; BAA21424.1; -; Genomic_DNA.
DR EMBL; CU329671; CAA17836.2; -; Genomic_DNA.
DR PIR; T39693; T39693.
DR RefSeq; NP_595583.2; NM_001021478.3.
DR AlphaFoldDB; O13636; -.
DR SMR; O13636; -.
DR ComplexPortal; CPX-540; Mitochondrial inner membrane pre-sequence translocase complex.
DR STRING; 284812.O13636; -.
DR MaxQB; O13636; -.
DR PaxDb; 4896-SPBC17A3-01c-1; -.
DR EnsemblFungi; SPBC17A3.01c.1; SPBC17A3.01c.1:pep; SPBC17A3.01c.
DR GeneID; 2539802; -.
DR KEGG; spo:SPBC17A3.01c; -.
DR PomBase; SPBC17A3.01c; tim50.
DR VEuPathDB; FungiDB:SPBC17A3.01c; -.
DR eggNOG; KOG2832; Eukaryota.
DR HOGENOM; CLU_023309_1_0_1; -.
DR InParanoid; O13636; -.
DR OMA; DTHAPHA; -.
DR PhylomeDB; O13636; -.
DR PRO; PR:O13636; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR GO; GO:0016887; F:ATP hydrolysis activity; IC:PomBase.
DR GO; GO:0006886; P:intracellular protein transport; NAS:ComplexPortal.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; ISS:PomBase.
DR CDD; cd07521; HAD_FCP1-like; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR004274; FCP1_dom.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR PANTHER; PTHR12210; DULLARD PROTEIN PHOSPHATASE; 1.
DR PANTHER; PTHR12210:SF3; MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM50; 1.
DR Pfam; PF03031; NIF; 1.
DR SMART; SM00577; CPDc; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
DR PROSITE; PS50969; FCP1; 1.
PE 3: Inferred from homology;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..27
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 28..452
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim50"
FT /id="PRO_0000043136"
FT TOPO_DOM 28..99
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..452
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT DOMAIN 172..316
FT /note="FCP1 homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT REGION 30..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 433..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 452 AA; 52572 MW; 83E516CBED6271B3 CRC64;
MLNRSLLFRN LRLARPRPFL PLVGKRFVTE KSQSQEEKDT SKITENAKEE VKRDTSSLAK
ESLKLLDLNG LNDESYTGDP GKGPEYTSST MLKREKQARY AFWGFLGLTG GGLLYYGRRY
GPDEKELEKQ YPAAGYSPSD WWNRVKARTN NFFSYYQEPA FEKLLPDPLP EPYNRPYTLV
LSLDDLLIHS EWTRQHGWRT AKRPGLDYFL GYLSMYYEVV IFTRQYLATA KPIIDKIDPY
HVSISAVLTR ESSKYEKGKV IKDLSYLNRD LSRVIMIDTN PESWSKQPDN AIAMAPWTGN
PKDKELVGLI PLLEFIAIMD IKDVRPVLKS YQGKNIPLEY ARREEKLRTK LIEDWNEKKK
KGSSFLFGGR SVSEEPPKLI IDIQRERQKA AYAEFKKYID ENGPKMLEEE KAREAEQKTS
IFNLLFHPEE VQQQQLEQMQ QQQFSPETNA SK
//
