ID TRM44_CAEBR Reviewed; 562 AA.
AC Q60K16; A8WKC3;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 27-MAR-2024, entry version 76.
DE RecName: Full=Probable tRNA (uracil-O(2)-)-methyltransferase;
DE EC=2.1.1.211;
GN ORFNames=CBG24265;
OS Caenorhabditis briggsae.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC (uracil-O(2)-)-methyltransferase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TRM44 family. {ECO:0000305}.
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DR EMBL; HE601461; CAP20917.1; -; Genomic_DNA.
DR RefSeq; XP_002648179.1; XM_002648133.1.
DR AlphaFoldDB; Q60K16; -.
DR STRING; 6238.Q60K16; -.
DR EnsemblMetazoa; CBG24265.1; CBG24265.1; WBGene00042420.
DR GeneID; 8590182; -.
DR KEGG; cbr:CBG_24265; -.
DR CTD; 8590182; -.
DR WormBase; CBG24265; CBP12807; WBGene00042420; -.
DR eggNOG; KOG3790; Eukaryota.
DR HOGENOM; CLU_021025_0_0_1; -.
DR OMA; CFFKLHH; -.
DR OrthoDB; 1353816at2759; -.
DR Proteomes; UP000008549; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016300; F:tRNA (uridine) methyltransferase activity; IBA:GO_Central.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR InterPro; IPR000571; Znf_CCCH.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50103; ZF_C3H1; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Metal-binding; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase; tRNA processing; Zinc; Zinc-finger.
FT CHAIN 1..562
FT /note="Probable tRNA (uracil-O(2)-)-methyltransferase"
FT /id="PRO_0000249897"
FT ZN_FING 535..562
FT /note="C3H1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 520..546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 562 AA; 64910 MW; A5196A7BC8149119 CRC64;
MSPWRCIAEQ LVEYEEPVDA KAYFDKIICL WKEYPNAISN RINTSVPVEE ESQVWNEVIA
NFESKYRFDP ANSSELSIFK LIQRDYKRQS FSNNCYEVAF YEEELLVRFY PIVLDGQQHP
HFESPYSIAC KIPSNSSILL QFFKPVDTPD DHFEFLKTLA FKQLVTWLKS IDLSKTTRKT
HSLLDKESYI STYRHIREDL GRPLVEGWTE NSKPQKVIFE DCGIASYIQE LITSGILPKP
RKFVDIGCGN GLLVHLLNKI GIPGYGVDVR SRKVWKTTLS HVDLRESPVD PQIVVENRPH
FDADVDLLIG NHSDELTPWI PVMAAKLNCN FFLIPCCPYN FFGKYLNNGS HLGPKRMTSQ
YESFFEWTVS VSERLGFDTK MDRLAIPSTK RLCIIGRVPE KGLCPSLEQT IEHMTQGQKF
VARPREIRNN NCMHISVTDR ERIAKKLFDF ILNASDDVRD GWRCGGEVPL AQLAGQLTDD
DKKLMKDQDG GLQTFLRNHH QIFHVFQATA RLRDFRQPVV SRRQQTNPKK QEATNRPKQP
CWMSLNHPDG CPLGPESCRY LH
//
