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Database: UniProt
Entry: TRMD_RHILO
LinkDB: TRMD_RHILO
Original site: TRMD_RHILO 
ID   TRMD_RHILO              Reviewed;         232 AA.
AC   Q98EE1;
DT   06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   26-FEB-2020, entry version 99.
DE   RecName: Full=tRNA (guanine-N(1)-)-methyltransferase;
DE            EC=2.1.1.228;
DE   AltName: Full=M1G-methyltransferase;
DE   AltName: Full=tRNA [GM37] methyltransferase;
GN   Name=trmD; OrderedLocusNames=mll4287;
OS   Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
OS   (Mesorhizobium loti (strain MAFF 303099)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Phyllobacteriaceae; Mesorhizobium.
OX   NCBI_TaxID=266835;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 29417 / CECT 9101 / MAFF 303099;
RX   PubMed=11214968; DOI=10.1093/dnares/7.6.331;
RA   Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y.,
RA   Nakayama S., Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M.,
RA   Tabata S.;
RT   "Complete genome structure of the nitrogen-fixing symbiotic bacterium
RT   Mesorhizobium loti.";
RL   DNA Res. 7:331-338(2000).
CC   -!- FUNCTION: Specifically methylates guanosine-37 in various tRNAs.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC         methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:36899, Rhea:RHEA-COMP:10145, Rhea:RHEA-COMP:10147,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.228;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase TrmD family.
CC       {ECO:0000305}.
DR   EMBL; BA000012; BAB50979.1; -; Genomic_DNA.
DR   RefSeq; WP_010912321.1; NC_002678.2.
DR   SMR; Q98EE1; -.
DR   STRING; 266835.14024375; -.
DR   PRIDE; Q98EE1; -.
DR   EnsemblBacteria; BAB50979; BAB50979; BAB50979.
DR   KEGG; mlo:mll4287; -.
DR   PATRIC; fig|266835.9.peg.3382; -.
DR   eggNOG; ENOG4105D6X; Bacteria.
DR   eggNOG; COG0336; LUCA.
DR   HOGENOM; CLU_047363_0_1_5; -.
DR   KO; K00554; -.
DR   OMA; ILCGHYK; -.
DR   OrthoDB; 525632at2; -.
DR   BioCyc; MLOT266835:G1G20-3390-MONOMER; -.
DR   Proteomes; UP000000552; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052906; F:tRNA (guanine(37)-N(1))-methyltransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1270.20; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   HAMAP; MF_00605; TrmD; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR002649; tRNA_m1G_MeTrfase_bac.
DR   InterPro; IPR023148; tRNA_m1G_MeTrfase_C.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR   PANTHER; PTHR46417; PTHR46417; 1.
DR   Pfam; PF01746; tRNA_m1G_MT; 1.
DR   PIRSF; PIRSF000386; tRNA_mtase; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00088; trmD; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN           1..232
FT                   /note="tRNA (guanine-N(1)-)-methyltransferase"
FT                   /id="PRO_0000060439"
FT   REGION          134..139
FT                   /note="S-adenosyl-L-methionine binding"
FT                   /evidence="ECO:0000250"
FT   BINDING         114
FT                   /note="S-adenosyl-L-methionine; via amide nitrogen"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   232 AA;  25082 MW;  8EBAE40DF7C50167 CRC64;
     MTFKASVLTL YPEMFPGALG LSLAGRALEA GTWSLEAIQI RDFATDKHRT VDDTPAGGGA
     GMVMRADVLA RAIDHASPPG DTRPRLLMSP RGKPLTQARV RELAAGPGAV ILCGRFEGVD
     QRLIEVRGLE EVSIGDFILS GGEPAALVLL DAVVRLLPGV MGNAVSGEEE SFENGLLEHP
     HYTRPQEFEG REIPQVLTSG NHKKIAAWRR EQAEQLTRER RPDLLGAHPL AK
//
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