ID U1GKE0_ENDPU Unreviewed; 1072 AA.
AC U1GKE0;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Pre-mRNA-processing factor 19 {ECO:0000256|RuleBase:RU367101};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU367101};
GN ORFNames=EPUS_06097 {ECO:0000313|EMBL:ERF72341.1};
OS Endocarpon pusillum (strain Z07020 / HMAS-L-300199) (Lichen-forming
OS fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Chaetothyriomycetidae; Verrucariales; Verrucariaceae; Endocarpon.
OX NCBI_TaxID=1263415 {ECO:0000313|EMBL:ERF72341.1, ECO:0000313|Proteomes:UP000019373};
RN [1] {ECO:0000313|Proteomes:UP000019373}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z07020 / HMAS-L-300199 {ECO:0000313|Proteomes:UP000019373};
RX PubMed=24438332; DOI=10.1186/1471-2164-15-34;
RA Wang Y.-Y., Liu B., Zhang X.-Y., Zhou Q.-M., Zhang T., Li H., Yu Y.-F.,
RA Zhang X.-L., Hao X.-Y., Wang M., Wang L., Wei J.-C.;
RT "Genome characteristics reveal the impact of lichenization on lichen-
RT forming fungus Endocarpon pusillum Hedwig (Verrucariales, Ascomycota).";
RL BMC Genomics 15:34-34(2014).
CC -!- FUNCTION: Ubiquitin-protein ligase which is mainly involved pre-mRNA
CC splicing and DNA repair. Required for pre-mRNA splicing as component of
CC the spliceosome. {ECO:0000256|RuleBase:RU367101}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|RuleBase:RU367101};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU367101}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|RuleBase:RU367101}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU367101}.
CC -!- SIMILARITY: Belongs to the WD repeat PRP19 family.
CC {ECO:0000256|ARBA:ARBA00006388, ECO:0000256|RuleBase:RU367101}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KE721110; ERF72341.1; -; Genomic_DNA.
DR RefSeq; XP_007802012.1; XM_007803821.1.
DR AlphaFoldDB; U1GKE0; -.
DR GeneID; 19241044; -.
DR eggNOG; KOG0289; Eukaryota.
DR HOGENOM; CLU_287426_0_0_1; -.
DR OrthoDB; 130592at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000019373; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0000974; C:Prp19 complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IEA:InterPro.
DR GO; GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IEA:InterPro.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; IEA:UniProtKB-UniRule.
DR GO; GO:1902600; P:proton transmembrane transport; IEA:InterPro.
DR CDD; cd16656; RING-Ubox_PRP19; 1.
DR Gene3D; 1.20.1530.20; -; 2.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR024977; Apc4-like_WD40_dom.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR013915; Pre-mRNA_splic_Prp19.
DR InterPro; IPR038959; Prp19.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR43995; PRE-MRNA-PROCESSING FACTOR 19; 1.
DR PANTHER; PTHR43995:SF1; PRE-MRNA-PROCESSING FACTOR 19; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00999; Na_H_Exchanger; 2.
DR Pfam; PF08606; Prp19; 1.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU367101};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU367101};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664,
KW ECO:0000256|RuleBase:RU367101};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187,
KW ECO:0000256|RuleBase:RU367101};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU367101};
KW Reference proteome {ECO:0000313|Proteomes:UP000019373};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728, ECO:0000256|RuleBase:RU367101};
KW Transferase {ECO:0000256|RuleBase:RU367101};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU367101};
KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE-
KW ProRule:PRU00221}.
FT TRANSMEM 12..29
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 36..61
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 67..84
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 96..119
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 125..146
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 243..261
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 380..403
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 471..494
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 595..661
FT /note="U-box"
FT /evidence="ECO:0000259|SMART:SM00504"
FT REPEAT 871..912
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 916..953
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REGION 302..325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1072 AA; 114945 MW; 9D38C036A5A56D08 CRC64;
MSSSTLPYHE PGIVDILIFS SFLLLLNVVN SIIDRYLFCG LIGQILIGIA WGIPGAAWLS
VPLQETIMQL GYLGLLLLVF EGGLSTSWAA LKSNLLLSVA VASTGILMPM VLSFSLIGIA
NATPLQALAA GAALCSTSLG TTFTLLSSSG LVTSRLGVVL SSAAMLDDVV GLVMVQIISN
LGTSSSFRAV TVVRPILVST AFATLVPAVC LFICKPVIHS TSLLRQRSIP KWMQYAPNRS
QRAFLVHVLL LIASVAAANY AGTSNLFTAY LAGAVISWWD SEHAATRQTH NDELNIPVSA
TASVTDRRES RSISPAPAQS PTGINEKKVT DEQHVISEIP TGSKVYIEFF GSAVHRILKP
FFFASIGFSI PISKMFSGSI LWRGILYSIL MMVGKFACSL WILPHREWFL ALRTGKSSAK
HLGIQDLYPS AILGFAMVAR GEIGFLISSI AESEGIFSAD KDSQSSAAPE LFLIVSWAIV
LCTIIGPVCV GLCVRQIQSK MASQGTDRQD EDFDNTWGIW GMLSYASLRS AGNSSRLCGR
FDNAMVMGKE CSKGNYYNTS PALSEFFHWL QRAFSSDALR KYVTPIEPVS IVLRLIKHPV
SGEAPQVPVA SRKSGTIYEK RLIEGYISEN GTEPATGEDL TVDDLIELRT SRTVRPRPPT
LTSIPSLLGV FQEEWDALTL ETYTLKQNLA QTRQELSTAL YQHDAAVRVI ARLTRERDEA
REALSKVSIG SRAAPTNGDA MQVDSSGLPE ALIAKVDETK AMLEKTRRKR PIPEEWATSE
EISTFKPQRT SESLYPGGRI LAINEKDDLA LVGGSDGIAG VYSMSDNRVL HALKGGGGSI
TSGVWAGPKA VISTSAGRVK VFERQEEAAS FSTHAGEATA VALHPSGDII ASVGVDKSYV
LYDLTTSAVA TQVYSNSALV CVQFHPDGHL LAAGGEDGQI KLFDIKTGAQ AATFDLNAAL
KALYFSENGI WLAAVTDSST TISIWDLRKM AEIKSLDTGS GRIDSISWDY TGQFLLTGGP
GGLTVQHYSK ASKAWSEPLK SAVPAVAVAW GKGAHNIAAL DEGGVVTVLG GP
//
