ID U2AYA4_9PSED Unreviewed; 373 AA.
AC U2AYA4;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Multifunctional fusion protein {ECO:0000256|RuleBase:RU363066, ECO:0000256|RuleBase:RU369079};
DE Includes:
DE RecName: Full=Gluconokinase {ECO:0000256|RuleBase:RU363066};
DE EC=2.7.1.12 {ECO:0000256|RuleBase:RU363066};
DE Includes:
DE RecName: Full=TRAP transporter small permease protein {ECO:0000256|RuleBase:RU369079};
GN ORFNames=N878_10805 {ECO:0000313|EMBL:ERI49727.1};
OS Pseudomonas sp. EGD-AK9.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1386078 {ECO:0000313|EMBL:ERI49727.1, ECO:0000313|Proteomes:UP000016488};
RN [1] {ECO:0000313|EMBL:ERI49727.1, ECO:0000313|Proteomes:UP000016488}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EGD-Ak9 {ECO:0000313|Proteomes:UP000016488};
RA Kapley A., Sagarkar S., Bhardwaj P., Qureshi A., Khardenavis A.,
RA Purohit H.J.;
RT "Indian agricultural soil isolates capable of pesticide degradation.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC transport system. {ECO:0000256|RuleBase:RU369079}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-gluconate = 6-phospho-D-gluconate + ADP + H(+);
CC Xref=Rhea:RHEA:19433, ChEBI:CHEBI:15378, ChEBI:CHEBI:18391,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58759, ChEBI:CHEBI:456216;
CC EC=2.7.1.12; Evidence={ECO:0000256|ARBA:ARBA00001329,
CC ECO:0000256|RuleBase:RU363066};
CC -!- PATHWAY: Carbohydrate acid metabolism; D-gluconate degradation.
CC {ECO:0000256|ARBA:ARBA00004875}.
CC -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC protein and the two transmembrane proteins.
CC {ECO:0000256|RuleBase:RU369079}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|RuleBase:RU369079}; Multi-pass membrane protein
CC {ECO:0000256|RuleBase:RU369079}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the TRAP transporter small permease family.
CC {ECO:0000256|RuleBase:RU369079}.
CC -!- SIMILARITY: Belongs to the gluconokinase GntK/GntV family.
CC {ECO:0000256|ARBA:ARBA00008420, ECO:0000256|RuleBase:RU363066}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERI49727.1}.
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DR EMBL; AVOF01000907; ERI49727.1; -; Genomic_DNA.
DR RefSeq; WP_021446291.1; NZ_AVOF01000907.1.
DR AlphaFoldDB; U2AYA4; -.
DR PATRIC; fig|1386078.3.peg.4542; -.
DR OrthoDB; 9795716at2; -.
DR Proteomes; UP000016488; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046316; F:gluconokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd02021; GntK; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR006001; Therm_gnt_kin.
DR InterPro; IPR007387; TRAP_DctQ.
DR NCBIfam; TIGR01313; therm_gnt_kin; 1.
DR PANTHER; PTHR43442; GLUCONOKINASE-RELATED; 1.
DR PANTHER; PTHR43442:SF3; GLUCONOKINASE-RELATED; 1.
DR Pfam; PF13671; AAA_33; 1.
DR Pfam; PF04290; DctQ; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363066};
KW Cell inner membrane {ECO:0000256|RuleBase:RU369079};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU363066};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU369079};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363066};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363066};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU369079};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU369079}; Transport {ECO:0000256|RuleBase:RU369079}.
FT TRANSMEM 222..243
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
FT TRANSMEM 255..273
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
FT TRANSMEM 294..316
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
FT TRANSMEM 328..353
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU369079"
SQ SEQUENCE 373 AA; 40410 MW; 813CBB477480856A CRC64;
MQQQPFALPA RTTLVVMGVS GSGKSEISQA VATTLGWRHI EADHFHPREN VERMRAGIPL
SDDDRSHWLH ALSEQMLAAQ AAGEGFVLAC SALKRAYRER LRAAVPGLQF VHLQIDQATA
LQRVGARPGH FMPTSLVDSQ FATLEAPAGE PQVLTLDAAA SREALLADIC AWLRRDLPEA
GSGAAAAGST TEIDSANHAA QLGAEPIYEG RLARLFDRLT DGLMATLMAF MVLAVFANVV
LRYAFGTGWA GAEELSRLAF VWLVFVGVAS SMRRGELMTF RMIRDRFPQS AQRLLDSLSW
LLVAGASLLA AWGAWMQVQF GWGNQSTVVG YPVVLAMVPV LACMTVLAVL ALVQLINLWR
RAPQASLAHA QVD
//
