ID U2LXH1_9FIRM Unreviewed; 847 AA.
AC U2LXH1;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN ORFNames=RUMCAL_02514 {ECO:0000313|EMBL:ERJ91803.1};
OS Ruminococcus callidus ATCC 27760.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=411473 {ECO:0000313|EMBL:ERJ91803.1, ECO:0000313|Proteomes:UP000016662};
RN [1] {ECO:0000313|EMBL:ERJ91803.1, ECO:0000313|Proteomes:UP000016662}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27760 {ECO:0000313|EMBL:ERJ91803.1,
RC ECO:0000313|Proteomes:UP000016662};
RA Weinstock G., Sodergren E., Wylie T., Fulton L., Fulton R., Fronick C.,
RA O'Laughlin M., Godfrey J., Miner T., Herter B., Appelbaum E., Cordes M.,
RA Lek S., Wollam A., Pepin K.H., Palsikar V.B., Mitreva M., Wilson R.K.;
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERJ91803.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AWVF01000300; ERJ91803.1; -; Genomic_DNA.
DR RefSeq; WP_021680680.1; NZ_KI260297.1.
DR AlphaFoldDB; U2LXH1; -.
DR STRING; 411473.RUMCAL_02514; -.
DR GeneID; 78474313; -.
DR PATRIC; fig|411473.3.peg.2103; -.
DR eggNOG; COG4733; Bacteria.
DR HOGENOM; CLU_008926_0_2_9; -.
DR OrthoDB; 2078139at2; -.
DR Proteomes; UP000016662; Unassembled WGS sequence.
DR GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR CDD; cd14256; Dockerin_I; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 1.10.1330.10; Dockerin domain; 1.
DR Gene3D; 2.60.40.710; Endoglucanase-like; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR001956; CBM3.
DR InterPro; IPR036966; CBM3_sf.
DR InterPro; IPR002105; Dockerin_1_rpt.
DR InterPro; IPR016134; Dockerin_dom.
DR InterPro; IPR036439; Dockerin_dom_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00404; Dockerin_1; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR SUPFAM; SSF63446; Type I dockerin domain; 1.
DR PROSITE; PS51172; CBM3; 1.
DR PROSITE; PS51766; DOCKERIN; 1.
PE 4: Predicted;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..847
FT /note="cellulase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039000301"
FT DOMAIN 538..706
FT /note="CBM3"
FT /evidence="ECO:0000259|PROSITE:PS51172"
FT DOMAIN 776..844
FT /note="Dockerin"
FT /evidence="ECO:0000259|PROSITE:PS51766"
FT REGION 733..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 847 AA; 93880 MW; CDB8E920F4684FD6 CRC64;
MKRFSKGSKR LLSVLLSGAM LAGTCSSVAV GAADEKEANF ARALQYSIYF YDGNMCGTEV
QDNNRYTWRG NCHTYDAQVA MNSTATNLSA DFLTKYKDIL DPDGDGYIDV AGGFHDAGDH
VKFGMPENYS AATLGWGYYE FRDAYQKTGQ DDHIETILRY FNDYLMKCTF LDSNGDVIAH
CYQVGDGDID HAYWNAPELD EMDRPAFFLT GDKPQTDYVA SAAASLAINY LNFKDTDPEY
AQKSLDYATA LYKFAETHEK QLSDNGDGPK AYYNSSKWED DYCWASAWMY KITGDHHYLE
QIFPYYDYYA APCYVYCWND VWGGVQCILG EITSEQYPNF IDEYKKAAGK SPYEEMNCWS
SVAEALNKYM TGGVGTITPA GYFWLNTWGS ARYNAAAQMM ALVYDKYNNN GKPGEYSEWA
KGQMEYLLGD NPMNRAYEVG YDETAAKFPH HRAASGLTKC EDTDEQKHVL YGALVGGPDA
QDKHNDITAD WIYNEVTIDY NAAFVGACAG LYDYYGTDAM EITPDFPPED KNSGSDNGGN
DFWVDAYAVD DIQTSGAGVT KLAIQMRTNS ITPKTDLSMR YYFSIAEMEN KSNISKVTGN
ELYDQASVEA APADGVISGP YQYDASYDPD IYYVEVKWDG YKIANSNKKY QFTVGLYYGD
KWDPTNDWSY QGITKCKDTY QDGSETRTDY ICVYSNGELV GGIEPNGNKP AVTTTTTPAE
TTITTTKTTT VTTKATSTSA ETTTTEQTSS AAPSTDTTET TANTTKTSES GSDVTPSVMY
GDVNLDGRID VTDAVLLNKM AAGAVTGNDQ QRRAGDCNVD GEVDTADSVM LLQFLVHIIQ
YLGPQAK
//
