ID U2M300_9FIRM Unreviewed; 543 AA.
AC U2M300;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Adenine DNA glycosylase {ECO:0000256|ARBA:ARBA00022023};
DE EC=3.2.2.31 {ECO:0000256|ARBA:ARBA00012045};
GN ORFNames=RUMCAL_02228 {ECO:0000313|EMBL:ERJ93713.1};
OS Ruminococcus callidus ATCC 27760.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=411473 {ECO:0000313|EMBL:ERJ93713.1, ECO:0000313|Proteomes:UP000016662};
RN [1] {ECO:0000313|EMBL:ERJ93713.1, ECO:0000313|Proteomes:UP000016662}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27760 {ECO:0000313|EMBL:ERJ93713.1,
RC ECO:0000313|Proteomes:UP000016662};
RA Weinstock G., Sodergren E., Wylie T., Fulton L., Fulton R., Fronick C.,
RA O'Laughlin M., Godfrey J., Miner T., Herter B., Appelbaum E., Cordes M.,
RA Lek S., Wollam A., Pepin K.H., Palsikar V.B., Mitreva M., Wilson R.K.;
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes free adenine bases from 7,8-dihydro-8-
CC oxoguanine:adenine mismatched double-stranded DNA, leaving an
CC apurinic site.; EC=3.2.2.31;
CC Evidence={ECO:0000256|ARBA:ARBA00000843};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the Nth/MutY family.
CC {ECO:0000256|ARBA:ARBA00008343}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERJ93713.1}.
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DR EMBL; AWVF01000276; ERJ93713.1; -; Genomic_DNA.
DR AlphaFoldDB; U2M300; -.
DR STRING; 411473.RUMCAL_02228; -.
DR PATRIC; fig|411473.3.peg.1841; -.
DR eggNOG; COG0494; Bacteria.
DR eggNOG; COG1194; Bacteria.
DR HOGENOM; CLU_037194_0_0_9; -.
DR OrthoDB; 9802365at2; -.
DR Proteomes; UP000016662; Unassembled WGS sequence.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0000701; F:purine-specific mismatch base pair DNA N-glycosylase activity; IEA:UniProtKB-EC.
DR GO; GO:0006284; P:base-excision repair; IEA:InterPro.
DR CDD; cd03431; DNA_Glycosylase_C; 1.
DR CDD; cd00056; ENDO3c; 1.
DR CDD; cd03674; Nudix_Hydrolase_1; 1.
DR Gene3D; 1.10.1670.10; Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal); 1.
DR Gene3D; 3.90.79.10; Nucleoside Triphosphate Pyrophosphohydrolase; 2.
DR InterPro; IPR005760; A/G_AdeGlyc_MutY.
DR InterPro; IPR011257; DNA_glycosylase.
DR InterPro; IPR003265; HhH-GPD_domain.
DR InterPro; IPR023170; HhH_base_excis_C.
DR InterPro; IPR044298; MIG/MutY.
DR InterPro; IPR029119; MutY_C.
DR InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf.
DR InterPro; IPR000086; NUDIX_hydrolase_dom.
DR NCBIfam; TIGR01084; mutY; 1.
DR PANTHER; PTHR42944; ADENINE DNA GLYCOSYLASE; 1.
DR PANTHER; PTHR42944:SF1; ADENINE DNA GLYCOSYLASE; 1.
DR Pfam; PF00730; HhH-GPD; 1.
DR Pfam; PF00293; NUDIX; 1.
DR Pfam; PF14815; NUDIX_4; 1.
DR SMART; SM00478; ENDO3c; 1.
DR SUPFAM; SSF48150; DNA-glycosylase; 1.
DR SUPFAM; SSF55811; Nudix; 2.
DR PROSITE; PS51462; NUDIX; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}.
FT DOMAIN 52..191
FT /note="Nudix hydrolase"
FT /evidence="ECO:0000259|PROSITE:PS51462"
SQ SEQUENCE 543 AA; 62097 MW; B53B36C7799C9A24 CRC64;
MRNVMQEQDV TDWKERLAAY TPETEQERRD RNEILLAAEQ YGTQLLYRSH AESHFTCSGF
VMNPAMDQVL MVYHRIYDSF AWTGGHADGS SDFLWTAVRE AKEETGIQKP YPLTGAILSL
DILPVKAHQK KGVPVPAHQH YNVTYGIIAD QKETLRIQPD ENTAVQWIPV EQLPEICKEP
HMLPVYEKVI HRMRCWKAMQ EQALLQTAQP LLAWYPLHAR DLPWRHTQEP YRIWLSEIML
QQTRVEAVKN YYARFLETFP DIAALAAAEQ DQVNKCWEGL GYYSRAANLR KAAQTIQEQY
GGVFPRTWEE VRGLAGVGDY TAGAVCSICY GLPTPAVDGN VLRVAARVMN HFCEIDRPEQ
KAAVTALLRQ VYLDNPEHCG TLTQAFMEIG ATVCVPNGQP HCEACPLSEL CLGRQYCDAL
RLPQRTAKKA RRQEQYTVFV LQCDGKFALR KRTAKGLLHG LWEYPNVAGI CTTEEAIAQA
SAWHCKPTDL TQTVERKHIF THVEWELYGV YLTCARQDEQ FVWKTAAEIT KEISLPTAFR
QFF
//
