ID U3AEC3_9RHOB Unreviewed; 1154 AA.
AC U3AEC3;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=MBELCI_2081 {ECO:0000313|EMBL:GAD56029.1};
OS Limimaricola cinnabarinus LL-001.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Paracoccaceae; Limimaricola.
OX NCBI_TaxID=1337093 {ECO:0000313|EMBL:GAD56029.1, ECO:0000313|Proteomes:UP000016566};
RN [1] {ECO:0000313|EMBL:GAD56029.1, ECO:0000313|Proteomes:UP000016566}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LL-001 {ECO:0000313|EMBL:GAD56029.1,
RC ECO:0000313|Proteomes:UP000016566};
RA Nishi S., Tsubouchi T., Takaki Y., Koyanagi R., Satoh N., Maruyama T.,
RA Hatada Y.;
RT "Draft Genome Sequence of Loktanella cinnabarina LL-001T, Isolated from
RT Deep-Sea Floor Sediment.";
RL Genome Announc. 1:e00927-13(2013).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAD56029.1}.
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DR EMBL; BATB01000026; GAD56029.1; -; Genomic_DNA.
DR AlphaFoldDB; U3AEC3; -.
DR STRING; 1337093.MBELCI_2081; -.
DR eggNOG; COG1197; Bacteria.
DR Proteomes; UP000016566; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 613..774
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 795..988
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1154 AA; 127299 MW; 75456EF6B24291F3 CRC64;
MVKTNGSRIT VSGAPEGFDA TLVLKEIEKA GGPVIHVARD DKRMAAMRAA LEFYAPDMPV
VTFPAWDCLP YDRVSPAAEI SAARMAVLAG LIHGMPERFV LLTTLNAATQ RVPARATLKD
AAFVAHVGDR MDEAGLRAFL VRMGFTQSPT VTEPGDYAIR GGIIDIYAPG QAAPVRLDMF
GDVLDGARRF DPATQRTTEK LDRIELAPVS EVILDEAAIT RFRQTYRIEF GAAGTDDPLY
EAVTAGRKQS GMEHWLGFFQ DDLETLFDYL PGAPVTLDDQ TAPGRDARWT LIADAYDTRR
HAMSQKGRMD SVYKPSPPQL LYLDDDAWVK ATEGRRVLRF APIQQATGPG VIDAGGRIGR
NFSPERQQES ISLFAALATH VKDRMKAGPV VIASYSEGAR ERLSGLIEDE ALPRRSTCAT
GTAWASAGCH LAVWPLEHGF QTETVTVISE QDVLGERLIR RARKKRRADN FLTETQSLSA
GDLVVHVDHG VGRYHGLEVV TALGAAHECL LIEYAENAKL YLPVENIELL SRFGHEEGML
DKLGGGAWQA KKSKLKERIR QAAERLIRIA AERALRNAPK LIPHDSIWEQ FLARFPYDET
EDQLQAIEDV LNDMESGHPM DRLICGDVGF GKTEVAIRAA FVAAMSGVQV AVIAPTTLLA
RQHYKNFAER FRGFPINVRP LSRFVSAKEA AETRAGITSG QVDIVVGTHA LLAKSVKFRD
LGLLVIDEEQ HFGVNHKERL KALRTDVHVL TLTATPIPRT LQMSLTGVRD LSIIGTPPVD
RLAIRTYVSE FDAVTVREAL LRERFRGGQS FFVVPRVNDL PEIEAFLRDE VPEVSYVVAH
GQMAAGELDE RMNAFYDGRY DVLLATTIVE SGLDIPTANT MIVHRADMFG LAQLYQIRGR
VGRSKTRAYA YLTTRPRAPL TPGAEKRLRV LGSIDSLGAG FTLASQDLDI RGAGNLIGEE
QSGQMREVGF ELYQQMLEEQ ITKIRSGEAE GLSESEGQWA PQINLGVPVL IPEAYVPDLD
VRLGLYRRLS DLTTKVELEG FAAELIDRFG PLPREVNTLM LVVRIKAMCL RAGISQLDAG
PKGATIRFHN DKFVSPEGLV EFIQDQKGQA KVKDNKIVVM RDWSKEADKI RGAFGLAREL
AEKVKAAKKA KAET
//
