ID U3C6H9_9VIBR Unreviewed; 879 AA.
AC U3C6H9;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN ECO:0000313|EMBL:GAD77004.1};
GN ORFNames=VAZ01S_057_00390 {ECO:0000313|EMBL:GAD77004.1};
OS Vibrio azureus NBRC 104587.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1219077 {ECO:0000313|EMBL:GAD77004.1, ECO:0000313|Proteomes:UP000016567};
RN [1] {ECO:0000313|EMBL:GAD77004.1, ECO:0000313|Proteomes:UP000016567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 104587 {ECO:0000313|EMBL:GAD77004.1,
RC ECO:0000313|Proteomes:UP000016567};
RA Isaki S., Hosoyama A., Numata M., Hashimoto M., Hosoyama Y., Tsuchikane K.,
RA Noguchi M., Hirakata S., Ichikawa N., Ohji S., Yamazoe A., Fujita N.;
RT "Whole genome shotgun sequence of Vibrio azureus NBRC 104587.";
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAD77004.1}.
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DR EMBL; BATL01000057; GAD77004.1; -; Genomic_DNA.
DR AlphaFoldDB; U3C6H9; -.
DR STRING; 1219077.VAZ01S_057_00390; -.
DR eggNOG; COG2352; Bacteria.
DR Proteomes; UP000016567; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:GAD77004.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000016567}.
FT ACT_SITE 140
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 546
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 879 AA; 99609 MW; 3E73AD949EB209D5 CRC64;
MTMNEKYAAL KSNVRMLGHL LGNTIRDAHG EEIFEKVETI RTLSKSAQAG NQADRESLIE
EIKSLPDEQL TPVTRAFNQF LNLTNIAEQY HTISRHCEEH VCEPDAMNTL FSKLVQNKVS
KLDTAQAVKD LNIELVLTAH PTEITRRTMI NKLVKINECL SKLELSDLSF KERKKTERRL
EQLIAESWHS DVIRQQRPTP LDEAKWGFAV VENSLWEAVP EFLREMNDRL KPYLGEGLPI
DARPVHFSSW MGGDRDGNPF VTHSITREVL LLSRWKAADL YLNDINELIS ELSMTVCNEN
VRQLAGEDAH EPYRAILKQL RSLLIESKDI LDAKIHGQQL AVKAPLRNVQ QLWEPLYACY
KSLTECGMTV IANGSLLDTL RRVKAFGVHL VRLDIRQEST RHADALSELT RYLGIGDYEQ
WSEQDKVAFL TNELASKRPL LPRDWEPSEP VKEVLDTCKI IAAQPREAFG AYVISMARTA
SDVLAVHLLL QEAGCPYRMD VCPLFETLDD LNNAESVIQQ LMGIDLYRGF IQNHQMVMIG
YSDSAKDAGV MSAGWAQYHA MESLVKVAEN EGIELTLFHG RGGTIGRGGA PAHAALLSQP
PKSLKGGLRV TEQGEMIRFK LGLPDVAVNS FNLYASAILE ANLLPPPEPK QEWRELMEVL
SQVSCEAYRR VVRDEPDFVP YFRQATPELE LGKLPLGSRP AKRNPNGGVE SLRAIPWIFS
WSQNRLVLPA WLGAGEAIQY SINQGHQALL EEMCREWPFF STRLGMLEMV YSKCNIDISR
YYDQRLADES LLPLGERLRE QLQQDIKAVL NVENNENLMQ RNPWGLESIR LRNIYVEPLN
MLQAELLYRT RQTEQPSTHL EEALMVTIAG IAAGMRNTG
//
