ID U3CWH5_CALJA Unreviewed; 379 AA.
AC U3CWH5; A0A2R8M7P3;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=tRNA N(3)-methylcytidine methyltransferase {ECO:0000256|PIRNR:PIRNR037755};
DE EC=2.1.1.- {ECO:0000256|PIRNR:PIRNR037755};
GN Name=METTL2B {ECO:0000313|EMBL:JAB02365.1};
OS Callithrix jacchus (White-tufted-ear marmoset).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Platyrrhini; Cebidae;
OC Callitrichinae; Callithrix; Callithrix.
OX NCBI_TaxID=9483 {ECO:0000313|EMBL:JAB02365.1};
RN [1] {ECO:0000313|EMBL:JAB02365.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Bladder {ECO:0000313|EMBL:JAB02365.1}, Cerebellum
RC {ECO:0000313|EMBL:JAB45921.1}, Cerebral cortex
RC {ECO:0000313|EMBL:JAB24881.1}, Hippocampus
RC {ECO:0000313|EMBL:JAB23127.1}, and Skeletal muscle
RC {ECO:0000313|EMBL:JAB35031.1};
RX PubMed=25243066; DOI=10.1186/2047-217X-3-14;
RA Maudhoo M.D., Ren D., Gradnigo J.S., Gibbs R.M., Lubker A.C.,
RA Moriyama E.N., French J.A., Norgren R.B.Jr.;
RT "De novo assembly of the common marmoset transcriptome from NextGen mRNA
RT sequences.";
RL Gigascience 3:14-14(2014).
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase.
CC {ECO:0000256|PIRNR:PIRNR037755}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cytidine(32) in tRNA(Arg)(CCU) + S-adenosyl-L-methionine =
CC H(+) + N(3)-methylcytidine(32) in tRNA(Arg)(CCU) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:60912, Rhea:RHEA-COMP:15710, Rhea:RHEA-
CC COMP:15712, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74894, ChEBI:CHEBI:82748;
CC Evidence={ECO:0000256|ARBA:ARBA00036328};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60913;
CC Evidence={ECO:0000256|ARBA:ARBA00036328};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cytidine(32) in tRNA(Thr) + S-adenosyl-L-methionine = H(+) +
CC N(3)-methylcytidine(32) in tRNA(Thr) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:50960, Rhea:RHEA-COMP:12850, Rhea:RHEA-COMP:12852,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74894, ChEBI:CHEBI:82748;
CC Evidence={ECO:0000256|ARBA:ARBA00037010};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50961;
CC Evidence={ECO:0000256|ARBA:ARBA00037010};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. METL family.
CC {ECO:0000256|ARBA:ARBA00009725, ECO:0000256|PIRNR:PIRNR037755}.
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DR EMBL; GAMT01009497; JAB02364.1; -; mRNA.
DR EMBL; GAMT01009496; JAB02365.1; -; mRNA.
DR EMBL; GAMS01000009; JAB23127.1; -; mRNA.
DR EMBL; GAMR01009051; JAB24881.1; -; mRNA.
DR EMBL; GAMR01009050; JAB24882.1; -; mRNA.
DR EMBL; GAMQ01006820; JAB35031.1; -; mRNA.
DR EMBL; GAMQ01006819; JAB35032.1; -; mRNA.
DR EMBL; GAMP01006834; JAB45921.1; -; mRNA.
DR EMBL; GAMP01006833; JAB45922.1; -; mRNA.
DR EMBL; GAMP01006832; JAB45923.1; -; mRNA.
DR RefSeq; XP_002748147.3; XM_002748101.4.
DR AlphaFoldDB; U3CWH5; -.
DR STRING; 9483.ENSCJAP00000055096; -.
DR HOGENOM; CLU_029724_0_1_1; -.
DR InParanoid; U3CWH5; -.
DR OrthoDB; 5471626at2759; -.
DR Bgee; ENSCJAG00000033545; Expressed in heart and 6 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:UniProt.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:UniProt.
DR GO; GO:0001510; P:RNA methylation; IEA:UniProt.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR026113; METTL2/6/8-like.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22809; METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR22809:SF4; TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE METTL2A; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR PIRSF; PIRSF037755; Mettl2_prd; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Methyltransferase {ECO:0000256|PIRNR:PIRNR037755,
KW ECO:0000313|EMBL:JAB02365.1};
KW Transferase {ECO:0000256|PIRNR:PIRNR037755, ECO:0000313|EMBL:JAB02365.1};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 186..288
FT /note="Methyltransferase type 12"
FT /evidence="ECO:0000259|Pfam:PF08242"
SQ SEQUENCE 379 AA; 43726 MW; EA7B3D17A3DBE603 CRC64;
MASSYTEGVP AVLADKRQQF GSRLLSDPAR VFHHNAWDNV EWSEEQAAAA ERKVQENSTQ
RVCQEKQVDY EINAHKYWND FYKIHENGFF KDRHWLFTEF PELAPSQNQN HLKDWFLENK
RSEISECRNN EDGPVLIMEE QHKCSSKSLE HKTQVPPVEE NVTQKISDLE ICADEFPGSS
ATYRILEVGC GVGNTVFPIL QTNNDAGLFV YCCDFSSTAI ELVQTNSEYD PFRCFAFVHD
LCDEEKSYPV PEGSLDIIIL IFVLSAIVPD KMQKAINRLS KLLKPGGMML LRDYGRYDMA
QLRFKKGQCL SGNFYVRGDG TRVYFFTQEE LDVLFTTAGL EKVQNLVDRR LQVNRGKQLT
MYRVWIQCKY RKPLLSSTS
//
