ID U3JAJ7_DANRE Unreviewed; 2105 AA.
AC U3JAJ7; A0A8M1NAL3;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE SubName: Full=Si:dkey-61p9.11 {ECO:0000313|Ensembl:ENSDARP00000127265};
GN Name=fasn2 {ECO:0000313|ZFIN:ZDB-GENE-041210-242};
GN Synonyms=si:dkey-61p9.11 {ECO:0000313|RefSeq:NP_001041530.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000127265};
RN [1] {ECO:0000313|Ensembl:ENSDARP00000127265, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000127265};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000313|Ensembl:ENSDARP00000127265}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000127265};
RG Ensembl;
RL Submitted (SEP-2013) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:NP_001041530.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001041530.1};
RX PubMed=30974150;
RA Thiessen K.D., Grzegorski S.J., Chin Y., Higuchi L.N., Wilkinson C.J.,
RA Shavit J.A., Kramer K.L.;
RT "Zebrafish otolith biomineralization requires polyketide synthase.";
RL Mech. Dev. 157:1-9(2019).
RN [4] {ECO:0000313|RefSeq:NP_001041530.1}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001041530.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP];
CC Xref=Rhea:RHEA:41792, Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9685,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:64479,
CC ChEBI:CHEBI:78449; EC=2.3.1.39;
CC Evidence={ECO:0000256|ARBA:ARBA00023404};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41793;
CC Evidence={ECO:0000256|ARBA:ARBA00023404};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194}.
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DR EMBL; AL935186; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001041530.1; NM_001048065.1.
DR STRING; 7955.ENSDARP00000127265; -.
DR PaxDb; 7955-ENSDARP00000127265; -.
DR Ensembl; ENSDART00000152922; ENSDARP00000127265; ENSDARG00000096762.
DR Ensembl; ENSDART00000152922.2; ENSDARP00000127265.1; ENSDARG00000096762.2.
DR GeneID; 100000781; -.
DR KEGG; dre:100000781; -.
DR AGR; ZFIN:ZDB-GENE-041210-242; -.
DR ZFIN; ZDB-GENE-041210-242; fasn2.
DR eggNOG; KOG1202; Eukaryota.
DR HOGENOM; CLU_000022_35_2_1; -.
DR OMA; IINHCTG; -.
DR OrthoDB; 5396558at2759; -.
DR PhylomeDB; U3JAJ7; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000000437; Alternate scaffold 4.
DR Proteomes; UP000000437; Chromosome 4.
DR Bgee; ENSDARG00000096762; Expressed in intestine and 6 other cell types or tissues.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0004314; F:[acyl-carrier-protein] S-malonyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004312; F:fatty acid synthase activity; IBA:GO_Central.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IBA:GO_Central.
DR CDD; cd05274; KR_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR45681:SF6; CARRIER DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR45681; POLYKETIDE SYNTHASE 44-RELATED; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 1.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF47336; ACP-like; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..431
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2014..2089
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 2105 AA; 233600 MW; D8736E456B71C31D CRC64;
MDDDTEIAIV GVGCHFPGGE GLENFWRVLL EGKNCAVQIP NDRFNLSQWY DPEESKAGKT
YTAKAALIDG LNAFDHKFFG ITEAESLSMD PQHKLLLQCT YRALEDAGIP MEKASGTRTG
VYLGLMNRDF ELATVRNNPK HISHTTGTGV AMSIAANRIS YIFNFTGPSV AIDCACSSSL
VALHFACQAI KQGDCDMSVC GGVSCILEPA LFVALSKAKM ISPDGTSKPF SSKADGYGRG
EGCGVVLLKP LKRALEDHDH IWGVIGKTAV NQDGHSVSPI TKPSMVQQEE LLRKIYSTEA
DLCSVQYIEA HGTGTPVGDP VEAGSMSKVI AKERSQKSGP LIIGSVKSNI GHTESAAGVA
GLIKVLLMMQ HETIVPSLFY SKENSSIDLK SLNMNIPTRV QKWISNNRAG RIAGINNFGF
GGTNAHAIVK QYVQSKKPKS QPISKSCQYF VVSAASEKSM RNIIRDTADQ IGAGKISDLQ
SLLYTSACRR SHLKHKYRNA FQTSSLADLQ DKLTAAVDKR LVLSKTDSRV VFVFCGNGVT
YQGMCQQLLK HETVFREEIV KIETLLKIYR RQNLIEMLEG RSEMSKDLSD PQLVQPLLFA
IQVAVFKLLK HWGISPDAVL GHSIGEVAAA HCSGLLSLED AVKVIHYRSS LQSTVTGGKM
LVVSNMAVSE ILNMLPSHSG RVCLAAYNSP QSCTLSGDAA EIDSLHQKLS SSTNGKDLFL
RILDVPAAYH SHMMDPIVSR VKEDIGSLQT CDLEMELFST VTGVSSCSTD FITGEYWARN
IRQAVEFEQA VKSATKNKRN VIFVEIGPRR SLQRYITETL GKDIAVVPAV QPDKDHETML
AVVSKLFELG ANINWDLFYK GWEAEPIPFP RYQFDDVKRE VFASDLQLTG TTRNHPVVTQ
MSTDSSVFSC DLSSESVAFL QDHKHNGVAI IPGAFYTELG LATFMEYAKP KVPLSSLQLS
ITFQSPLTFT QNAPHIKVQL ENLADSMHNF KIHSTSAVYA FGSVEVQPGR RPEEQFISLD
SISRRCTLHM TSEEFYKQLS QTGFQYGSVF RNKADVYCGE EFREALSVVK VPKELLHQLH
DYHLHPVVLD YFMQLAPVTL GNDQSARPQF PAQIASLTVF EPLQEELVVY LRAVDVGDDG
FEICGCLANK QGRVLVEIKH VKIKMIGSRS EVVKEYFFHN SFDIISEDDM LDTQIKALVF
SDQLGIFKAL QQHLDSRSRC VSFSDSSTLL EGGVETLLSK LAISSVKKNF QEILFMWSDA
DLTTLESEKV LESMAGCCEM FRKTVRYLKA LQFPGVIRVI TYRSSESIVD SINPGYVLTG
MTRACAAELQ ELSFQLIDMG SGTSEDIKAL VQVLRSYPCH KYPELVVKEG NILQPEITHT
PLPSMASPLQ KVHMLDEELF ILQTSDPYAM RNLSATEMEN SIQPIQGKHV ELHLNKICVH
SSDYFPVSMS DLSYGHTLYW NKHTSQHHNL LALDYSGTVT AVGKDVSKFK VGDHVVSCYP
VSATSKVVIP AAVCCKAKRL SFLNDIPCVS YMVLAWEILH KALPKIKVQR KLGIFSTVPD
SALINVLLAI ANRSGWNVKV CTQTDQLSCD FSEVVGAVFL PPYNLVTAEV ASRIKGINDI
VFVCDNQTEV PLNSATFRST NEEVHFHFVF MSQVLQKWSL QTQMPKIYRW QKSMHLNRKS
MSLDTTAVQR ANSQHIDLLS VKESDSYFTC QILPIIILNS EDKNQLSEIP VIPKNNLFKK
KSVYIVTGGL TGLGFETVKF IAQKGGEHIV ILSRRNPNPE MQQEISQLSS RCGSVIKSLP
CDVSVSEQVD QTIAKVKKLF PSSPIKGVFH SAVVLHDGLI ERLDKSLYEK VMRPKVNGVI
NLHRATIQCN LDYFVCYSSI SAFMGNASQT NYAAANTFMD TFCQYRRNIG LAGQSINWGA
LNLGLLLDKV HFQRFLEAKG ILLLEVPEIH ESLEQCLLIN KPQQVVCRFS FKNSWNSVLS
QNKSLNARLF KIAKEAFIRA GVNPSRPEQP MTSSSPHDYL RFLISETAGV EKDELSDDVH
LFELGIDSML AMTLQNHIFS YRGVNIPLVT LLDPNSTLST ITKILEENGV DEYQSASYES
TSTYL
//
