ID U4KHG4_9VIBR Unreviewed; 397 AA.
AC U4KHG4;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE SubName: Full=Putative Astacin (Astacus proteinase, Crayfish small-molecule proteinase) {ECO:0000313|EMBL:CCO61229.1};
DE EC=3.4.24.21 {ECO:0000313|EMBL:CCO61229.1};
GN ORFNames=VIBNI_B1478 {ECO:0000313|EMBL:CCO61229.1};
OS Vibrio nigripulchritudo.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=28173 {ECO:0000313|EMBL:CCO61229.1, ECO:0000313|Proteomes:UP000016895};
RN [1] {ECO:0000313|EMBL:CCO61229.1, ECO:0000313|Proteomes:UP000016895}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SnF1 {ECO:0000313|Proteomes:UP000016895};
RX PubMed=23739050; DOI=10.1038/ismej.2013.90;
RA Goudenege D., Labreuche Y., Krin E., Ansquer D., Mangenot S., Calteau A.,
RA Medigue C., Mazel D., Polz M.F., Le Roux F.;
RT "Comparative genomics of pathogenic lineages of Vibrio nigripulchritudo
RT identifies virulence-associated traits.";
RL ISME J. 7:1985-1996(2013).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU01211};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PROSITE-
CC ProRule:PRU01211};
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01211}.
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DR EMBL; FO203527; CCO61229.1; -; Genomic_DNA.
DR RefSeq; WP_004397990.1; NC_022543.1.
DR AlphaFoldDB; U4KHG4; -.
DR STRING; 28173.VIBNI_B1478; -.
DR KEGG; vni:VIBNI_B1478; -.
DR PATRIC; fig|1260221.3.peg.5078; -.
DR eggNOG; COG3170; Bacteria.
DR eggNOG; COG3227; Bacteria.
DR OrthoDB; 5903218at2; -.
DR Proteomes; UP000016895; Chromosome 2.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04280; ZnMc_astacin_like; 1.
DR Gene3D; 2.60.120.380; -; 1.
DR Gene3D; 3.40.390.10; Collagenase (Catalytic Domain); 1.
DR InterPro; IPR034035; Astacin-like_dom.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR007280; Peptidase_C_arc/bac.
DR InterPro; IPR001506; Peptidase_M12A.
DR InterPro; IPR006026; Peptidase_Metallo.
DR PANTHER; PTHR10127:SF887; ASTACIN-LIKE METALLOENDOPEPTIDASE; 1.
DR PANTHER; PTHR10127; DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING; 1.
DR Pfam; PF01400; Astacin; 1.
DR Pfam; PF04151; PPC; 1.
DR PRINTS; PR00480; ASTACIN.
DR SMART; SM00235; ZnMc; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
DR PROSITE; PS51864; ASTACIN; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU01211,
KW ECO:0000313|EMBL:CCO61229.1};
KW Metal-binding {ECO:0000256|PROSITE-ProRule:PRU01211};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049, ECO:0000256|PROSITE-
KW ProRule:PRU01211}; Protease {ECO:0000256|PROSITE-ProRule:PRU01211};
KW Reference proteome {ECO:0000313|Proteomes:UP000016895};
KW Zinc {ECO:0000256|PROSITE-ProRule:PRU01211}.
FT DOMAIN 90..287
FT /note="Peptidase M12A"
FT /evidence="ECO:0000259|PROSITE:PS51864"
FT ACT_SITE 185
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
FT BINDING 184
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
FT BINDING 188
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
FT BINDING 194
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01211"
SQ SEQUENCE 397 AA; 43068 MW; CB6E52D2016EABB4 CRC64;
MKLNVVIPVS VLTLTPIIAV GASESDVNAT VPILDTQQTH IIGTNDVVSY DVKSLEGGEA
AVFQGDIVLG NAKDVQNLGV QPMRFANEGE VTGYASWSGH AKWPNGVVPY TIASNIPAHD
VASIKEGMRW IENVSNVRFV ERSNQTGYVR IIRDRGCYSS VGYTGRAQNL SIGQGCGTPG
IVAHEFLHAL GFWHEQSRAD RDQYVNIHWN NIEQGKEHNF RKKGAVTTSI GPYDVRSIMH
YSYRSFSING QPTITSKDTS VPNNQLGQRQ RLTEHDIAAL QETYGGGGTT PPNPGGPEPI
NETLTNVGVE RGAWHHHVQE IGSGYKKLTI TTSGGSGDVD LYVRKGSQPT LSQHDCRPWR
NGNAESCVID NPGAGKWHIG INGYRASSGV TVTIKAE
//
