ID U4KUM3_PYROM Unreviewed; 491 AA.
AC U4KUM3;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|ARBA:ARBA00017788, ECO:0000256|RuleBase:RU368004};
DE EC=2.1.1.211 {ECO:0000256|ARBA:ARBA00012795, ECO:0000256|RuleBase:RU368004};
GN ORFNames=PCON_03668 {ECO:0000313|EMBL:CCX04777.1};
OS Pyronema omphalodes (strain CBS 100304) (Pyronema confluens).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Pyronemataceae; Pyronema.
OX NCBI_TaxID=1076935 {ECO:0000313|EMBL:CCX04777.1};
RN [1] {ECO:0000313|EMBL:CCX04777.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS100304 {ECO:0000313|EMBL:CCX04777.1};
RC TISSUE=Vegetative mycelium {ECO:0000313|EMBL:CCX04777.1};
RX PubMed=24068976; DOI=10.1371/journal.pgen.1003820;
RA Traeger S., Altegoer F., Freitag M., Gabaldon T., Kempken F., Kumar A.,
RA Marcet-Houben M., Poggeler S., Stajich J.E., Nowrousian M.;
RT "The genome and development-dependent transcriptomes of Pyronema confluens:
RT a window into fungal evolution.";
RL PLoS Genet. 9:E1003820-E1003820(2013).
CC -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00000660,
CC ECO:0000256|RuleBase:RU368004};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368004}.
CC -!- SIMILARITY: Belongs to the TRM44 family.
CC {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
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DR EMBL; HF935217; CCX04777.1; -; Genomic_DNA.
DR AlphaFoldDB; U4KUM3; -.
DR STRING; 1076935.U4KUM3; -.
DR eggNOG; KOG3790; Eukaryota.
DR OMA; IREPNIN; -.
DR OrthoDB; 1353816at2759; -.
DR Proteomes; UP000018144; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW Methyltransferase {ECO:0000256|RuleBase:RU368004,
KW ECO:0000313|EMBL:CCX04777.1};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW tRNA processing {ECO:0000256|RuleBase:RU368004}.
SQ SEQUENCE 491 AA; 54952 MW; D56E1D048F63A212 CRC64;
MSEFPAHPPS LHALDHSNNQ QTWQPILTEP FPFAPSSFLA ALEQMSQHPE HNTSWILRSD
TLYDSTDPSS VPGDSDIRKI QTPGYAITRT LTRRFIPRNP QRDAYAEQTI HFLTGTDLSG
EIRELIVYLN HCDTAEGTPY YLPKVRGVAW LLTPLEAGEE EGEGEERRKY KITLLYSLFT
SPPDERLKRT ALHLLARATK LAAGIESGYI KRVHHDLVVK REGFQDRYLA LKAKHAARLV
EGWRERTDPK KHVFEDILIA TFLVCLWEGM YPDKKGWVGF ADIGCGNGVL VDILIREGWS
GCGFDLRARK SWGGFCEETQ AVLKERILVP FLLGDEAEEG DHDGRFQEGT FIISNHADEL
TPWTPLLAAA NRCPFLAIPC CSHDLSGAKK RFPPPKELEG TEAKGDLRLN MNLSKSTYAS
LCAYVEGIAK KCGWAVEREI LRIPSTRNLG IIGRKLVDEE VDIAEVLQSE GGGDGWRIAV
AALKKKEAAG H
//
