ID   U4LH50_PYROM            Unreviewed;       672 AA.
AC   U4LH50;
DT   11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT   11-DEC-2013, sequence version 1.
DT   27-MAR-2024, entry version 39.
DE   RecName: Full=tyrosinase {ECO:0000256|ARBA:ARBA00011906};
DE            EC=1.14.18.1 {ECO:0000256|ARBA:ARBA00011906};
GN   ORFNames=PCON_09433 {ECO:0000313|EMBL:CCX30832.1};
OS   Pyronema omphalodes (strain CBS 100304) (Pyronema confluens).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC   Pezizales; Pyronemataceae; Pyronema.
OX   NCBI_TaxID=1076935 {ECO:0000313|EMBL:CCX30832.1};
RN   [1] {ECO:0000313|EMBL:CCX30832.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS100304 {ECO:0000313|EMBL:CCX30832.1};
RC   TISSUE=Vegetative mycelium {ECO:0000313|EMBL:CCX30832.1};
RX   PubMed=24068976; DOI=10.1371/journal.pgen.1003820;
RA   Traeger S., Altegoer F., Freitag M., Gabaldon T., Kempken F., Kumar A.,
RA   Marcet-Houben M., Poggeler S., Stajich J.E., Nowrousian M.;
RT   "The genome and development-dependent transcriptomes of Pyronema confluens:
RT   a window into fungal evolution.";
RL   PLoS Genet. 9:E1003820-E1003820(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 L-dopa + O2 = 2 H2O + 2 L-dopaquinone; Xref=Rhea:RHEA:34287,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57504,
CC         ChEBI:CHEBI:57924; EC=1.14.18.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000426};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tyrosine + O2 = H2O + L-dopaquinone; Xref=Rhea:RHEA:18117,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57924,
CC         ChEBI:CHEBI:58315; EC=1.14.18.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001038};
CC   -!- COFACTOR:
CC       Name=Cu(2+); Xref=ChEBI:CHEBI:29036;
CC         Evidence={ECO:0000256|ARBA:ARBA00001973};
CC   -!- SIMILARITY: Belongs to the tyrosinase family.
CC       {ECO:0000256|ARBA:ARBA00009928}.
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DR   EMBL; HF935497; CCX30832.1; -; Genomic_DNA.
DR   AlphaFoldDB; U4LH50; -.
DR   STRING; 1076935.U4LH50; -.
DR   eggNOG; ENOG502RF2C; Eukaryota.
DR   OrthoDB; 1908494at2759; -.
DR   Proteomes; UP000018144; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004503; F:tyrosinase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1280.10; Di-copper center containing domain from catechol oxidase; 1.
DR   InterPro; IPR008922; Di-copper_centre_dom_sf.
DR   InterPro; IPR041640; Tyrosinase_C.
DR   InterPro; IPR002227; Tyrosinase_Cu-bd.
DR   PANTHER; PTHR11474:SF76; TYROSINASE; 1.
DR   PANTHER; PTHR11474; TYROSINASE FAMILY MEMBER; 1.
DR   Pfam; PF18132; Tyosinase_C; 1.
DR   Pfam; PF00264; Tyrosinase; 1.
DR   PRINTS; PR00092; TYROSINASE.
DR   SUPFAM; SSF48056; Di-copper centre-containing domain; 1.
DR   PROSITE; PS00498; TYROSINASE_2; 1.
PE   3: Inferred from homology;
KW   Melanin biosynthesis {ECO:0000256|ARBA:ARBA00023101};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           20..672
FT                   /note="tyrosinase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004652006"
FT   DOMAIN          319..330
FT                   /note="Tyrosinase copper-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS00498"
SQ   SEQUENCE   672 AA;  73227 MW;  62D0C0837005BB54 CRC64;
     MKTWTITAVA ISLVSLVSAT PLVEKRQAQT STPVTGASTG GVQQRLSIQN LQANQDVFNM
     YILGLDAMQR MPETNSLSYY QIAGIHGRPY IPWQSAVTAQ QDNTVGYCTH SSALFVTWHR
     PYIALIEQRV VAMAVEEAAK FSGADRKRYA AAAQKVRLPY WDWAAEDTRS AIPAVMMQPN
     IQVSRPNPRT GVAQRVTIRN PLFAYNFSNQ QLQRQYFQGF FQTTPRTLRS PISPRADNNA
     ASDATMRIQY PARRQNTYNL FSIPTFREFS STAFSPGGQP NAWTSVESIH NQIHAALGGR
     GVTPGSAGHM STVDYSAFDP IFWLHHVQVD RLSAMYQATH PGQVVTPQNA TPVFARRVNA
     QTMDTIDTPL WPFRKANRQY YTSRDVSTAG SIWGLGYAYP EVPVSYQNSP QEDLASFVTS
     RINALYSPAS SPAAPGARAL VPGVNAVERQ EYSVQYQTQA AKKPDNAFSA IAAPGNGKTS
     REWLCHFVFT PAEIGAGASP QLDVYFANAS NATTRATDAV ASLIENPAFN RTSGDYYVGS
     GAGIGKLEYT EADRNMKITA TVPLNQALTD MNIDPNNVAA CVKYLADNLV WRIMSDGGGV
     VDLAKVPSLK VGVSSAEVTY GAAHELPKWG KWETYYEVTE KKSCGLMTGD SALLGSASAP
     RYLGRNGGGG GN
//