ID   U4LIR9_PYROM            Unreviewed;       944 AA.
AC   U4LIR9;
DT   11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT   11-DEC-2013, sequence version 1.
DT   27-MAR-2024, entry version 46.
DE   RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE            EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE   AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN   ORFNames=PCON_10779 {ECO:0000313|EMBL:CCX31432.1};
OS   Pyronema omphalodes (strain CBS 100304) (Pyronema confluens).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC   Pezizales; Pyronemataceae; Pyronema.
OX   NCBI_TaxID=1076935 {ECO:0000313|EMBL:CCX31432.1};
RN   [1] {ECO:0000313|EMBL:CCX31432.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS100304 {ECO:0000313|EMBL:CCX31432.1};
RC   TISSUE=Vegetative mycelium {ECO:0000313|EMBL:CCX31432.1};
RX   PubMed=24068976; DOI=10.1371/journal.pgen.1003820;
RA   Traeger S., Altegoer F., Freitag M., Gabaldon T., Kempken F., Kumar A.,
RA   Marcet-Houben M., Poggeler S., Stajich J.E., Nowrousian M.;
RT   "The genome and development-dependent transcriptomes of Pyronema confluens:
RT   a window into fungal evolution.";
RL   PLoS Genet. 9:E1003820-E1003820(2013).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR   EMBL; HF935596; CCX31432.1; -; Genomic_DNA.
DR   AlphaFoldDB; U4LIR9; -.
DR   STRING; 1076935.U4LIR9; -.
DR   eggNOG; KOG0433; Eukaryota.
DR   OMA; PVYWGTE; -.
DR   OrthoDB; 656at2759; -.
DR   Proteomes; UP000018144; Unassembled WGS sequence.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR   CDD; cd00818; IleRS_core; 1.
DR   Gene3D; 1.10.730.20; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR   Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR033708; Anticodon_Ile_BEm.
DR   InterPro; IPR002301; Ile-tRNA-ligase.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   NCBIfam; TIGR00392; ileS; 1.
DR   PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   PRINTS; PR00984; TRNASYNTHILE.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR   SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW   ECO:0000256|RuleBase:RU363035};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU363035};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW   ECO:0000256|RuleBase:RU363035}.
FT   DOMAIN          51..688
FT                   /note="Aminoacyl-tRNA synthetase class Ia"
FT                   /evidence="ECO:0000259|Pfam:PF00133"
FT   DOMAIN          731..894
FT                   /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT                   anticodon-binding"
FT                   /evidence="ECO:0000259|Pfam:PF08264"
SQ   SEQUENCE   944 AA;  105266 MW;  920D4A16495AA030 CRC64;
     MQRCFRRFHG TLPSAGVSLK DTLRLPTTTF PIVPSDSKTE RASLLKKITS DLYIQQSQRD
     DAPTFILHDG PPYANGALHI GHALNKILKD VINRVQVLEG KKVVFQPGWD CHGLPIELKA
     LQAKQEKAGT KLDKSAAKAA KGAVEMGKSM SALEIRGIAR ELASGTIEQQ MSNFKDWGIM
     ADWEGKYVTM DKDYEMRQLE VFRDMLKKEL IYRKNRPVYW SPSSGSALAE AELEYDPAYK
     SQSAFVKFPL QQEIGGQKDV SAVIWTTTPW TIPANKAIAV GEKIEYCIVQ SAKHGKLLIA
     SEAIADLQKK LEEEFTIIAG PMPGTELVGS NYTHPLLSEA APQPILSADF VTAESGTGLV
     HLAPGHGMDD YLVCLKHEIP PFSPVDNNGC YTADTLPSLV GKEVLYGGQK AVIALMEESN
     CLIFQHKFEH KYPLDWRTKS PVIVRATAQW FANAGGIKEA AHTALENVTF LPKTGATRLN
     SFVQNRSEWC ISRQRAWGVP IPALYDADSG EPLLTEESVN HIITVLNEKG TDSWFDPSVP
     NEVFVSPEFA NDGKQYIRGK ETMDVWFDSG TSWATLASRL GQPDVTADIY LEGSDQHRGW
     FQSSLITSLA TREQAPFKQV ITHGFILDGK GKKMSKSLGN VVSPADLTLK APTDINLPKP
     QKPLAKMQRI DILRLWVAFC DFTRDISVGP KVVSQVHEIL RKARVTTRFC LGVLEDWDGI
     YTPYSELGKL DKIALAHLAQ VNNKVRESYA QYNFNKAVSA LAQYTTNDLS AFYLDVIKDR
     LYTLSPEDLS RRSAQTTIAY VLRNYLSLLS PLIPLLVQEV WSHAPKSLLR STPQEERQML
     EYFSAAEGWE MNWADGKLIT DFENLKTLHD AVKQGIERAK TAGNAKVNLE AGVEIVVPKG
     GYAEKFVKSY LEELEQWLLF LMWRWWWRGG GRGGPRGGGL VREG
//