ID U4LK46_PYROM Unreviewed; 1065 AA.
AC U4LK46;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=tRNA wybutosine-synthesizing protein 4 {ECO:0000256|ARBA:ARBA00018045};
DE EC=2.1.1.290 {ECO:0000256|ARBA:ARBA00012779};
DE EC=2.3.1.231 {ECO:0000256|ARBA:ARBA00012155};
DE AltName: Full=Leucine carboxyl methyltransferase 2 {ECO:0000256|ARBA:ARBA00030231};
DE AltName: Full=tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase {ECO:0000256|ARBA:ARBA00030847};
DE AltName: Full=tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase {ECO:0000256|ARBA:ARBA00029750};
GN ORFNames=PCON_13237 {ECO:0000313|EMBL:CCX32474.1};
OS Pyronema omphalodes (strain CBS 100304) (Pyronema confluens).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Pyronemataceae; Pyronema.
OX NCBI_TaxID=1076935 {ECO:0000313|EMBL:CCX32474.1};
RN [1] {ECO:0000313|EMBL:CCX32474.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS100304 {ECO:0000313|EMBL:CCX32474.1};
RC TISSUE=Vegetative mycelium {ECO:0000313|EMBL:CCX32474.1};
RX PubMed=24068976; DOI=10.1371/journal.pgen.1003820;
RA Traeger S., Altegoer F., Freitag M., Gabaldon T., Kempken F., Kumar A.,
RA Marcet-Houben M., Poggeler S., Stajich J.E., Nowrousian M.;
RT "The genome and development-dependent transcriptomes of Pyronema confluens:
RT a window into fungal evolution.";
RL PLoS Genet. 9:E1003820-E1003820(2013).
CC -!- FUNCTION: Probable S-adenosyl-L-methionine-dependent methyltransferase
CC that acts as a component of the wybutosine biosynthesis pathway.
CC Wybutosine is a hyper modified guanosine with a tricyclic base found at
CC the 3'-position adjacent to the anticodon of eukaryotic phenylalanine
CC tRNA. May methylate the carboxyl group of leucine residues to form
CC alpha-leucine ester residues. {ECO:0000256|ARBA:ARBA00025588}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in
CC tRNA(Phe) + CO2 + S-adenosyl-L-methionine = 2 H(+) + S-adenosyl-L-
CC homocysteine + wybutosine(37) in tRNA(Phe); Xref=Rhea:RHEA:37119,
CC Rhea:RHEA-COMP:11844, Rhea:RHEA-COMP:11847, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73544, ChEBI:CHEBI:74275; EC=2.3.1.231;
CC Evidence={ECO:0000256|ARBA:ARBA00000401};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-
CC adenosyl-L-methionine = 7-[(3S)-(3-amino-3-
CC methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36903, Rhea:RHEA-COMP:10379, Rhea:RHEA-
CC COMP:11844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73543,
CC ChEBI:CHEBI:74275; EC=2.1.1.290;
CC Evidence={ECO:0000256|ARBA:ARBA00001806};
CC -!- PATHWAY: tRNA modification; wybutosine-tRNA(Phe) biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004797}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LCMT family.
CC {ECO:0000256|ARBA:ARBA00010703}.
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DR EMBL; HF935878; CCX32474.1; -; Genomic_DNA.
DR AlphaFoldDB; U4LK46; -.
DR STRING; 1076935.U4LK46; -.
DR eggNOG; KOG2508; Eukaryota.
DR eggNOG; KOG2918; Eukaryota.
DR OMA; WEVDFPD; -.
DR OrthoDB; 9938at2759; -.
DR UniPathway; UPA00375; -.
DR Proteomes; UP000018144; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.650; Cupin; 1.
DR Gene3D; 2.120.10.80; Kelch-type beta propeller; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR041667; Cupin_8.
DR InterPro; IPR003347; JmjC_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR007213; Ppm1/Ppm2/Tcmp.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR46529; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4; 1.
DR PANTHER; PTHR46529:SF1; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4; 1.
DR Pfam; PF13621; Cupin_8; 1.
DR Pfam; PF13418; Kelch_4; 1.
DR Pfam; PF04072; LCM; 1.
DR SMART; SM00558; JmjC; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF117281; Kelch motif; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51184; JMJC; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:CCX32474.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:CCX32474.1};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 899..1050
FT /note="JmjC"
FT /evidence="ECO:0000259|PROSITE:PS51184"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1065 AA; 118496 MW; 97B5CB0F58C403B6 CRC64;
MSSPDVDTEP KSDTQHQQTA PTQQNQQTSP PPPPPTTNGT LQDAAATPAA TPATATNPNP
NAKGKPKISE KAKLDAAVIG TNNSSITSKR SVERLYSHED EQQFLRYFVK KPQRRSPILM
QAVEYVVHSF LQEELPHLSM RQKKVVVNLG CGYDPLPFQC LAKEKPNAEV LYVDIDYPDL
ITIKAGIIAQ TPGLNGLLTD KQHHETPVDH VHYQSNEYIA LGCDLRDLDT LQRLFEKHGL
VDHAVFFTAE VSLTYMAKDS VDALIAWAAS LPDARFSILE QIMPDGPHHP FARTMLKHFN
ALNTPLKSIS AYPRLEDQVK RYSSRGWKSV DACDLLAFWS NRIPQSEKER IDAIPGEPFD
EFEEFFIFCQ HYFILCAHNI SNPHTPFNGP GLSWNAGGAY HRYPSSSNHG PYPGINMTGN
KEKTLVAEYT PSPHLKRRFG AAAATGKDSF IYHGGQGQTT RLGSALCITH SAEGIQLDKQ
TSPSARVCHT MTTMPNGKIL LVGGRTSPDK PVADTWLLEG GVWRRVQDLP VARYRHTAAV
PGYQNTGGNQ VLIYGGRGGN RSVLGDFWLW CEEKGWREVM IQPIGSGSQR PPPRFATSMC
WTDRKYGAMG GGLDAKGNVL DDFWRLELHQ DPETGSETIK ALWSMVNMIA SKNLWKRFGG
QMVYAGNGSI LYVGGVSGRG LMRREDEILQ VCCNKVVVKG VRAAFPQENA PFLVGHSVVV
LQDRVIISGG GGVCFSMGSC TNDGIWSITN EEMCDSWRFI EEGYAPDDVD VPMPDSPAPY
TPDQDNATNE TIECKPLPRV TISTPEEFSR IVSEGLPVVL ENLTLGPCLN KWIPSYLSTM
IGDEKIVVHM SKSSAMSFQQ KNFKYIPMAF NDFITSAFPL TESPNNARIY FRSLSQSKPR
DKPTRLSDDF PQIAADFILP ATLKMVSENI HSSPLRISSA DVGMWLHYDG MANVLCHISG
RKLFRLYPPR DVSRLSFPHG ETTSTIPDIF AKYIPGTTPY EVELGKGDVV YIPPLWLHAA
KPLTPCVAVN VFLGIWDRML MLLGRMFMRQ EIFKRMKRGG RCWRG
//
