ID U5C257_9BACT Unreviewed; 796 AA.
AC U5C257;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Penicillin amidase {ECO:0008006|Google:ProtNLM};
GN ORFNames=P872_05170 {ECO:0000313|EMBL:ERM83006.1};
OS Rhodonellum psychrophilum GCM71 = DSM 17998.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Cytophagaceae;
OC Rhodonellum.
OX NCBI_TaxID=1123057 {ECO:0000313|EMBL:ERM83006.1, ECO:0000313|Proteomes:UP000016843};
RN [1] {ECO:0000313|EMBL:ERM83006.1, ECO:0000313|Proteomes:UP000016843}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GCM71 {ECO:0000313|EMBL:ERM83006.1,
RC ECO:0000313|Proteomes:UP000016843};
RX PubMed=24309741;
RA Hauptmann A.L., Glaring M.A., Hallin P.F., Prieme A., Stougaard P.;
RT "Draft Genome Sequence of the Psychrophilic and Alkaliphilic Rhodonellum
RT psychrophilum Strain GCM71T.";
RL Genome Announc. 1:e01014-13(2013).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERM83006.1}.
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DR EMBL; AWXR01000019; ERM83006.1; -; Genomic_DNA.
DR RefSeq; WP_019598779.1; NZ_KB906682.1.
DR AlphaFoldDB; U5C257; -.
DR MEROPS; S45.003; -.
DR PATRIC; fig|1123057.7.peg.2140; -.
DR eggNOG; COG2366; Bacteria.
DR OrthoDB; 9759796at2; -.
DR Proteomes; UP000016843; Unassembled WGS sequence.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF5; PENICILLIN G ACYLASE; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000016843};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT ACT_SITE 250
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 322
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 325
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 796 AA; 89923 MW; 350DC58E1136D2C0 CRC64;
MKKLVLVLLF MLILGTVGYQ IAKFNFGPKY NGEITLEGLN AETEVFFDDF GVPHIYASSA
EDAYQTLGYV HAQDRLFQME MMRRVGTGTL AELLGEDLLD VDKFFRTLGI PQHAQMSTAA
WHSEGDSEWK SATEAYIKGV NQFILEGKIP IEYTLLGSKP RDFTINDVHA VVGYMSFTFA
MAVKTDPLIT KMSRQLGPDY MKALSVHTLP EHHIIPNNYP KRDSVSLQIK TNTSLACLLE
KLPVPLFMGS NAWVIAPSRT ASGEVIFCND THIGFSQPSV WYEAHLEYPG FSYYGNHLAG
VPFGLVGHTR THSIGLTMFE NDDQDFFEEK LNPNNPNETI YGTGFSPIKS RMDTIKIKDK
DHVLFEIRET NHGPILNAVV PEIAGMTDNP VASWWVYVLE PTRAIEATWK MGHAKNIQEV
EDGARLIHAP GLNIMYGDKT GNIAWWASAK LPIRPAHVNS KIFLDGTNPL DEHTGWIPFE
ENPMSINPES GFVATSNNQP DTLSNGMYYP GYYYPGDRWD RISKTIMSRN DWTQESIKTL
QLESINETQP KNAKLLLSEI SKTDFPEDEA ILNALGDWSG EHGLEEIAPT LYYKWIYHIL
SEMMVDELGE EDFQNYLGTF MAVRSTPLLL QSENSPWWDN KNTPKIENRK DIINKALSKS
LTELHAQHGS NSRKWQWRKS ILSEHPHPLG AKKPLDRIFN VSTNPMAANE ESVNKLAFTL
NGEGIYKVNS GPAMRIILDF ANVDASESIL PTGQSGNLFS PWYKDQAAMF VEGHYRPQLM
KEEVIKGTYR KKLVFK
//