ID U5DDA8_AMBTC Unreviewed; 619 AA.
AC U5DDA8;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=AMTR_s00066p00128970 {ECO:0000313|EMBL:ERN20205.1};
OS Amborella trichopoda.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Amborellales; Amborellaceae; Amborella.
OX NCBI_TaxID=13333 {ECO:0000313|EMBL:ERN20205.1, ECO:0000313|Proteomes:UP000017836};
RN [1] {ECO:0000313|Proteomes:UP000017836}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24357323;
RG Amborella Genome Project;
RT "The Amborella genome and the evolution of flowering plants.";
RL Science 342:1241089-1241089(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; KI392060; ERN20205.1; -; Genomic_DNA.
DR RefSeq; XP_006858738.1; XM_006858676.2.
DR AlphaFoldDB; U5DDA8; -.
DR STRING; 13333.U5DDA8; -.
DR EnsemblPlants; ERN20205; ERN20205; AMTR_s00066p00128970.
DR GeneID; 18448615; -.
DR Gramene; ERN20205; ERN20205; AMTR_s00066p00128970.
DR KEGG; atr:18448615; -.
DR eggNOG; ENOG502QSIM; Eukaryota.
DR HOGENOM; CLU_008926_1_3_1; -.
DR OMA; SIPNDHK; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000017836; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF64; ENDOGLUCANASE 7; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059}; Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000017836};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 77..96
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 112..583
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 514
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 561
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 570
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 619 AA; 68771 MW; CAC3B6D9A3DE2ACE CRC64;
MHGGNHWGGS FEIHGDSATD DERSRNMDID RAALSHHSHS QNLDETQQSW LLGPSEPKKN
KYIDLGCVVC SRKAFRYSVW TVVIALVVIV LPIVIVKSLP KHHPKPPKPD NYTLALHKAL
MFFNAQKSGR LPRSNGVPWR HDSGLQDGSD AKDVKGGLVG GYYDAGDNIK FGFPMAFSMS
MLGWTVVEYA HKYKAIGEYD HVRELIRWGT DYILQTFNSS SNNINHIYSQ VGSARNGSTT
PDDHYCWERP EDMDYPRRSL ASSSAPDLGA EMAAALSAAS IVFRDDPVYS ERLSHAAETV
FKFARDQGHR NRYSAGNPDI EPFYNSTDYF DEHMWGAAWL YYATGNSSYL ALATNPGIAR
NAHNADGGPD KGVLSWDNKI PAAQLLLLRL RMFLNPGYPY EEMLKSYSNI TGLYMCSYLK
RFNVFNFTQG GLIQLNHGRP QTLQYAANAA FLANLYADYM NATGVPGWFC GPYYFSAIVL
RQFAQSQINY ILGDNPRKMS YVVGYGSNYP KHVHHRGASI PNNQKTTCTG GWKWRDSNNP
NPNTITGAMV GGPDRFDQFR DVRSNYNYTE PTLAGNAGLV AALISLTGSG GGGIDTNKIF
SGIPPLFPVS PPPPPPWKP
//
