ID U5DIU0_AMBTC Unreviewed; 725 AA.
AC U5DIU0;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 24-JAN-2024, entry version 52.
DE RecName: Full=Vesicle-fusing ATPase {ECO:0000256|RuleBase:RU367045};
DE EC=3.6.4.6 {ECO:0000256|RuleBase:RU367045};
GN ORFNames=AMTR_s00068p00183110 {ECO:0000313|EMBL:ERN20508.1};
OS Amborella trichopoda.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Amborellales; Amborellaceae; Amborella.
OX NCBI_TaxID=13333 {ECO:0000313|EMBL:ERN20508.1, ECO:0000313|Proteomes:UP000017836};
RN [1] {ECO:0000313|Proteomes:UP000017836}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24357323;
RG Amborella Genome Project;
RT "The Amborella genome and the evolution of flowering plants.";
RL Science 342:1241089-1241089(2013).
CC -!- FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion
CC of transport vesicles within the Golgi cisternae. Is also required for
CC transport from the endoplasmic reticulum to the Golgi stack. Seems to
CC function as a fusion protein required for the delivery of cargo
CC proteins to all compartments of the Golgi stack independent of vesicle
CC origin. {ECO:0000256|RuleBase:RU367045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.6;
CC Evidence={ECO:0000256|RuleBase:RU367045};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU367045};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|RuleBase:RU367045};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU367045}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family.
CC {ECO:0000256|ARBA:ARBA00006914, ECO:0000256|RuleBase:RU367045}.
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DR EMBL; KI392059; ERN20508.1; -; Genomic_DNA.
DR AlphaFoldDB; U5DIU0; -.
DR STRING; 13333.U5DIU0; -.
DR EnsemblPlants; ERN20508; ERN20508; AMTR_s00068p00183110.
DR Gramene; ERN20508; ERN20508; AMTR_s00068p00183110.
DR eggNOG; KOG0741; Eukaryota.
DR HOGENOM; CLU_008037_2_0_1; -.
DR OMA; CFDNEIA; -.
DR Proteomes; UP000017836; Unassembled WGS sequence.
DR GO; GO:0005795; C:Golgi stack; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0007030; P:Golgi organization; IEA:EnsemblPlants.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:EnsemblPlants.
DR GO; GO:1990019; P:protein storage vacuole organization; IEA:EnsemblPlants.
DR GO; GO:0035494; P:SNARE complex disassembly; IEA:InterPro.
DR CDD; cd19504; RecA-like_NSF-SEC18_r1-like; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.10.330.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR029067; CDC48_domain_2-like_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039812; Vesicle-fus_ATPase.
DR PANTHER; PTHR23078:SF3; VESICLE-FUSING ATPASE; 1.
DR PANTHER; PTHR23078; VESICULAR-FUSION PROTEIN NSF; 1.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF54585; Cdc48 domain 2-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00674; AAA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU367045};
KW Cytoplasm {ECO:0000256|RuleBase:RU367045};
KW ER-Golgi transport {ECO:0000256|RuleBase:RU367045};
KW Hydrolase {ECO:0000256|RuleBase:RU367045};
KW Magnesium {ECO:0000256|RuleBase:RU367045};
KW Metal-binding {ECO:0000256|RuleBase:RU367045};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU367045};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW ECO:0000256|RuleBase:RU367045};
KW Reference proteome {ECO:0000313|Proteomes:UP000017836};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU367045}.
FT DOMAIN 232..379
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 512..650
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
SQ SEQUENCE 725 AA; 80116 MW; 9FB417C690325028 CRC64;
MAGRYAHGSS DSIEMQVITT PSTDLALTNL AYCSHWDLRR FDLNRSGYAL VSFRDSIVLT
NVKVSVDDPI HVTRFVPPED FNLALLTVDL DFVKAKGKSE EIDAVQLSQQ LRKRCLYQVM
TVGQRVLFEY HGTNYIFTVN QAVIEGQEES ETFERGMISP ETYIVFECSP NTGIKIVNQR
EAANSKIFKQ KDFNLLSLGI GGLSAEFTDI FRRAFASRVF PAHVTNKLGI KHVKGMLLYG
PPGTGKTLMA RQIGKMLNGR EPKIVNGPEV LSKFVGETEK NVRDLFADAE NDQRTRGDQS
DLHVIIFDEI DAICKSRGST RDGTGVHDSI VNQLLTKIDG VESLNNVLLI GMTNRKDLLD
EALLRPGRLE VQVEISLPDE NGRLQILQIH TNKMKENSFL APDVSLQELA ARTKNYSGAE
LEGVVKSAVS FALNRQISLD DLTKPLHEEN IKVTMDDFLN ALHEITPAFG ASTDNLERCR
LNGMADCGGR HKHIYKRAML LVEQVKVSKG SPLVTCLLEG PGGSGKTAMA ATIGLDSDFA
YVKIISAETM IGLSEASKCA QIVKVFEDAY KSQLSIIILD DIERLLEYVP IGPRFSNLIS
QTLMVLLKRL PPKGKNVLVI GTTSEVGFLE SLGICDAFSV TYHVPTLHTE DAKKVLAALN
VFSEDDIDAA AEALNNMPIK KLYMLVEMAA QGEKGGNSEA IYSGREKINI NHFFDCLGDI
VSVYR
//
