ID U5H9A3_USTV1 Unreviewed; 1267 AA.
AC U5H9A3;
DT 11-DEC-2013, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2013, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN ORFNames=MVLG_03795 {ECO:0000313|EMBL:KDE05852.1};
OS Microbotryum lychnidis-dioicae (strain p1A1 Lamole / MvSl-1064) (Anther
OS smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Microbotryomycetes; Microbotryales; Microbotryaceae; Microbotryum.
OX NCBI_TaxID=683840 {ECO:0000313|EMBL:KDE05852.1};
RN [1] {ECO:0000313|Proteomes:UP000017200}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=p1A1 Lamole {ECO:0000313|Proteomes:UP000017200};
RA Cuomo C., Perlin M., Young S.K., Zeng Q., Gargeya S., Alvarado L.,
RA Berlin A., Chapman S.B., Chen Z., Freedman E., Gellesch M., Goldberg J.,
RA Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Mehta T.,
RA Neiman D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P.,
RA Stolte C., Sykes S., White J., Yandava C., Haas B., Nusbaum C., Birren B.;
RT "The genome sequence of Microbotryum violaceum strain p1A1 Lamole.";
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KDE05852.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=P1A1 Lamole {ECO:0000313|EMBL:KDE05852.1};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Butler R., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Brown A., Chapman S.B., Chen Z., Dunbar C.,
RA Freedman E., Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S.,
RA Heilman E., Heiman D., Howarth C., Larson L., Lui A., MacDonald P.J.P.,
RA Mehta T., Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M.,
RA Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S.,
RA White J., Yandava C., Wortman J., Nusbaum C., Birren B.;
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KDE05852.1, ECO:0000313|Proteomes:UP000017200}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=p1A1 Lamole {ECO:0000313|Proteomes:UP000017200}, and P1A1
RC Lamole {ECO:0000313|EMBL:KDE05852.1};
RX PubMed=26076695; DOI=10.1186/s12864-015-1660-8;
RA Perlin M.H., Amselem J., Fontanillas E., Toh S.S., Chen Z., Goldberg J.,
RA Duplessis S., Henrissat B., Young S., Zeng Q., Aguileta G., Petit E.,
RA Badouin H., Andrews J., Razeeq D., Gabaldon T., Quesneville H., Giraud T.,
RA Hood M.E., Schultz D.J., Cuomo C.A.;
RT "Sex and parasites: genomic and transcriptomic analysis of Microbotryum
RT lychnidis-dioicae, the biotrophic and plant-castrating anther smut
RT fungus.";
RL BMC Genomics 16:461-461(2015).
RN [4] {ECO:0000313|EnsemblFungi:MVLG_03795T0}
RP IDENTIFICATION.
RG EnsemblFungi;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; AEIJ01000371; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; GL541680; KDE05852.1; -; Genomic_DNA.
DR AlphaFoldDB; U5H9A3; -.
DR EnsemblFungi; MVLG_03795T0; MVLG_03795T0; MVLG_03795.
DR HOGENOM; CLU_001871_1_0_1; -.
DR InParanoid; U5H9A3; -.
DR OMA; SWKVHKG; -.
DR OrthoDB; 373802at2759; -.
DR Proteomes; UP000017200; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF7; PUTATIVE (AFU_ORTHOLOGUE AFUA_6G02710)-RELATED; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 4: Predicted;
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000017200};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 898..1062
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 43..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..744
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 480..512
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 600..620
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..697
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 710..738
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1267 AA; 137013 MW; F118B34ABFB2056E CRC64;
MFPSSLSSAD PTTGDPAPRS TPRSTPRTRT DSFAMTAANA LGPCPFSSSS SPPNSIMTGS
RRRSVSAAMD NLPLDAPPSL LIQSEILARL AHGPTLLPSS LAHLISAISV CSRVSIRSAA
LFIEAILESI RQGTTAGLGL ARRALISAVA SARTLHYVAK GLDWDGRNAQ GQRDLGLYLG
LLDRYTNLGI YVINHTFTLA ELFSLAGLSL TMSTTSLAFA TAEESVRMLD SLLGSGETSR
ALSAIIALVR TEMAEDPNFK IIDDGVISTL ALLTRTLTAF VCLQAATSKR TARQMKMRVV
YDCTVVAEGE EMITGCVPFP TTTQGSADEP SSAPALEDHY VREHRRSRAS SIELVAQEFR
RDPHGSLLGS TSPKLMRSRT KSIGHEEIDV QRNEREEETE IVEELNELCG LDQDQMAFGS
DQVDADDALV RLPPEVSAAL EELKHSAAGK TIVTSSMGSH YEVEVTETTT TTTTTVRATA
STMGGDGYST RTLSKRTAPR TRPLSSIFQI AVPQASDVEE GDVSMDDAGD PSPGNSSDEW
VDVGEQSPKQ QPDDVFGPEE SVAAGPSGEG SRNAKLQVVF RTMTSKFTQR KRTFRRVDRN
ETVTTTSTST LSSGESTPRP RRIAVSSEIE EPCSPDSAAS RKSPRRILKA LGKALKRNPS
VENVSSSSPP VSSRSSRTVS RESTNSAGST EQVSRLPSTP PKMPGKTPLA QINDTRSATA
SFASSRRPSL SDLATTSRRG SKVPLEPLNL TGAQVPAQIS TSSGPVPKLR RAESMVSIVT
TCKHSQATPM EEPEPKASNF PRQHLVANLQ RFMRYSSAAY GQAFLRILGL GKSRGLDFSF
PNTKSHANSH AFAHHVGIPV DDILIDSYTT PGGGIFDNHR ISPIVTYLAV DHEMKAIVLS
CRGSLGLSDV LVDLVCSYEQ IHVPDGDTMA LYQVHAGMWH AATRLQRGLV HKTIKEALEQ
YPSYGLVLTG HSLGGGVSSL LSLLWSTPSH VFERNRRPVD IGRHPPIHTP FVTSYASGLP
AGRPIHCYVF GPPCVASPDL GRYCQGLVTS TTHNNDVVPT LSLGTLRDLK TMAMNLHEDG
GTGTTREIIG RVCGIYQRKI TLATSERLYR GLPGGSEKEI PSLHDVSDEA RLVALTKAEI
SAGRGKNRAL DPAYCDPTLL QESEEPEDVV VNDYLWSVMR TLRADNDNDK LYPPGNVFVV
ENHTVFISSE SRPGQYARRE GRRILLRAVD DVEKRFAEPV FGKTLFMDHS PTGYEENVDL
LARAVLG
//
