ID U6EF09_9EURY Unreviewed; 140 AA.
AC U6EF09;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Crossover junction endodeoxyribonuclease Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE Short=Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
DE EC=3.1.21.10 {ECO:0000256|HAMAP-Rule:MF_01490};
DE AltName: Full=Holliday junction resolvase Hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN Name=hjc {ECO:0000256|HAMAP-Rule:MF_01490};
GN ORFNames=MBMB1_1865 {ECO:0000313|EMBL:CDG65943.1};
OS Methanobacterium sp. MB1.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanobacterium.
OX NCBI_TaxID=1379702 {ECO:0000313|EMBL:CDG65943.1, ECO:0000313|Proteomes:UP000017867};
RN [1] {ECO:0000313|EMBL:CDG65943.1, ECO:0000313|Proteomes:UP000017867}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MB1 {ECO:0000313|Proteomes:UP000017867};
RA Maus I., Wibberg D., Stantscheff R., Cibis K., Eikmeyer F.G., Puehler A.,
RA Koenig H., Schlueter A.;
RT "Methanobacterium sp. Mb1.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: A structure-specific endonuclease that resolves Holliday
CC junction (HJ) intermediates during genetic recombination. Cleaves 4-way
CC DNA junctions introducing paired nicks in opposing strands, leaving a
CC 5'-terminal phosphate and a 3'-terminal hydroxyl group that are
CC subsequently ligated to produce recombinant products.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10;
CC Evidence={ECO:0000256|ARBA:ARBA00029354, ECO:0000256|HAMAP-
CC Rule:MF_01490};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01490};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01490};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01490}.
CC -!- SIMILARITY: Belongs to the Holliday junction resolvase Hjc family.
CC {ECO:0000256|HAMAP-Rule:MF_01490}.
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DR EMBL; HG425166; CDG65943.1; -; Genomic_DNA.
DR AlphaFoldDB; U6EF09; -.
DR STRING; 1379702.MBMB1_1865; -.
DR KEGG; meth:MBMB1_1865; -.
DR PATRIC; fig|1379702.4.peg.1848; -.
DR HOGENOM; CLU_139546_0_0_2; -.
DR BioCyc; MSP1379702:MBMB1_RS10305-MONOMER; -.
DR Proteomes; UP000017867; Chromosome.
DR GO; GO:0008821; F:crossover junction DNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_01490; HJ_Resolv_Hjc; 1.
DR InterPro; IPR002732; Hjc.
DR InterPro; IPR014428; Hjc_arc.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR NCBIfam; NF040854; Hol_resolv_Hjc; 1.
DR PANTHER; PTHR39651; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR PANTHER; PTHR39651:SF1; HOLLIDAY JUNCTION RESOLVASE HJC; 1.
DR Pfam; PF01870; Hjc; 1.
DR PIRSF; PIRSF004985; Hlld_jn_rslvs_ar; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_01490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_01490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01490};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_01490};
KW Reference proteome {ECO:0000313|Proteomes:UP000017867}.
FT ACT_SITE 36
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 16
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 42
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT BINDING 55
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
FT SITE 57
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01490"
SQ SEQUENCE 140 AA; 16031 MW; 143FD602106BB80B CRC64;
MMAKVQEIKA EGIEHERNLV NSLKRSGYVA GRLQSSGVSL PDVIAGDGES IFVFEVKSTA
TSNIKIYKNQ IWTLKKFAYN FKAKPYIAVY FTGRMMDFLF IKPRDLKEYD KMFTIDYNTA
CLKGKDLGEI ISNELQKRLI
//
