ID U6F4Q3_LACHE Unreviewed; 387 AA.
AC U6F4Q3;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=Hydroxymethylglutaryl-CoA synthase {ECO:0000313|EMBL:CDI58248.1};
DE EC=2.3.3.10 {ECO:0000313|EMBL:CDI58248.1};
GN ORFNames=LHCIRMBIA951_00461 {ECO:0000313|EMBL:CDI58248.1};
OS Lactobacillus helveticus CIRM-BIA 951.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=1226334 {ECO:0000313|EMBL:CDI58248.1, ECO:0000313|Proteomes:UP000017248};
RN [1] {ECO:0000313|EMBL:CDI58248.1, ECO:0000313|Proteomes:UP000017248}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CIRM-BIA 951 {ECO:0000313|EMBL:CDI58248.1,
RC ECO:0000313|Proteomes:UP000017248};
RA Valence F., Chuat V., Ma L., Creno S., Falentin H., Lortal S., Bizet C.,
RA Clermont D., Loux V., Bouchier C., Cousin S.;
RT "Draft Genome Sequence of five Lactobacillus helveticus strains CIRM-BIA
RT 101T, 103, 104, 951 and 953 isolated from milk product.";
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. HMG-CoA synthase
CC family. {ECO:0000256|ARBA:ARBA00007061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDI58248.1}.
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DR EMBL; CBUK010000059; CDI58248.1; -; Genomic_DNA.
DR RefSeq; WP_023190743.1; NZ_HG530817.1.
DR AlphaFoldDB; U6F4Q3; -.
DR STRING; 1587.ALV80_07990; -.
DR HOGENOM; CLU_008065_3_2_9; -.
DR Proteomes; UP000017248; Unassembled WGS sequence.
DR GO; GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR GO; GO:0010142; P:farnesyl diphosphate biosynthetic process, mevalonate pathway; IEA:InterPro.
DR CDD; cd00827; init_cond_enzymes; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR013746; HMG_CoA_synt_C_dom.
DR InterPro; IPR013528; HMG_CoA_synth_N.
DR InterPro; IPR011554; HMG_CoA_synthase_prok.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01835; HMG-CoA-S_prok; 1.
DR PANTHER; PTHR43323; 3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE; 1.
DR PANTHER; PTHR43323:SF2; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR Pfam; PF08540; HMG_CoA_synt_C; 2.
DR Pfam; PF01154; HMG_CoA_synt_N; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:CDI58248.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000017248};
KW Transferase {ECO:0000313|EMBL:CDI58248.1}.
FT DOMAIN 3..164
FT /note="Hydroxymethylglutaryl-coenzyme A synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01154"
FT DOMAIN 176..243
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT DOMAIN 254..356
FT /note="Hydroxymethylglutaryl-coenzyme A synthase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08540"
FT ACT_SITE 79
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 111
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT ACT_SITE 233
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-1"
FT BINDING 29
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 143
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 242
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
FT BINDING 275
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR611554-2"
SQ SEQUENCE 387 AA; 42746 MW; 7CB4B22A8E9F983B CRC64;
MRVGIDKIGL FTPNKYVDMV DLAHARNEDP NKYLIGIGQS EMSVADQTQD AVSMGINATM
KYIDRIDKDK IGLLVFGTES GIDQSKSASL FVKTALKLKP EVRTFEVKEA CFGLTAALMI
ARDFVRVHPD QTAMVIGSDI ARYGIGTAGE VTQGAGSVSM LIKADPAILA LNDGHSAYSE
DINDFWRPND SKLAMVDGKY STQVYLDFFS KTFADYKKQK KLETNDFDAI IYHLPFTKMG
LKANLLAVAD QDEATTEKLQ NSFDASKQLS RRVGNIYTAS LYMSLLSLLE NGNLPAGALV
GLFSYGSGAM GEFYSGNLVE GYEKEVNAAG DEKMLKRRQK VSVPEYEEIF NTALDDPEDN
EELTSDDEKG TWYFAGTKGN IRQYKVK
//
