ID   U6LSD0_9EIME            Unreviewed;       887 AA.
AC   U6LSD0;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   24-JAN-2024, entry version 26.
DE   SubName: Full=Insulysin, putative {ECO:0000313|EMBL:CDJ50715.1};
GN   ORFNames=EBH_0086180 {ECO:0000313|EMBL:CDJ50715.1};
OS   Eimeria brunetti.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC   Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria.
OX   NCBI_TaxID=51314 {ECO:0000313|EMBL:CDJ50715.1};
RN   [1] {ECO:0000313|EMBL:CDJ50715.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Houghton {ECO:0000313|EMBL:CDJ50715.1};
RA   Reid A.J., Blake D., Billington K., Browne H., Dunn M., Hung S.,
RA   Kawahara F., Miranda-Saavedra D., Mourier T., Nagra H., Otto T.D.,
RA   Rawlings N., Sanchez A., Sanders M., Subramaniam C., Tay Y., Dear P.,
RA   Doerig C., Gruber A., Parkinson J., Shirley M., Wan K.L., Berriman M.,
RA   Tomley F., Pain A.;
RT   "Genomic analysis of the causative agents of coccidiosis in chickens.";
RL   Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:CDJ50715.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Houghton {ECO:0000313|EMBL:CDJ50715.1};
RA   Aslett M.;
RL   Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
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DR   EMBL; HG712375; CDJ50715.1; -; Genomic_DNA.
DR   AlphaFoldDB; U6LSD0; -.
DR   EnsemblProtists; CDJ50715; CDJ50715; EBH_0086180.
DR   VEuPathDB; ToxoDB:EBH_0086180; -.
DR   OrthoDB; 129328at2759; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 1.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670}; Signal {ECO:0000256|SAM:SignalP};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..887
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004672812"
FT   DOMAIN          46..168
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          257..542
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          562..722
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   887 AA;  98543 MW;  42A943F2F915C87E CRC64;
     MRNAASVGLC VGLSAIGALA NDILKPEADY RDFRHYQLSN GMHAIAVHHP RSNEAGFAVA
     ANTGSLYDPE DVPGLAHFLE HMLFLGTSKY PEPESYDGFL TQNGGANNAY TDEEKTVFFN
     KVTDSAFEEA LDRFAEFFKS PLFNRQYEEK EVNAIDAEHQ KNIPNDDERA WYTIRSLAKG
     PMSRFATGNS ETLSTNPKAK GIDLVDRLRD FHSKYYCGSN MEIFSYINFL RDHGVGHDLV
     STMAQQSHID FHTTQPSSSI MDEAARLAHN LLTYEPYHVV AGDSLLIDAD PRLTNQLLQA
     MSPSKAIIAF SDPEFTAKVD NFETDPYYGV QFRVLDLPQH HAVAMAVLTA SPNAFRMPPP
     LMHIPKASEL KILPGLLGLT EPELISEQGG NAGTAVWWQG QGAFAVPRIA VQLNGSVSKD
     KADMLSRAQG SLALAAIAEH LQEETVDFQN CGITHSLAFK GTGFHMTFEG YTQAQLGKLM
     AHVANLLSDP SMVEPERFER IKQKQMKLLA DPATSMAFEH ALEAAAILTR NDAFSRKDLL
     NALQQTNYDD SITKLSELKN VHVDAFVMGN IGRDQSLAMV EDFLEQAGFT PIDHDDAVAS
     LAMEQKQTIE ATLANPIRGD KDHASLVQFQ LGIPSIEDRV NLAVLTQFLN RRIYDSLRTE
     AQLGYIAGAK ESQAASTALL QCFVEGAKTH PDEVVKMIDE ELSKAKEYLA NMPDAEMARW
     KEAAHAKLTK MEANFSEDFK KSAGEIFSHS NCFTKKELEV KYLDNDFSRK QLLRTFAKLS
     DPSRRMVVKL VANLEPEKEV TLIGESRAQD AALLRRGDTG KRILDYCFHE CYNEVSVAEE
     IKAPTKLLSL EDKFVSQVQE ANGYFPDVSV CELPPSLLSL FELLEDL
//