ID U6N4N5_9EIME Unreviewed; 1187 AA.
AC U6N4N5;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 08-NOV-2023, entry version 29.
DE RecName: Full=DNA ligase (NAD(+)) {ECO:0000256|ARBA:ARBA00012722};
DE EC=6.5.1.2 {ECO:0000256|ARBA:ARBA00012722};
GN ORFNames=ENH_00080810 {ECO:0000313|EMBL:CDJ70254.1};
OS Eimeria necatrix.
OC Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia;
OC Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria.
OX NCBI_TaxID=51315 {ECO:0000313|EMBL:CDJ70254.1};
RN [1] {ECO:0000313|EMBL:CDJ70254.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Houghton {ECO:0000313|EMBL:CDJ70254.1};
RA Reid A.J., Blake D., Billington K., Browne H., Dunn M., Hung S.,
RA Kawahara F., Miranda-Saavedra D., Mourier T., Nagra H., Otto T.D.,
RA Rawlings N., Sanchez A., Sanders M., Subramaniam C., Tay Y., Dear P.,
RA Doerig C., Gruber A., Parkinson J., Shirley M., Wan K.L., Berriman M.,
RA Tomley F., Pain A.;
RT "Genomic analysis of the causative agents of coccidiosis in chickens.";
RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CDJ70254.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Houghton {ECO:0000313|EMBL:CDJ70254.1};
RA Aslett M.;
RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC nicotinamide D-nucleotide.; EC=6.5.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00034005};
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DR EMBL; HG725903; CDJ70254.1; -; Genomic_DNA.
DR RefSeq; XP_013438720.1; XM_013583266.1.
DR AlphaFoldDB; U6N4N5; -.
DR GeneID; 25478210; -.
DR VEuPathDB; ToxoDB:ENH_00080810; -.
DR OrthoDB; 11121at2759; -.
DR GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1490.70; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 1.10.287.610; Helix hairpin bin; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR013839; DNAligase_adenylation.
DR InterPro; IPR013840; DNAligase_N.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004150; NAD_DNA_ligase_OB.
DR InterPro; IPR010994; RuvA_2-like.
DR Pfam; PF01653; DNA_ligase_aden; 2.
DR Pfam; PF03120; DNA_ligase_OB; 1.
DR SMART; SM00532; LIGANc; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 3.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF47781; RuvA domain 2-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:CDJ70254.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..1187
FT /note="DNA ligase (NAD(+))"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004674330"
FT DOMAIN 203..858
FT /note="NAD-dependent DNA ligase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00532"
FT REGION 1060..1083
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1187 AA; 125275 MW; D53A5C261E2B96FC CRC64;
MQDSLASRVY HLSQMLLLLH CSKVVATAIQ HPSCPAESAE HKLQRLILPA SCCLRARSIE
AASAAFLDMR TPAFRNSGAS TTFSCSRPFQ TIAEQQLPKQ QRNQPSLANG FPITTVMTCL
RWHGLQSMLR SNISSKTISG NWRGSKAIST RYSYGAPVFG GAAQNRRNTE DVAASTVAAN
APVATNATGE AVAPAVAAPG KAAAAAAHTL LCSKINELNK AYYSGEEPLA SDEEFDALWL
RLGELEKKYP GLGGPSSPLR RIGHGTLGTA TAAGNLQHVS PVLSLESVHS PGEANTLFDR
LDRFVAKALH GVLRTSVGRS RRRKCILKAA AETNTAGILY ESVAAAPATA AVAEATTTVP
AATDAAAGGD VFAEALLMEP KVDGQSVSLT YELSSSNTNS GDNTGGGILR YRLVRALTRG
DGRRGESITQ KALQMAHTGA LPLVIDVPST KETRRATSAS PTTAVAAAPS AAQGVFGMPR
TLEVRGEAYM CLSALSALNK RRMMEGLKPH AVVGMLRRLD EEANCAIRFC AYALVGASDS
ALSGHQNDHQ DTHRASISAV AAAASAAGNA VAGVTTQLEL LQRLQQWGFE VLLPHCPLVV
GRKDALKAFS EQSRCHEIYM SNLGALHAAT AGAPHANGAS RHHGCNADSA PLGGGAINPA
SVGGGVTNSA LFGDGTSYVS GAARERSAGA SSPRTAGASL AADCSFQLED IPCDGVVFKV
NSLPLHQRLG STARAPRWAF ALKFAAKVSK TKVVGIEWTV GSSGVCTPVA LLQPVLLGGV
LVARASLHSP QEVRRKDVRV GDWVHLQLKG ESVPHIVAVD LEDREEGAEP ALLPSACPSC
GRGLIERSSP TGKLSKRCKQ RLEAKDRSDR RSEECSAAKV AVAAPAASSV RTFVGEEAVT
GEGEGGRGDK AFGVLWCPGG WACASQRLLR LRRFFSREGV AVAGLGPRAL EALISRGYLE
APSDAFHLAQ VDRQRTAAGE SGIAGWPGWG PQARDALFKE IEQVQGRGVP LQQLLFALGI
PGMGRRAAAA VSHHVRTIGG FLALLDNLSA SCPIDRILQN GQTPQETPKL TASPQTSQDS
QHPFGCKTML HTPQNIPIPQ GSEDLQMLED HQVSRDVDGL ESRATLRGLH SPTGDRSEQS
LDWTLALPGI ELSLVKELRM FANDKRNRQQ LIEVTKVLPV HPETTAG
//
