ID U7ULJ1_9FIRM Unreviewed; 590 AA.
AC U7ULJ1;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=B12 binding domain protein {ECO:0000313|EMBL:ERT60292.1};
GN ORFNames=HMPREF1250_0957 {ECO:0000313|EMBL:ERT60292.1};
OS Megasphaera vaginalis.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Megasphaera.
OX NCBI_TaxID=1111454 {ECO:0000313|EMBL:ERT60292.1, ECO:0000313|Proteomes:UP000017090};
RN [1] {ECO:0000313|EMBL:ERT60292.1, ECO:0000313|Proteomes:UP000017090}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BV3C16-1 {ECO:0000313|EMBL:ERT60292.1,
RC ECO:0000313|Proteomes:UP000017090};
RA Durkin A.S., Haft D.R., McCorrison J., Torralba M., Gillis M., Haft D.H.,
RA Methe B., Sutton G., Nelson K.E.;
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERT60292.1}.
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DR EMBL; AWXA01000025; ERT60292.1; -; Genomic_DNA.
DR AlphaFoldDB; U7ULJ1; -.
DR STRING; 1111454.HMPREF1250_0957; -.
DR PATRIC; fig|1111454.3.peg.939; -.
DR eggNOG; COG1032; Bacteria.
DR Proteomes; UP000017090; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000017090}.
FT DOMAIN 2..136
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 176..404
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 590 AA; 67068 MW; A133008CC8F6C022 CRC64;
MMKILLTTLN AKFVQSSLAL RCLAAACRQQ GFGNVETAEY TINHNAYDIL RRIHAFHADV
IGFSCYIWNI DMTLHLISLL RQICPDVTIM VGGPEVTYTA RELLTAEGNI DYALQGEGEA
ILPELIASWL NGEDGTAVGG VLRRTADGAI EGNELIREVP DLDLLPFPYD DADLASLSHQ
LVYYESSRGC PFHCQYCLSG RDDTVRFRSA ALVIEELKRL VAAGVKQVKL VDRTFNCNPR
HYRPILAFMV SLQATVNFHL EIEPGLVTEE DLALLSQAPR GRIQLELGIQ STYGPTLQAI
RRHNDWPHIV HVMNVIRSLG TIHLHLDLIV GLPYETMQAL RRSFNDIYSL QPHKLQIGFL
KLLKGSGIRR DYSQDYACDL RGPYEVLATK WLSYEAVGTM KVFEDVFERI YNSGKFSYTL
AYVDRFFAGD WFSFYERLTD HWLCRGFDRL AVSDATVCHF LWDSLTALTE LTAEEKDVAR
QLLCLDMLTC FRFRLKPSFL GWEEAPRQLT DPFFRREEGP AAYFTGYRFT NWRDIKMRYH
LWPITPAVGK ELRRYADGPD EAAYILAESG ADGVCWRLLN KAALAAEGVI
//
