ID URE32_BRUA2 Reviewed; 100 AA.
AC Q2YQE0;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=Urease subunit gamma 2 {ECO:0000255|HAMAP-Rule:MF_00739};
DE EC=3.5.1.5 {ECO:0000255|HAMAP-Rule:MF_00739};
DE AltName: Full=Urea amidohydrolase subunit gamma 2 {ECO:0000255|HAMAP-Rule:MF_00739};
GN Name=ureA2 {ECO:0000255|HAMAP-Rule:MF_00739}; OrderedLocusNames=BAB1_1376;
OS Brucella abortus (strain 2308).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
RN [2]
RP LACK OF ROLE IN VIRULENCE.
RX PubMed=17101645; DOI=10.1128/iai.01244-06;
RA Sangari F.J., Seoane A., Rodriguez M.C., Aguero J., Garcia Lobo J.M.;
RT "Characterization of the urease operon of Brucella abortus and assessment
RT of its role in virulence of the bacterium.";
RL Infect. Immun. 75:774-780(2007).
CC -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity
CC or pathogen survival in BALB/c mice when administered orally.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00739};
CC -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC subunits. Three heterotrimers associate to form the active enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- SIMILARITY: Belongs to the urease gamma subunit family.
CC {ECO:0000255|HAMAP-Rule:MF_00739}.
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DR EMBL; AM040264; CAJ11332.1; -; Genomic_DNA.
DR RefSeq; WP_002964469.1; NZ_KN046823.1.
DR PDB; 4FUR; X-ray; 2.10 A; A/B/C/D/E/F=1-100.
DR PDBsum; 4FUR; -.
DR AlphaFoldDB; Q2YQE0; -.
DR SMR; Q2YQE0; -.
DR STRING; 359391.BAB1_1376; -.
DR GeneID; 58775583; -.
DR KEGG; bmf:BAB1_1376; -.
DR PATRIC; fig|359391.11.peg.826; -.
DR HOGENOM; CLU_145825_1_0_5; -.
DR UniPathway; UPA00258; UER00370.
DR Proteomes; UP000002719; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00390; Urease_gamma; 1.
DR Gene3D; 3.30.280.10; Urease, gamma-like subunit; 1.
DR HAMAP; MF_00739; Urease_gamma; 1.
DR InterPro; IPR012010; Urease_gamma.
DR InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR InterPro; IPR036463; Urease_gamma_sf.
DR NCBIfam; TIGR00193; urease_gam; 1.
DR PANTHER; PTHR33569; UREASE; 1.
DR PANTHER; PTHR33569:SF1; UREASE; 1.
DR Pfam; PF00547; Urease_gamma; 1.
DR PIRSF; PIRSF001223; Urease_gamma; 1.
DR SUPFAM; SSF54111; Urease, gamma-subunit; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..100
FT /note="Urease subunit gamma 2"
FT /id="PRO_0000234200"
FT HELIX 5..25
FT /evidence="ECO:0007829|PDB:4FUR"
FT HELIX 32..48
FT /evidence="ECO:0007829|PDB:4FUR"
FT HELIX 53..60
FT /evidence="ECO:0007829|PDB:4FUR"
FT TURN 61..63
FT /evidence="ECO:0007829|PDB:4FUR"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:4FUR"
FT HELIX 73..76
FT /evidence="ECO:0007829|PDB:4FUR"
FT STRAND 78..86
FT /evidence="ECO:0007829|PDB:4FUR"
FT STRAND 89..97
FT /evidence="ECO:0007829|PDB:4FUR"
SQ SEQUENCE 100 AA; 10943 MW; FABF969BACC53D05 CRC64;
MHLTPREFDK LVIHMLSDVA LKRKNKGLKL NHPEAVAVLS AYVLDGAREG KTVEEVMDGA
RSVLKADDVM DGVPDLLPLI QVEAVFSDGS RLVSLHNPIT
//
