ID UVRA_HAEIN Reviewed; 943 AA.
AC P44410; Q48151;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 27-MAR-2024, entry version 152.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=HI_0249;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NTHi N187;
RX PubMed=8921840; DOI=10.1016/0378-1119(96)00264-8;
RA de la Morena M.L., Hendrixson D.R., St Geme J.W. III;
RT "Isolation and characterization of the Haemophilus influenzae uvrA gene.";
RL Gene 177:23-28(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71.
RC STRAIN=NTHi TN106;
RX PubMed=8063092; DOI=10.1016/0378-1119(94)90841-9;
RA Jarosik G.P., Hansen E.J.;
RT "Cloning and sequencing of the Haemophilus influenzae ssb gene encoding
RT single-strand DNA-binding protein.";
RL Gene 146:101-103(1994).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; L42023; AAC21915.1; -; Genomic_DNA.
DR EMBL; U33877; AAC44592.1; -; Genomic_DNA.
DR EMBL; U04997; AAA60462.1; -; Genomic_DNA.
DR PIR; D64057; D64057.
DR RefSeq; NP_438418.1; NC_000907.1.
DR AlphaFoldDB; P44410; -.
DR SMR; P44410; -.
DR STRING; 71421.HI_0249; -.
DR EnsemblBacteria; AAC21915; AAC21915; HI_0249.
DR KEGG; hin:HI_0249; -.
DR PATRIC; fig|71421.8.peg.264; -.
DR eggNOG; COG0178; Bacteria.
DR HOGENOM; CLU_001370_0_2_6; -.
DR OrthoDB; 9809851at2; -.
DR PhylomeDB; P44410; -.
DR BioCyc; HINF71421:G1GJ1-263-MONOMER; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR CDD; cd03270; ABC_UvrA_I; 1.
DR CDD; cd03271; ABC_UvrA_II; 1.
DR Gene3D; 3.30.190.20; -; 1.
DR Gene3D; 1.10.8.280; ABC transporter ATPase domain-like; 1.
DR Gene3D; 1.20.1580.10; ABC transporter ATPase like domain; 3.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR NCBIfam; TIGR00630; uvra; 1.
DR PANTHER; PTHR43152; UVRABC SYSTEM PROTEIN A; 1.
DR PANTHER; PTHR43152:SF3; UVRABC SYSTEM PROTEIN A; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..943
FT /note="UvrABC system protein A"
FT /id="PRO_0000093053"
FT DOMAIN 310..587
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 607..937
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 253..280
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 740..766
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 640..647
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT CONFLICT 163
FT /note="V -> L (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="E -> D (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 425
FT /note="R -> K (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="I -> M (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 514
FT /note="E -> Q (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 661
FT /note="A -> T (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 928
FT /note="T -> E (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
FT CONFLICT 935..942
FT /note="FLKPILEK -> PL (in Ref. 2; AAC44592)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 943 AA; 104366 MW; 4DBA0DCFA602D465 CRC64;
MENIDIRGAR THNLKNINLT IPRNKLVVIT GLSGSGKSSL AFDTLYAEGQ RRYVESLSAY
ARQFLSLMEK PDVDSIEGLS PAISIEQKST SHNPRSTVGT ITEIYDYLRL LFARVGEPRC
PDHNVPLTAQ TISQMVDKVL SLPEDSKMML LAPVVKNRKG EHVKILENIA AQGYIRARID
GEICDLSDPP KLALQKKHTI EVVVDRFKVR SDLATRLAES FETALELSGG TAIVAEMDNP
KAEELVFSAN FACPHCGYSV PELEPRLFSF NNPAGACPTC DGLGVQQYFD EDRVVQNPTI
SLAGGAVKGW DRRNFYYYQM LTSLAKHYHF DVEAPYESLP KKIQHIIMHG SGKEEIEFQY
MNDRGDVVIR KHPFEGILNN MARRYKETES MSVREELAKN ISNRPCIDCG GSRLRPEARN
VYIGRTNLPI IAEKSIGETL EFFTALSLTG QKAQIAEKIL KEIRERLQFL VNVGLNYLSL
SRSAETLSGG EAQRIRLASQ IGAGLVGVMY VLDEPSIGLH QRDNERLLNT LIHLRNLGNT
VIVVEHDEDA IRAADHIIDI GPGAGVHGGQ VIAQGNADEI MLNPNSITGK FLSGADKIEI
PKKRTALDKK KWLKLKGASG NNLKNVNLDI PVGLFTCVTG VSGSGKSTLI NDTLFPLAQN
ALNRAEKTDY APYQSIEGLE HFDKVIDINQ SPIGRTPRSN PATYTGLFTP IRELFAGVPE
ARARGYNPGR FSFNVRGGRC EACQGDGVLK VEMHFLPDVY VPCDQCKGKR YNRETLEIRY
KGKTIHQVLD MTVEEAREFF DAIPMIARKL QTLMDVGLSY IRLGQSSTTL SGGEAQRVKL
ATELSKRDTG KTLYILDEPT TGLHFADIKQ LLEVLHRLRD QGNTIVVIEH NLDVIKTADW
IVDLGPEGGS GGGQIIATGT PEQVAKVTSS HTARFLKPIL EKP
//
