ID UVRA_LACPL Reviewed; 951 AA.
AC Q88YI7; F9ULZ7;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 121.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=lp_0773;
OS Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS (Lactobacillus plantarum).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactiplantibacillus.
OX NCBI_TaxID=220668;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA Siezen R.J.;
RT "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX PubMed=22156394; DOI=10.1128/jb.06275-11;
RA Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA Kleerebezem M., van Hijum S.A.;
RT "Complete resequencing and reannotation of the Lactobacillus plantarum
RT WCFS1 genome.";
RL J. Bacteriol. 194:195-196(2012).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; AL935263; CCC78236.1; -; Genomic_DNA.
DR RefSeq; WP_003643969.1; NC_004567.2.
DR RefSeq; YP_004888750.1; NC_004567.2.
DR AlphaFoldDB; Q88YI7; -.
DR SMR; Q88YI7; -.
DR STRING; 220668.lp_0773; -.
DR EnsemblBacteria; CCC78236; CCC78236; lp_0773.
DR GeneID; 77217297; -.
DR KEGG; lpl:lp_0773; -.
DR PATRIC; fig|220668.9.peg.653; -.
DR eggNOG; COG0178; Bacteria.
DR HOGENOM; CLU_001370_0_2_9; -.
DR OrthoDB; 9809851at2; -.
DR PhylomeDB; Q88YI7; -.
DR Proteomes; UP000000432; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR CDD; cd03270; ABC_UvrA_I; 1.
DR CDD; cd03271; ABC_UvrA_II; 1.
DR Gene3D; 1.10.8.280; ABC transporter ATPase domain-like; 1.
DR Gene3D; 1.20.1580.10; ABC transporter ATPase like domain; 2.
DR Gene3D; 3.30.1490.20; ATP-grasp fold, A domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR NCBIfam; TIGR00630; uvra; 1.
DR PANTHER; PTHR43152; UVRABC SYSTEM PROTEIN A; 1.
DR PANTHER; PTHR43152:SF3; UVRABC SYSTEM PROTEIN A; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..951
FT /note="UvrABC system protein A"
FT /id="PRO_0000093057"
FT DOMAIN 309..587
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 607..935
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 252..279
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 738..764
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 639..646
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
SQ SEQUENCE 951 AA; 104985 MW; E7C3A91420CBB1DD CRC64;
MANDKIVIHG ARAHNLKDID VTIPRDKLVV ITGLSGSGKS SLAFDTLYAE GQRRYVESLS
AYARQFLGQM QKPDVDSIDG LSPAISIDQK TTSKNPRSTV GTVTEINDYL RLLWARVGEP
ICPNDGTPIA SQTVEQMVDR IQKLPERTKL QILSPIVRQK KGEHKKIFEK IKREGFVRVR
VDGDIHDISE TFELNKNQQH TIEIVIDRIV VKSGDRSRLF DSFEAALRLS GGYAIADVIG
GEPIMFSEHY ACPICGFTVG ELEPRLFSFN APQGACPDCE GLGIKLEVDE DLVVPDKSLT
LAEGALAPWN PISSQYYPEM LKQACEQLEI PMDVPYEDLS KADQQTVLYG SNGKTFHFHY
QNDFGGVRDV DAVFEGVINN VDRRYHETNS DFTRDVMRKY MTELTCQTCH GFRLNRKALS
VKVGGEHIGM VSDLAIGKEL DFFNELSLSE QSLVIAKPIL KEIRDRLSFL QNVGLAYLTL
SRSARTLSGG EAQRIRLATQ IGSNLSGVLY ILDEPSIGLH QRDNDRLIGS LKKMRDLGNT
LIVVEHDEDT MRAADYIVDI GPGAGENGGE VMAAGTPKQV ARSRKSLTGQ YLSGKRFIPL
PETRRPGNGK KIRITGAAEN NLKQIDVDFP LGEFVVVTGV SGSGKSTLVN DVLKRVLAQK
LNRNSEKPGK YKSVSGIKNI ERLVNIDQSP IGRTPRSNPA TYTGVFDNIR DLFAQTNEAK
LRGYKKGRFS FNTKGGRCEA CHGDGILKIE MNFLPDVFVP CEVCHGKQYN SETLEVEYKG
KNIADVLQMT ASEAVKFFEP IPKIRRKLQT LVDVGLGYVK LGQPATTLSG GEAQRMKLAS
ELHKQQSGKN FYILDEPTTG LHSEDIRRLI GVLDRLVDAG NTVLIIEHNL DVVKSADYLI
DLGPEGGDGG GTIVATGTPE QVAEVAESYT GQYLKPVLER DRAREATAPA K
//
