ID V1DBW2_9FIRM Unreviewed; 746 AA.
AC V1DBW2;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=HMPREF9089_00562 {ECO:0000313|EMBL:ESE31139.1};
OS Eubacterium brachy ATCC 33089.
OC Bacteria; Bacillota; Clostridia; Eubacteriales;
OC Eubacteriales Family XIII. Incertae Sedis.
OX NCBI_TaxID=1321814 {ECO:0000313|EMBL:ESE31139.1, ECO:0000313|Proteomes:UP000017536};
RN [1] {ECO:0000313|EMBL:ESE31139.1, ECO:0000313|Proteomes:UP000017536}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33089 {ECO:0000313|EMBL:ESE31139.1,
RC ECO:0000313|Proteomes:UP000017536};
RA Weinstock G., Sodergren E., Wylie T., Fulton L., Fulton R., Fronick C.,
RA O'Laughlin M., Godfrey J., Miner T., Herter B., Appelbaum E., Cordes M.,
RA Lek S., Wollam A., Pepin K.H., Palsikar V.B., Mitreva M., Wilson R.K.;
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESE31139.1}.
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DR EMBL; AXUD01000004; ESE31139.1; -; Genomic_DNA.
DR AlphaFoldDB; V1DBW2; -.
DR STRING; 1321814.HMPREF9089_00562; -.
DR PATRIC; fig|1321814.3.peg.528; -.
DR eggNOG; COG0550; Bacteria.
DR HOGENOM; CLU_002929_5_2_9; -.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000017536; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:ESE31139.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000017536};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 2..143
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 746 AA; 86582 MW; 7D884887C1AC4FAD CRC64;
MKKVIIAEKP SVARNIADAV GIKKKNKGYM EGDDYIITWV FGHLLQLYDA VDYDVNMKSW
KLEKFPFIPQ RFKYKVKKDR KGTEDKGARD QLETIRKLID RDDVDEIISA TDWDREGQII
ADEIFNHIES RKSIKKPIKR ILLNEWTKEE VQKGLRDLKE NCQLSSLSDA GFSRQTADWL
IGINLTSVAT VKYNNSGHKN MLNVGRVLMP TLKIIYDRDK EIERFVSSKY HKLNVQFVTD
EGEKFDATYY EMKRNSKDRE NDDSLNAAEN GEEKYSEKFD DRKYLEDILE KSLNENAVIY
KKEVETKREN PPLLFNLSNL QGYITSKHRG FTADKVLKVA QELYEKKLIT YPRTASNVLD
ESVKDKVKKV LEIHRQDREF KDEINFKDTK RIFDSRKVES HSAIIPTYVK AKGLTDDEKH
VYTAVLNRFL AQFMKQSISE ETKLWIKVEG VEIVGIFIAK GKIQVEEGFK KVEKIQSKDT
LLPIVEEKER AILSAGKVAE VKRKPPKYHT EKTLLRVMET CGKSIGDKDE EESEEMMMSI
LSGFSIGTPA TRAETIKKLK DVGYVVADKK KLKTTELGRM MVEKFPVKEL FDLEYTGRLE
KTLSDIEKKK FTRSEFIDLI TDFTVKSVDN IKRDVVFACD VKVEDSKRVV GICPNCKNPV
LENEKSFSCS NWRNGCKFTI WKEDKYIQSF GKKVSREMVK LLLQNGKVGF HGLTSKKGNK
FSGYFRYVKD ETTDRFKWTL EFIKKS
//
