ID V2Q8A9_9BACT Unreviewed; 567 AA.
AC V2Q8A9;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase {ECO:0000313|EMBL:USF22964.1};
DE EC=2.8.4.3 {ECO:0000313|EMBL:USF22964.1};
GN Name=miaB_1 {ECO:0000313|EMBL:USF22964.1};
GN ORFNames=N508_000016 {ECO:0000313|EMBL:USF22964.1}, N508_01989
GN {ECO:0000313|EMBL:ESJ97186.1};
OS Mucispirillum schaedleri ASF457.
OC Bacteria; Deferribacterota; Deferribacteres; Deferribacterales;
OC Mucispirillaceae; Mucispirillum.
OX NCBI_TaxID=1379858 {ECO:0000313|EMBL:ESJ97186.1};
RN [1] {ECO:0000313|EMBL:ESJ97186.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ASF457 {ECO:0000313|EMBL:ESJ97186.1};
RX PubMed=24723722;
RA Wannemuehler M.J., Overstreet A.M., Ward D.V., Phillips G.J.;
RT "Draft genome sequences of the altered schaedler flora, a defined bacterial
RT community from gnotobiotic mice.";
RL Genome Announc. 2:e00287-e00214(2014).
RN [2] {ECO:0000313|EMBL:USF22964.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ASF457 {ECO:0000313|EMBL:USF22964.1};
RA Proctor A.L., Phillips G.J., Wannemuehler M.J.;
RL Submitted (MAY-2022) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:USF22964.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ASF457 {ECO:0000313|EMBL:USF22964.1};
RA Proctor A., Parvinroo S., Richie T., Jia X., Lee S.T.M., Karp P.D.,
RA Paley S., Kostic A.D., Pierre J.F., Wannemuehler M.J., Phillips G.J.;
RT "Resources to Facilitate Use of the Altered Schaedler Flora (ASF) Mouse
RT Model to Study Microbiome Function.";
RL Submitted (JUN-2022) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AYGZ01000022; ESJ97186.1; -; Genomic_DNA.
DR EMBL; CP097562; USF22964.1; -; Genomic_DNA.
DR RefSeq; WP_023276787.1; NZ_FTRD01000036.1.
DR AlphaFoldDB; V2Q8A9; -.
DR STRING; 1379858.N508_01989; -.
DR GeneID; 78586761; -.
DR PATRIC; fig|1379858.3.peg.1980; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_018288_2_0_0; -.
DR OrthoDB; 9803479at2; -.
DR Proteomes; UP000017429; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR013704; UPF0313_N.
DR NCBIfam; TIGR03904; SAM_YgiQ; 1.
DR PANTHER; PTHR32331; UPF0313 PROTEIN YGIQ; 1.
DR PANTHER; PTHR32331:SF0; UPF0313 PROTEIN YGIQ; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000017429};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000313|EMBL:USF22964.1}.
FT DOMAIN 284..557
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 567 AA; 64587 MW; 3A2DA4E8E97FA8DA CRC64;
MFLPVSMEEV KKLGWDYIDV IIVTGDAYID SPFSGTAVIG KYLLKHGFRV AVISQPDTES
DKDITIFGEP RLFWGINAGC VDSLIANYTA TGKPRKMCDF TPNGINNKRP DRALIVYTGL
IRRFYKNTKP ILLGGIEASL RRIAHYDFKT DKVRRSILFD SKADYIIYGM GELPVLEFAN
ALKNNENVKN IKGLCYISKE LCDNTVILPH YEEVLQDKKL FHKMFKLFSD NSQAHSAKTL
VQKTGDRYLI QNPPVYYNDD ILDEISDIEF ERAVHPKIEG KVKALETIKT SVISHRGCFG
GCSFCAIAVH QGRQVISRSI SSIEKEVNSI VNMKGFNGII SDIGGPTGNM YMMKCKKMEK
SGACNHKRCL YPEVCNGMNT DHKPLLNLLE KIRSIKGIKK VFVTSGIRAD LAVHDKINGK
KYIEAIAKNH VSGQLKMAPE SADEKVLKAM KKPDNKSFLE FCRIYKEYSR KFHKNQFISC
YFIAAHPEES EQKAFNTHNF IEKYLDFSPE QVQIFTPAPS TWSTCAYYTG LDEEGKDIYI
EKHLNRKEKY KEIIMGKGKI HKNYKRK
//
