ID V3ZQC6_LOTGI Unreviewed; 888 AA.
AC V3ZQC6;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=beta-mannosidase {ECO:0000256|ARBA:ARBA00012754};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase {ECO:0000256|ARBA:ARBA00033445};
GN ORFNames=LOTGIDRAFT_119184 {ECO:0000313|EMBL:ESO93598.1};
OS Lottia gigantea (Giant owl limpet).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX NCBI_TaxID=225164 {ECO:0000313|EMBL:ESO93598.1, ECO:0000313|Proteomes:UP000030746};
RN [1] {ECO:0000313|EMBL:ESO93598.1, ECO:0000313|Proteomes:UP000030746}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|ARBA:ARBA00004371}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
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DR EMBL; KB201891; ESO93598.1; -; Genomic_DNA.
DR RefSeq; XP_009055793.1; XM_009057545.1.
DR AlphaFoldDB; V3ZQC6; -.
DR STRING; 225164.V3ZQC6; -.
DR EnsemblMetazoa; LotgiT119184; LotgiP119184; LotgiG119184.
DR GeneID; 20231708; -.
DR KEGG; lgi:LOTGIDRAFT_119184; -.
DR CTD; 20231708; -.
DR HOGENOM; CLU_005015_3_1_1; -.
DR OMA; EFIYFSQ; -.
DR OrthoDB; 2504097at2759; -.
DR Proteomes; UP000030746; Unassembled WGS sequence.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000030746};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..888
FT /note="beta-mannosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004716489"
FT DOMAIN 81..148
FT /note="Glycosyl hydrolases family 2 sugar binding"
FT /evidence="ECO:0000259|Pfam:PF02837"
FT DOMAIN 806..886
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 888 AA; 101930 MW; 5118E9622BD001B3 CRC64;
MAANTVEITV LLLFSLINAS LGVVSVDLNG VWQISNENRG HQINGTVPGT VHLALYQNKM
IQDPYYRNND AKYRWIGLDD WTYTRKFQVS TSVFNSKRVI LVADGLDTFS TVYINGQSVG
VSDNMYMRYE YDIKNVIKNG TNTIEVKFRS AVKEAEKLAK ASSYNIPPDC KDPAQHGECH
VQFIRKAQCS FSWDWGPSFP VQGIWLVSSL DVELSYKNNK LHMSYCYYLD SNNTWSVDVE
VYFDVIKGSV TGSLETVLDT TPPIRLVTDI KLTNVENGAK YTINVPKDTP VDLWWPNGYG
KQSLYKLSVN FTGNTGLKES STRTIKIGFR TVEIVQDPVS LANKTYGLSF FYKVNDVPVF
FKGTNWIPSD SFLERVTDNK IYALLKSAAE VHINSMRVWG GGVYETDYFY QLADELGIMI
WSDFMFSVAL YPTNTAFLLS VTIETRQQIR RLKYHPCIAV WAGNNENELG LRQGWYGVKA
VDPYKSDYIK LYITTIGNVV KQEDKTRQYL SSSPSDGVYT KQQGGIAQNP ASEFYGDVHY
YNYINDLWNW ETYQIPRFAS EYGAQSWCNY ETVEKVFEAD DLDYWSDMAE WRQHHGLGNI
EMIVQAMLHF KLPNSSNRQQ RFDDLIYITQ IHQAMAMKVE TEHYRRLTNE LFDDGRGLTM
GALYWQLNDI WQAPTWASID YDLKWKMIHY YARHFFTPNL ISPFVNGSNL NIYMVNDEIP
NDVHRTQNDL QGLEFRAVND LKQLQKTSGL IFEKDLESVL NEGKCTDKKK CFLYFYLNSK
SHPVSVNWLP LSYFTGNDLT ASLGLHKAQI MITDVTAISA TEFNIILATN TVAPFVWINA
QGISGRFSDN GFLMKDPLMQ LKFTAWQPVK PDDFRDSLTV KSLMDIYH
//
