ID V4A8S7_LOTGI Unreviewed; 590 AA.
AC V4A8S7;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=LOTGIDRAFT_105466 {ECO:0000313|EMBL:ESO91445.1};
OS Lottia gigantea (Giant owl limpet).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX NCBI_TaxID=225164 {ECO:0000313|EMBL:ESO91445.1, ECO:0000313|Proteomes:UP000030746};
RN [1] {ECO:0000313|EMBL:ESO91445.1, ECO:0000313|Proteomes:UP000030746}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; KB202283; ESO91445.1; -; Genomic_DNA.
DR RefSeq; XP_009058134.1; XM_009059886.1.
DR AlphaFoldDB; V4A8S7; -.
DR STRING; 225164.V4A8S7; -.
DR EnsemblMetazoa; LotgiT105466; LotgiP105466; LotgiG105466.
DR GeneID; 20229985; -.
DR KEGG; lgi:LOTGIDRAFT_105466; -.
DR CTD; 20229985; -.
DR HOGENOM; CLU_008926_1_5_1; -.
DR OrthoDB; 2944799at2759; -.
DR Proteomes; UP000030746; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000030746};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 17..590
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5005148365"
FT DOMAIN 144..565
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 499
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 543
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 552
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 590 AA; 64892 MW; F5782F302810D462 CRC64;
MLILFLFGII LGQAASLKVS MKTHWSGGVT GSFCIPITQD LHGWKAHLIF DHVINKLDVS
TCIAEKKQGG KEFVLTNKPF NKEEHVGDKL CVDFVGHTDS DINPTATVSI EGMDGSGSSF
SHTTALYFTM EFFSPGGDRG LYDYSKPLAN SILFYNAQRS GKLPANNPIK WRGDSALKDG
SDVGVDLTGG WYDAGDHVKF GLPMAATAHL LLWSYIQWPD AYSSTSLTDK FFDMIKWPLD
YFLKCWIPSK NEFYMQVGNG DDDHHFWGRA EDMKMARPSL KNTASKPGSD VAGETAAAMA
AGSIAFKKRD HAYSTKLLTA AKSLYVFAKT YTGKYSQSVQ GAGQFYSSSG YTDELCEAAI
WLYRATKDQK YLNDAKGFVE TAWAWALSWD DKKVACQLML YQETQDDMAG KAVIGFMKSW
LPGGGITYTP CGLAWRDQWG STRYAANTAF LALMAADAGL EATKYRKWAQ SQINYILGQN
KQCFSYVIGF GKKFPRNPHH RSASCPMIPA PCSEANLHSS GPSPHILVGA LVGGPDAKGA
YTDKREDYVH NEVACDYNSG FHSALAGLIH LTHTKSLPRA DTPKCPCSVH
//
