ID V4KL17_EUTSA Unreviewed; 626 AA.
AC V4KL17;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=EUTSA_v10011318mg {ECO:0000313|EMBL:ESQ30617.1};
OS Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ30617.1, ECO:0000313|Proteomes:UP000030689};
RN [1] {ECO:0000313|EMBL:ESQ30617.1, ECO:0000313|Proteomes:UP000030689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA Mitchell-Olds T., Schumaker K.S., Wang X.;
RT "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL Front. Plant Sci. 4:46-46(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; KI517809; ESQ30617.1; -; Genomic_DNA.
DR RefSeq; XP_006393331.1; XM_006393269.1.
DR AlphaFoldDB; V4KL17; -.
DR STRING; 72664.V4KL17; -.
DR EnsemblPlants; ESQ30617; ESQ30617; EUTSA_v10011318mg.
DR GeneID; 18011479; -.
DR Gramene; ESQ30617; ESQ30617; EUTSA_v10011318mg.
DR KEGG; eus:EUTSA_v10011318mg; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR OMA; GFDKYYN; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000030689; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF186; ENDOGLUCANASE; 1.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000030689};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 27..626
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5005148402"
FT DOMAIN 534..614
FT /note="Carbohydrate binding"
FT /evidence="ECO:0000259|SMART:SM01063"
FT REGION 504..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 416
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 626 AA; 69190 MW; 9C56EE4826161F13 CRC64;
MKKFGGSLFG VSLLLTVLLA TATAAAEYYN YGNALDKTFL FFEAQRSGKL PATQRVKWRS
HSGLKDGLAQ GVSLEGGYYD AGDHVKFGLP MAFAVTMLSW AAVDNRKELS GSNQMQQTLS
SIKWGTDYFI KAHPQPNVLW GQVGDGESDH YCWMRAEDMT TSRTAYKLDQ YHPGSDLAGE
TAAALAAASL AFKPFNSSYS AILLSHAKEL FSFADKYRGL YTDSIPNAKA FYMSSGYSDE
LLWAAAWLHR ATGDQYYLKY AVDNAGYMGG TGWGMKEFSW DNKYAGVQIL LSKILLEGKG
GVYTSTLKQY QMKANYFTCA CLKKNGGYNI QTTPGGLMYV REWNNLQYAS AAAFLLAVYS
DYLSAANTKL NCPDGWVPPQ ALLDFARSQA DYILGKNSQG MSYLVGYGPK YPIRVHHRGA
SIASIFVQRS SVNCVQGFDS WYRRSQADPN VIHGALVGGP DHNDYYSDDR TNYEQSEPTL
SGTAPLVGLF SKLSGKLGSY GGGYSKPYQT TKPPASSYKA TPTTYSPKQS GAPIEFLHSI
TANWMAWNTR YYRHKVIIKN NSQKPISDLK LKIEDLSGPI WGLNPTGQKN TYQLPQWQKT
LRAGQAYDFV YVQGGPQAKV SVLSYN
//
