ID   V4KMM9_EUTSA            Unreviewed;       778 AA.
AC   V4KMM9;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   27-MAR-2024, entry version 46.
DE   RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE            EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN   ORFNames=EUTSA_v10018154mg {ECO:0000313|EMBL:ESQ28548.1};
OS   Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX   NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ28548.1, ECO:0000313|Proteomes:UP000030689};
RN   [1] {ECO:0000313|EMBL:ESQ28548.1, ECO:0000313|Proteomes:UP000030689}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA   Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA   Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA   Mitchell-Olds T., Schumaker K.S., Wang X.;
RT   "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL   Front. Plant Sci. 4:46-46(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906}.
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DR   EMBL; KI517953; ESQ28547.1; -; Genomic_DNA.
DR   EMBL; KI517953; ESQ28548.1; -; Genomic_DNA.
DR   RefSeq; XP_006391261.1; XM_006391199.1.
DR   RefSeq; XP_006391262.1; XM_006391200.1.
DR   AlphaFoldDB; V4KMM9; -.
DR   STRING; 72664.V4KMM9; -.
DR   EnsemblPlants; ESQ28547; ESQ28547; EUTSA_v10018154mg.
DR   EnsemblPlants; ESQ28548; ESQ28548; EUTSA_v10018154mg.
DR   GeneID; 18008390; -.
DR   Gramene; ESQ28547; ESQ28547; EUTSA_v10018154mg.
DR   Gramene; ESQ28548; ESQ28548; EUTSA_v10018154mg.
DR   KEGG; eus:EUTSA_v10018154mg; -.
DR   eggNOG; KOG0167; Eukaryota.
DR   OMA; YDFDMNE; -.
DR   OrthoDB; 2721872at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000030689; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR23315; U BOX DOMAIN-CONTAINING; 1.
DR   PANTHER; PTHR23315:SF284; U-BOX DOMAIN-CONTAINING PROTEIN 7; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00185; ARM; 5.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000030689};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT   DOMAIN          271..345
FT                   /note="U-box"
FT                   /evidence="ECO:0000259|PROSITE:PS51698"
FT   REPEAT          463..509
FT                   /note="ARM"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
FT   REGION          225..245
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          705..759
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        225..241
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        705..724
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   778 AA;  85925 MW;  F58E80B5CD1D00F8 CRC64;
     MDVTELEENL FAASDAKLHG DMCKELSGVL CKVLSIFPSL EGARPRSKSG IQALCSLHIA
     LEKAKNILQH CSECSKLYLA ITGDAVLLKF EKARTALIDS LKRVEDIVPS SIGSQILEIV
     GELENTRFLL DPSEKEVGDQ IIALLQQGKK FDNCNDNTEL EIFHRAATRL SITSSRVALA
     ERRALKKLID RARTEEDKRK ESIVAYLLHL MRKCSKLFRS EVLDENDSQG STPCSPTDQG
     FNEDRRSVHG FGRQLSRFGS MNSPKSGQMP VPPEELRCPI SLQLMCDPVI IASGQTYERV
     CIEKWFSDGH NTCPKTQQEL PHLSLTPNNC VKGLIASWCE QNGTQIPSEP PESLDLDYWR
     LALSDSDSTR SKSVNSIGSC KSKSIKIIPL EENGTTVANT EESFVSDVDD EKDSHVNVLK
     RYQDLLAILN EEDDLEKKVK VVEKIRLLLK DDEEARIVMG ANGFVQALLR FLGSAVDENN
     VAAQETGAMA LFNLAVNNNR NKELMLTSGV IPLLEKMISS SESQGSATAL YLNLSCLEEA
     KSVIGSSQAV PFLVQILQGE TDNQCKLDAL HALYNLSTYS PNISALLSSN IIKSLQGLLA
     STGEHLWVEK SLAVLLNLAS SQEGKDEAVS SQGMISSLAT VLDMGDTLEQ EQAVSCLLIL
     CNGRESCIQM VLQEGVIPSL VSISVNGTPR GREKSQKLLM LFREQRQQRD QPSPNRDEPP
     QKEAPKKSLS APMFVHGSAS ASAPEYEPRV LSKSMSRRKS LARPFSFFWK KSYSVHHQ
//