ID V4LMA7_EUTSA Unreviewed; 614 AA.
AC V4LMA7;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Purple acid phosphatase {ECO:0000256|RuleBase:RU361203};
DE EC=3.1.3.2 {ECO:0000256|RuleBase:RU361203};
GN ORFNames=EUTSA_v10012988mg {ECO:0000313|EMBL:ESQ43562.1};
OS Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ43562.1, ECO:0000313|Proteomes:UP000030689};
RN [1] {ECO:0000313|EMBL:ESQ43562.1, ECO:0000313|Proteomes:UP000030689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA Mitchell-Olds T., Schumaker K.S., Wang X.;
RT "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL Front. Plant Sci. 4:46-46(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC Evidence={ECO:0000256|RuleBase:RU361203};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple
CC acid phosphatase family. {ECO:0000256|ARBA:ARBA00008723,
CC ECO:0000256|RuleBase:RU361203}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KI517464; ESQ43562.1; -; Genomic_DNA.
DR RefSeq; XP_006402109.1; XM_006402046.1.
DR AlphaFoldDB; V4LMA7; -.
DR STRING; 72664.V4LMA7; -.
DR EnsemblPlants; ESQ43562; ESQ43562; EUTSA_v10012988mg.
DR GeneID; 18017640; -.
DR Gramene; ESQ43562; ESQ43562; EUTSA_v10012988mg.
DR KEGG; eus:EUTSA_v10012988mg; -.
DR eggNOG; KOG1378; Eukaryota.
DR OMA; NEMTLTW; -.
DR OrthoDB; 456532at2759; -.
DR Proteomes; UP000030689; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0003993; F:acid phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd00839; MPP_PAPs; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR Gene3D; 2.60.40.380; Purple acid phosphatase-like, N-terminal; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR040974; Fn3_PAP.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041792; MPP_PAP.
DR InterPro; IPR008963; Purple_acid_Pase-like_N.
DR InterPro; IPR015914; Purple_acid_Pase_N.
DR InterPro; IPR025733; Purple_acid_PPase_C_dom.
DR PANTHER; PTHR45778:SF6; INACTIVE PURPLE ACID PHOSPHATASE 24-RELATED; 1.
DR PANTHER; PTHR45778; PURPLE ACID PHOSPHATASE-RELATED; 1.
DR Pfam; PF17808; fn3_PAP; 1.
DR Pfam; PF00149; Metallophos; 1.
DR Pfam; PF14008; Metallophos_C; 1.
DR Pfam; PF16656; Pur_ac_phosph_N; 1.
DR SUPFAM; SSF56300; Metallo-dependent phosphatases; 1.
DR SUPFAM; SSF49363; Purple acid phosphatase, N-terminal domain; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|RuleBase:RU361203};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Reference proteome {ECO:0000313|Proteomes:UP000030689};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU361203};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT CHAIN 21..614
FT /note="Purple acid phosphatase"
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT /id="PRO_5005148453"
FT DOMAIN 50..167
FT /note="Purple acid phosphatase Fn3-like"
FT /evidence="ECO:0000259|Pfam:PF17808"
FT DOMAIN 172..278
FT /note="Purple acid phosphatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16656"
FT DOMAIN 290..505
FT /note="Calcineurin-like phosphoesterase"
FT /evidence="ECO:0000259|Pfam:PF00149"
FT DOMAIN 530..589
FT /note="Iron/zinc purple acid phosphatase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14008"
SQ SEQUENCE 614 AA; 69045 MW; D6EDC50880C0B2AA CRC64;
MVRDLFSLLC LIVHVASSLC SSHENGRGDQ ALSQIDIYST SFALHHSATI HASPHVLGFK
GEDTEWVNVD ISNPEPSSDD WVGVFSPAKF DSSSCAPTDG REIAPFICSA PIKYMYAKSN
PDYMKTGKAV LKFLLINQRA DFSFALFTGG LSNPNLVAVS NHVSFTNPKA PVYPRLALGK
NWDHMSVTWT SGYNIGEAIP FVEWSGKGIS SRRSPAGTLT FTRNSMCGAP ARTVGWRDPG
FIHTAFLKDL WPNLKYTYRM GHELVNGSII WSKNYTFKSS PYPGQDSLQR VIIFGDMGKG
ERDGSNEYND YQPGSLNTTD QVIKDLKNID IVFHIGDITY ANGYISQWDQ FTAQVEPIAS
TVPYMIASGN HERDWPNSGS FYGGKDSGGE CGVPAETMFD YPAENKAKFW YSADYGMFRF
CVADTEHDWR EGSEQYRFIE RCLASVDRKT QPWLIFIAHR VLGYSTNDWY GQEGSFEEPM
GRESLQKLWQ KYKVDIAFYG HVHNYERTCP IYQNQCMDNE KSYYSGAFKG TIHVVVGGAG
SHLSSFSSLK PKWSIFRDYD YGFVKLTAFD HSSLLFEYKK SSNGEVYDSF TVSREYRDVL
ACVRDSCDPT TLAS
//
