ID V4LYE9_EUTSA Unreviewed; 547 AA.
AC V4LYE9;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Beta-hexosaminidase {ECO:0000256|PIRNR:PIRNR001093};
DE EC=3.2.1.52 {ECO:0000256|PIRNR:PIRNR001093};
GN ORFNames=EUTSA_v10010264mg {ECO:0000313|EMBL:ESQ44933.1};
OS Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ44933.1, ECO:0000313|Proteomes:UP000030689};
RN [1] {ECO:0000313|EMBL:ESQ44933.1, ECO:0000313|Proteomes:UP000030689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA Mitchell-Olds T., Schumaker K.S., Wang X.;
RT "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL Front. Plant Sci. 4:46-46(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine
CC residues in N-acetyl-beta-D-hexosaminides.; EC=3.2.1.52;
CC Evidence={ECO:0000256|ARBA:ARBA00001231,
CC ECO:0000256|PIRNR:PIRNR001093};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 20 family.
CC {ECO:0000256|ARBA:ARBA00006285, ECO:0000256|PIRNR:PIRNR001093}.
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DR EMBL; KI517435; ESQ44933.1; -; Genomic_DNA.
DR RefSeq; XP_006403480.1; XM_006403417.1.
DR AlphaFoldDB; V4LYE9; -.
DR STRING; 72664.V4LYE9; -.
DR EnsemblPlants; ESQ44933; ESQ44933; EUTSA_v10010264mg.
DR GeneID; 18020780; -.
DR Gramene; ESQ44933; ESQ44933; EUTSA_v10010264mg.
DR KEGG; eus:EUTSA_v10010264mg; -.
DR eggNOG; KOG2499; Eukaryota.
DR OMA; NYCVEPP; -.
DR OrthoDB; 178991at2759; -.
DR Proteomes; UP000030689; Unassembled WGS sequence.
DR GO; GO:0005773; C:vacuole; IEA:EnsemblPlants.
DR GO; GO:0004563; F:beta-N-acetylhexosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0102148; F:N-acetyl-beta-D-galactosaminidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd06562; GH20_HexA_HexB-like; 1.
DR Gene3D; 3.30.379.10; Chitobiase/beta-hexosaminidase domain 2-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR025705; Beta_hexosaminidase_sua/sub.
DR InterPro; IPR015883; Glyco_hydro_20_cat.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR029018; Hex-like_dom2.
DR InterPro; IPR029019; HEX_eukaryotic_N.
DR PANTHER; PTHR22600; BETA-HEXOSAMINIDASE; 1.
DR PANTHER; PTHR22600:SF40; BETA-HEXOSAMINIDASE 1; 1.
DR Pfam; PF00728; Glyco_hydro_20; 1.
DR Pfam; PF14845; Glycohydro_20b2; 1.
DR PIRSF; PIRSF001093; B-hxosamndse_ab_euk; 1.
DR PRINTS; PR00738; GLHYDRLASE20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF55545; beta-N-acetylhexosaminidase-like domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR001093};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR001093};
KW Reference proteome {ECO:0000313|Proteomes:UP000030689};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..547
FT /note="Beta-hexosaminidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004724196"
FT DOMAIN 38..166
FT /note="Beta-hexosaminidase eukaryotic type N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14845"
FT DOMAIN 191..495
FT /note="Glycoside hydrolase family 20 catalytic"
FT /evidence="ECO:0000259|Pfam:PF00728"
FT ACT_SITE 338
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001093-1"
SQ SEQUENCE 547 AA; 62163 MW; D9A2E8E586B1FBEC CRC64;
MAPNLLHALL IFLSLSIITS SLSSPPPVTV HQDYPPYLWP LPAEFSSGDE TLSVDPSLSL
IVAGNGGGSP IVKAAFERYM GMIFKHSFGR ASSLLSRIRF LRMVEYDITS LKIVVHSDSE
ELQFGVDESY TLMVSKKKEQ SIVGAATIEA NTVYGALRGL ETFSQLCAFD YLTKSVQIYN
APWFIQDKPR FQYRGLLIDT SRHYLPIDVI KQIIESMSFA KLNVLHWHIV DEQSFPLETP
TYPNLWKGAY SRWERYTVED ASEIVRFAKM RGINVMAEVD VPGHAESWGT GYPDLWPSLS
CREPLDVTKN FTFDVISGIL SDMRKIFPFE FFHLGGDEVN TDCWKNTTHV KEWLQDRNFT
TKDAYKYFVL RAQQIAISKN WTPVNWEETF SSFGKDLDPK TVVQNWLVSD ICQKAVAKGF
RCIFSNQGYW YLDHLDVPWD EVYNTEPLNG IEDPSLQKLV IGGEVCMWGE TADTSVVLQT
IWPRAAAAAE RMWSTREAVS KGNITLNALP RLHYFRCLLN NRGVPAAPVD NWYARRPPYG
PGSCYAQ
//
