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Database: UniProt
Entry: V4M593_EUTSA
LinkDB: V4M593_EUTSA
Original site: V4M593_EUTSA 
ID   V4M593_EUTSA            Unreviewed;       950 AA.
AC   V4M593;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   25-APR-2018, entry version 18.
DE   RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE            Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE            EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN   ORFNames=EUTSA_v10020003mg {ECO:0000313|EMBL:ESQ47463.1};
OS   Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Eutremeae;
OC   Eutrema.
OX   NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ47463.1, ECO:0000313|Proteomes:UP000030689};
RN   [1] {ECO:0000313|EMBL:ESQ47463.1, ECO:0000313|Proteomes:UP000030689}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA   Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J.,
RA   Jenkins J., Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA   Mitchell-Olds T., Schumaker K.S., Wang X.;
RT   "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL   Front. Plant Sci. 4:46-46(2013).
CC   -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC       conversion of glucosylceramide to free glucose and ceramide.
CC       {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- CATALYTIC ACTIVITY: D-glucosyl-N-acylsphingosine + H(2)O = D-
CC       glucose + N-acylsphingosine. {ECO:0000256|PIRNR:PIRNR028944}.
CC   -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase
CC       family. {ECO:0000256|PIRNR:PIRNR028944}.
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DR   EMBL; KI517408; ESQ47463.1; -; Genomic_DNA.
DR   EMBL; KI517408; ESQ47464.1; -; Genomic_DNA.
DR   RefSeq; XP_006406010.1; XM_006405947.1.
DR   RefSeq; XP_006406011.1; XM_006405948.1.
DR   KEGG; eus:EUTSA_v10020003mg; -.
DR   KO; K17108; -.
DR   Proteomes; UP000030689; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR   InterPro; IPR006775; GH116_catalytic.
DR   InterPro; IPR024462; GH116_N.
DR   Pfam; PF04685; DUF608; 1.
DR   Pfam; PF12215; Glyco_hydr_116N; 1.
DR   PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000030689};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW   Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW   Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW   Reference proteome {ECO:0000313|Proteomes:UP000030689}.
FT   DOMAIN      102    421       Glyco_hydr_116N. {ECO:0000259|Pfam:
FT                                PF12215}.
FT   DOMAIN      535    896       DUF608. {ECO:0000259|Pfam:PF04685}.
SQ   SEQUENCE   950 AA;  106241 MW;  DFEE2A7A70665BC4 CRC64;
     MVGATLFHRR KHSWPAEEFI SRNTLQLLDF DSAAPPPHAW RRRLNCHANI LKEFTITFRE
     AIKMVRLGIR LWSYVREEAS HGRKAPIDPF TKENCKPSAS QGVPLGGMGS GSISRGFRGE
     FKQWQITPGT CDPSPMMSNQ FSIFISRDGG HKKYASVLAP GQHGSLGKSR DKGISSWGWN
     LNGQHSTYHA LFPRAWTIYD GEPDPELKIS CRQISPFIPN NYRDSSLPAS VFVYTLVNTG
     KERAKVSLLF TWANSIGGTS HMSGGHVNEP FIGEDGVSGV LLHHKTGKGN PPVTFAISAC
     ETQNVNVTVL PCFGLSEDSS MTAKDMWDMM EKDGKFDQAN FNSGPSMPSS AGDTICAAVS
     ASAWVEAHGR CTVSFALSWS SPKVKFSKGS TYDRRYTKYY GTSPRAALDL VHDALTHYKR
     WEGDIEAWQN PILGDDRLPE WYKFTLFNEL YFLVAGGTVW IDSASLHANG DSQLQQSNSR
     NPDGKASGVD SKDQQNNLND CDSNGIKSNG EASVIHQKNG LFVDTRHVDD DDDVGRFLYL
     EGVEYVMWNT YDVHFYASYA LLMLFPKIEL NIQRDFAKAV LSEDGRKVKF LAEGNWGIRK
     VRGAVPHDLG MHDPWNEMNA YNIHDTSKWK DLNPKFVLQV YRDFAATGDY QFGIDVWPAV
     RAAMEYMEQF DRDNDDLIEN DGFPDQTYDT WTVHGVSAYC GCLWLAALQA AAAMALQIGD
     KFFAELCKNK FLNAKAALET KLWNGSYLNY DSGASSNSKS IQTDQLAGQW YTASSGLPPI
     FEESKIRSSL QKIFDFNVMK TKGGRMGAVN GMHPDGKVDE TCMQSREIWT GVTYAAAATM
     ILSGMEEQGF TTAEGIFTAG WSEEGFGYWF QTPEGWTMDG HYRSLIYMRP LAIWGMQWAL
     SLPKAILDAP KINMMDRVHM SPRSRRFSHN VKVVKHKAKC FGNSKLNCTC
//
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