ID V4MV53_EUTSA Unreviewed; 1194 AA.
AC V4MV53;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=EUTSA_v10006571mg {ECO:0000313|EMBL:ESQ35991.1};
OS Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ35991.1, ECO:0000313|Proteomes:UP000030689};
RN [1] {ECO:0000313|EMBL:ESQ35991.1, ECO:0000313|Proteomes:UP000030689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA Mitchell-Olds T., Schumaker K.S., Wang X.;
RT "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL Front. Plant Sci. 4:46-46(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA2/NAM7 helicase family.
CC {ECO:0000256|ARBA:ARBA00007913}.
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DR EMBL; KI517683; ESQ35991.1; -; Genomic_DNA.
DR RefSeq; XP_006417638.1; XM_006417575.1.
DR AlphaFoldDB; V4MV53; -.
DR EnsemblPlants; ESQ35991; ESQ35991; EUTSA_v10006571mg.
DR Gramene; ESQ35991; ESQ35991; EUTSA_v10006571mg.
DR KEGG; eus:EUTSA_v10006571mg; -.
DR Proteomes; UP000030689; Unassembled WGS sequence.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0017116; F:single-stranded DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:UniProtKB-UniRule.
DR CDD; cd18041; DEXXQc_DNA2; 1.
DR CDD; cd22318; DNA2_N-like; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR022765; Dna2/Cas4_DUF83.
DR InterPro; IPR026851; Dna2/JHS1_DEXXQ-box.
DR InterPro; IPR041679; DNA2/NAM7-like_C.
DR InterPro; IPR041677; DNA2/NAM7_AAA_11.
DR InterPro; IPR014808; DNA_replication_fac_Dna2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR PANTHER; PTHR36531; CRISPR-ASSOCIATED EXONUCLEASE CAS4; 1.
DR PANTHER; PTHR36531:SF6; DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE; 1.
DR Pfam; PF13086; AAA_11; 2.
DR Pfam; PF13087; AAA_12; 1.
DR Pfam; PF01930; Cas_Cas4; 1.
DR Pfam; PF08696; Dna2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000030689}.
FT DOMAIN 360..560
FT /note="DNA replication factor Dna2 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08696"
FT DOMAIN 568..677
FT /note="DUF83"
FT /evidence="ECO:0000259|Pfam:PF01930"
FT DOMAIN 926..1013
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1026..1093
FT /note="DNA2/NAM7 helicase helicase"
FT /evidence="ECO:0000259|Pfam:PF13086"
FT DOMAIN 1102..1183
FT /note="DNA2/NAM7 helicase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13087"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..73
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1194 AA; 132315 MW; C0747FFE48D96DBC CRC64;
MPPRKKAKSS ALKSNKQSSA NQSSQPSSFG IQQLFLRHIQ NSQSTSSTHV STAEPVDPEN
VNGLATNTGV LRPHNPPITL DEKPDESKDV DQQPTEASPE VSKNIKRFKF SPGMLIKPSQ
DDGGEEITWK ISPVNERLRA AAKQIPKMMD LTEYSVGVKS STIRPCSQQI VQKQCPTPVI
TSKVEQWLSS PSKKAFKRPA FAATRVVERV NPSPAADFEI VNTSSSGDSP FQTPPSLSCS
HNKLPCTVTC SAACGFMGAG QHKKALLELL DQVEDVISVD DKTADDVRTV TPQVRVKDDN
ICSVVDSAVD GGPITLLKKH TSVNPDNHFL VLEVSEKRGS VGSSKVQCPY KVLRLLDEYT
GKVCALYLWD EWFYSTVSPG DSINVIGDFD GDGKCDVDHQ NNFLIVHPDT LIAGTKVAAS
FGCPRRTVLD ERLRSNEHAT AALLGTLLHQ VFQAGLTQES PSVDSLQEYA SIVIKKNIES
LYACGVHEGD VKATILGAIP KMLNWIHNFR YSKDSRISNV DFGSTNGQKM IKISEVIDIE
EMSWAPKYGL KGMIDASVRV IVESEMNTAN EKIMPLEFKS GKAPSGQSSM EHSAQVILYT
LLMSERYLKH IDNGLLYYLQ SDQTQGISVQ RSDLVGLIIR RNELANDILV ASTTQQLPPM
LRNPNMCRNC RHLDVCTIYH KADGGNTESS GLGDLFDTHV SHLSTLHFDF LRHWDRLIDL
ESREMQLLRN DIAHPHGSKG SHSASYLSSM VLDMKDGFQH HNAHKETRFI YRFVRQNSSE
TGERVPSEDT IRTGTPATDD LDCRLRTGDR VILRTEVSHL TVANGTIADI SRTHVSVSLS
KRLRLPWSEP SSEVSNLSHE LWRIYKDEFM TSFSIMRFNL MQLFIQNERS IGIRKMIVDL
EPPRFENGSI LSQDPAISYI WSEKSLNNDQ RQAILKILTA KDYALILGMP GTGKTSTMVH
AVKALLIRGS SVLLASYTNS AVDNLLIKLK AQGIEFLRIG RDEAVHEEVR ESCFSAMDMC
SVDDIKTKLD QVKVVASTCL GINSPFLVNR RFDVCIIDEA GQIALPVSIG PLLFASTFVL
VGDHYQLPPL VQSTEARENG MGISLFRRLS EAHPQAISVL QNQYRMCRGI MELSNALIYG
DRLCCGSAEV ANATLVLSTF SSTSSWLRKV LEPTRTVVFI NTGFSLLTSC LFFT
//
