ID V4N5E4_9CAUL Unreviewed; 1130 AA.
AC V4N5E4;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 44.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=AEYBE204_18445 {ECO:0000313|EMBL:ESQ77062.1};
OS Asticcacaulis sp. YBE204.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Asticcacaulis.
OX NCBI_TaxID=1282363 {ECO:0000313|EMBL:ESQ77062.1, ECO:0000313|Proteomes:UP000017808};
RN [1] {ECO:0000313|EMBL:ESQ77062.1, ECO:0000313|Proteomes:UP000017808}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YBE204 {ECO:0000313|EMBL:ESQ77062.1,
RC ECO:0000313|Proteomes:UP000017808};
RX PubMed=24463524; DOI=10.1038/nature12900;
RA Jiang C., Brown P.J., Ducret A., Brun Y.V.;
RT "Sequential evolution of bacterial morphology by co-option of a
RT developmental regulator.";
RL Nature 506:489-493(2014).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESQ77062.1}.
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DR EMBL; AWGF01000020; ESQ77062.1; -; Genomic_DNA.
DR AlphaFoldDB; V4N5E4; -.
DR STRING; 1282363.AEYBE204_18445; -.
DR PATRIC; fig|1282363.3.peg.3582; -.
DR Proteomes; UP000017808; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000017808}.
FT DOMAIN 598..759
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 780..934
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1130 AA; 125296 MW; F013A177B5AEBA5F CRC64;
MFVARDYARM NGVAEELGFY APDLGIIRFP AWDCLPYDRV SPTAGLIAQR MHGLTRLQAL
SGAKPAKPVL ILTTASALSQ KVPPREAMKD SFLSLRPGQS VDTNELEAYF TRNGYNRVST
VSERGEFAVR GGIVDVFSPS QSEPVRLDFF GDTLETIRSF DPETQRSLKQ VTQIDFMAVS
EILLDEKSIS RFRQGYLENF GAPGDDPLYA AVSSGIRRQG VETLLPLFYN HLDSVFDYLP
EGTLILLDAL ADAAFDERQA TIIDAYESRK GSIDARVMAK GAVKLDSGYR ALPPERLYLK
DNQWREGLTR GRVRQFESFQ RDDAGHVDLG ARTGRSFAPE RLQDSVNLFS AVAEHARAKS
KMGKRVLFAS WTEGSSDRLG AMLADHGLDK TRLAKDYADA VAFGVAGTKA KPIQRAVLPL
DQGYETEGLV VISETDILGD RLARPRKRRR ANNFLAEASS LSMGDLVVHI EHGIGRYEGL
KTLSVNDAPH DCLELQYAAD AKLYLPVENI DLLTRYGADS DSAQLDKLGS ASWQARKSKA
KEKLREMADG LIQLAAARAL KVGQQIDPPS GLYDEFCAQF PYEETEDQLN AIHDVLEDLA
KGQPMDRLIC GDVGFGKTEV ALRAAFVVAM SGQQVAIICP TTLLARQHFK TISQRFQGWP
IRVRHLSRMV TKKEADETRD GLKNGEVEVV IGTHALLAEQ VSFKDLGLVI VDEEQHFGVK
HKEKLKSFRA DVHMLTLSAT PIPRTLQMAL SGIREMSIIA TPPVDRLAVR TYVLPFDGVS
IREALLREKY RGGQAYYVVP RLKDLPDVER FLREQVPEVR FIVGHGQMTP TQLEEVMTAF
YDGQYDVLLA TTIVESGLDV PTANTLIVHR ADMFGLSQLY QIRGRVGRSK TRAFAYLTTQ
PHHILSDASE KRLKVLQSLD NLGAGFQLAS HDLDIRGGGN LLGNEQSGHI REIGVELYQQ
MLEDAVAELR SRNDQVVDDR SWSPQINAGA AIMIPEDYIP DLNTRLSLYR RVSEAEKRED
REALAAELID RFGPIPEAAE QLLKVVGIKG LCREANVAKI DVGPKGAVIT FRNDTFANPM
GLIGLIQARP NDWKLRPDQK LLVRGEWPEA AARLVAAEKI MTQLAGVATG
//
