ID V4P2S7_EUTSA Unreviewed; 506 AA.
AC V4P2S7;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=EUTSA_v10027533mg {ECO:0000313|EMBL:ESQ53671.1};
OS Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ53671.1, ECO:0000313|Proteomes:UP000030689};
RN [1] {ECO:0000313|EMBL:ESQ53671.1, ECO:0000313|Proteomes:UP000030689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA Mitchell-Olds T., Schumaker K.S., Wang X.;
RT "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL Front. Plant Sci. 4:46-46(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KI517384; ESQ53671.1; -; Genomic_DNA.
DR RefSeq; XP_006412218.1; XM_006412155.1.
DR AlphaFoldDB; V4P2S7; -.
DR STRING; 72664.V4P2S7; -.
DR EnsemblPlants; ESQ53671; ESQ53671; EUTSA_v10027533mg.
DR GeneID; 18029101; -.
DR Gramene; ESQ53671; ESQ53671; EUTSA_v10027533mg.
DR KEGG; eus:EUTSA_v10027533mg; -.
DR eggNOG; ENOG502QPKZ; Eukaryota.
DR OMA; NPREIGI; -.
DR OrthoDB; 641887at2759; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000030689; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF88; 3-KETOACYL-COA SYNTHASE 18; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000030689};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 12..31
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 71..368
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 385..465
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 223
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 302
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 387
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 391
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 420
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 424
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 506 AA; 56557 MW; A1C4CF3C239A9086 CRC64;
MTSVNVKLIY HYVITNFFNL CFFPLTAIVA GKAYRLTIND LHIFYHSYLQ HNLITITLLF
ALTVFGSVLY ILTRPKPVYL VDHSCYLPPP HHRASISKVM DIFYQVRKAD PLRNVSSDDS
SWLDFLRKVQ ERSGLGDETH GPEGLLEVPP RKTFAAAREE TEQVIIGALE NLFENTKVNP
KEIGILVVNS SMFNPTPSLS AMVVNTFKLR SNIRSFNLGG MGCSAGVIAI DMAKDLLHVH
KNTYALVVST ENITYNIYAG DNRSMMVSNC LFRVGGAAIL LSNKPGDRRR SKYKLAHTVR
THTGADDKSF RCVQQEDDES GKTGVCLSKD ITGVAGRTVQ KNIAALGPLV LPLSEKFLFF
VTFVAKKLLK DKIKHYYVPD FKLAIDHFCI HAGGRAVIDV LEKSLGLSPI DVEASRSTLH
RFGNTSSSSI WYELAYIEAK GRMKKRNKVW QIALGSGFKC NSAVWVALRN VKASTNSPWE
HCIDRYPVQI DSDSAKSETR VQNGRS
//
