ID V5ASX3_TRYCR Unreviewed; 568 AA.
AC V5ASX3;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=tRNA (Uracil-5-)-methyltransferase {ECO:0008006|Google:ProtNLM};
GN ORFNames=TCDM_08135 {ECO:0000313|EMBL:ESS63910.1};
OS Trypanosoma cruzi Dm28c.
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.
OX NCBI_TaxID=1416333 {ECO:0000313|EMBL:ESS63910.1, ECO:0000313|Proteomes:UP000017861};
RN [1] {ECO:0000313|EMBL:ESS63910.1, ECO:0000313|Proteomes:UP000017861}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Dm28c {ECO:0000313|EMBL:ESS63910.1,
RC ECO:0000313|Proteomes:UP000017861};
RX PubMed=24482508;
RA Grisard E.C., Teixeira S.M., de Almeida L.G., Stoco P.H., Gerber A.L.,
RA Talavera-Lopez C., Lima O.C., Andersson B., de Vasconcelos A.T.;
RT "Trypanosoma cruzi Clone Dm28c Draft Genome Sequence.";
RL Genome Announc. 2:e01114-13(2014).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESS63910.1}.
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DR EMBL; AYLP01000107; ESS63910.1; -; Genomic_DNA.
DR AlphaFoldDB; V5ASX3; -.
DR EnsemblProtists; ESS63910; ESS63910; TCDM_08135.
DR VEuPathDB; TriTrypDB:TCDM_08135; -.
DR Proteomes; UP000017861; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR045850; TRM2_met.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR PANTHER; PTHR45904; TRNA (URACIL-5-)-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR45904:SF2; TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG A; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000017861};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 509
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 379
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 429
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 481
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 568 AA; 62129 MW; 378FA3B06ECFA3A9 CRC64;
MGDESSGSKE ESSGVPDDRV ILHEHSNENL LKFDTHDKYS TISAIKKMIM KSLPPPKTNA
PPQPLFNGLL RMGKNPNTPL LFMAFRTPED STAAAELLRG VFFRGRKQWQ ELPVTQRDLQ
LTHKGSSRKR SRGGEVLGQS KVAQWEGLAM EEQLARKKAH CLRVTEAIAP PGDKYAELFA
GVHVSPELTG YRNHVQLSFG YSETGEPSIG FLKGAMIEGT CAIHSALGKD IVTMNPLAKQ
VAEAVMSVCH LFLLPEKGGL MVFDKVKQQG FWRKLQVRHN VLGEVMVDME LDIDSAEASV
VSEVKTHLIN TLFSEGLRAK LSMISGKETA SVVSAQYHHG TGISSSSVDE PRHILFGTPT
LTEHLLGLRF ELSPTSFFQV NTSGMELLLC ETAKVAALGP KTTLLDLCCG TGVIGLTLAK
HVKRVIGIEL VESAVSDARQ NAKRNDIANA TFCSGRVEHL LPDIINSLPE EDKTDIVAIL
DPPRAGVTPT VLKWIRGTPT IRRVVYISCE QKALERDCPS LTKSTTKAYR GLPFELVASF
AVDLFPHTPH VEMVAVLARL QEESVSGG
//
