ID V5BK37_9GAMM Unreviewed; 790 AA.
AC V5BK37;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Putative tyrosine-protein kinase EpsB {ECO:0000313|EMBL:ESS73670.1};
DE EC=2.7.10.- {ECO:0000313|EMBL:ESS73670.1};
GN Name=epsB {ECO:0000313|EMBL:ESS73670.1};
GN ORFNames=MGMO_13c00040 {ECO:0000313|EMBL:ESS73670.1};
OS Methyloglobulus morosus KoM1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methyloglobulus.
OX NCBI_TaxID=1116472 {ECO:0000313|EMBL:ESS73670.1, ECO:0000313|Proteomes:UP000017842};
RN [1] {ECO:0000313|EMBL:ESS73670.1, ECO:0000313|Proteomes:UP000017842}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KoM1 {ECO:0000313|EMBL:ESS73670.1,
RC ECO:0000313|Proteomes:UP000017842};
RX PubMed=24356841;
RA Poehlein A., Deutzmann J.S., Daniel R., Simeonova D.D.;
RT "Draft Genome Sequence of the Methanotrophic Gammaproteobacterium
RT Methyloglobulus morosus DSM 22980 Strain KoM1.";
RL Genome Announc. 1:e01078-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000256|ARBA:ARBA00001074};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the etk/wzc family.
CC {ECO:0000256|ARBA:ARBA00008883}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESS73670.1}.
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DR EMBL; AYLO01000013; ESS73670.1; -; Genomic_DNA.
DR AlphaFoldDB; V5BK37; -.
DR STRING; 1116472.MGMO_13c00040; -.
DR PATRIC; fig|1116472.3.peg.469; -.
DR eggNOG; COG0489; Bacteria.
DR eggNOG; COG3206; Bacteria.
DR OrthoDB; 9775724at2; -.
DR Proteomes; UP000017842; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05387; BY-kinase; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR025669; AAA_dom.
DR InterPro; IPR032807; GNVR.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005702; Wzc-like_C.
DR NCBIfam; TIGR01007; eps_fam; 1.
DR PANTHER; PTHR32309; TYROSINE-PROTEIN KINASE; 1.
DR PANTHER; PTHR32309:SF13; TYROSINE-PROTEIN KINASE ETK-RELATED; 1.
DR Pfam; PF13614; AAA_31; 1.
DR Pfam; PF13807; GNVR; 1.
DR Pfam; PF02706; Wzz; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF57997; Tropomyosin; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:ESS73670.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000017842};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ESS73670.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Tyrosine-protein kinase {ECO:0000256|ARBA:ARBA00023137}.
FT TRANSMEM 39..57
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 23..117
FT /note="Polysaccharide chain length determinant N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02706"
FT DOMAIN 400..479
FT /note="Tyrosine kinase G-rich"
FT /evidence="ECO:0000259|Pfam:PF13807"
FT DOMAIN 557..712
FT /note="AAA"
FT /evidence="ECO:0000259|Pfam:PF13614"
FT COILED 301..393
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 790 AA; 87494 MW; 445A49F326EE72B8 CRC64;
MYLKDEMTAL PPPFGGFQVQ EPSISIRDYV DLLFEGRKII LSVLLSVLLL TAVYLVLAPR
TYKADALLRI DRNKALLAAT IRSETNASST EAESPRAQRE VEILRSRSVL GKVVDDLNLM
VEYSPSYFPI IGEAIARRHD VHDGVAEAWL GFSRWAWGGE KLNIATFNVP DRYLDKEFTL
VALENGRFRL LGPKGEDLVE GQVGETVTAD MGEAEPVAIK VSELAGHPGT HFELTRKTSL
TAIETLQKAF FVKEISKDTD ILSVELKGRN PVQLAKSVND IATIYVNATV NWESAEASQK
LGFLESQLPI VKARLEKAEQ ELSAYRQQNG AVDIPAEAEI LLKQASEMET MHIQLKQKYE
EQNQRLAGAH PDMVATKAQI RRIDNKLVGL EKRIKNLPRT QQRMVSLARD VQVNTELYTS
LLNSAQEQRI AAAGSLGNSR IVDFAVTPEK PYWPKPGLLL AIAALLGLSA GSALVFLRHS
MQRHDNYPAL LEYQVGIPLY AAIPHSKKQR RLAKLIDQGK DREPGILVSQ DPLDISVESL
RGLRTTLEAT LANHESKVIM VSSPSPGMGK SFVSTNLAAL LASIKKRVLI IDTDMRNGRL
HETFSVSKQP GLSDLLAGRA TLGDIIISLP DVGVDLIPRG SMVLNPAELL VMGDLEETLE
QLKSFYNHIV IDSPPILGAT DAAIIGKHVD ATFLVVKEGR YTAQELEVSF KRFQQVGVKT
NGFIINDMKE GSSYYPYYGY AYTNPEVNPK NTSMFEAGYQ AVGGWIEKYQ NRNYLDADGS
LEEDDDKVRV
//
