ID V5BUR5_9GAMM Unreviewed; 354 AA.
AC V5BUR5;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=dTDP-glucose 4,6-dehydratase {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
DE EC=4.2.1.46 {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
GN Name=rffG {ECO:0000313|EMBL:ESS71589.1};
GN ORFNames=MGMO_94c00040 {ECO:0000313|EMBL:ESS71589.1};
OS Methyloglobulus morosus KoM1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methyloglobulus.
OX NCBI_TaxID=1116472 {ECO:0000313|EMBL:ESS71589.1, ECO:0000313|Proteomes:UP000017842};
RN [1] {ECO:0000313|EMBL:ESS71589.1, ECO:0000313|Proteomes:UP000017842}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KoM1 {ECO:0000313|EMBL:ESS71589.1,
RC ECO:0000313|Proteomes:UP000017842};
RX PubMed=24356841;
RA Poehlein A., Deutzmann J.S., Daniel R., Simeonova D.D.;
RT "Draft Genome Sequence of the Methanotrophic Gammaproteobacterium
RT Methyloglobulus morosus DSM 22980 Strain KoM1.";
RL Genome Announc. 1:e01078-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC Evidence={ECO:0000256|ARBA:ARBA00001539,
CC ECO:0000256|RuleBase:RU004473};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911,
CC ECO:0000256|RuleBase:RU004473};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily.
CC {ECO:0000256|ARBA:ARBA00008178, ECO:0000256|RuleBase:RU004473}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESS71589.1}.
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DR EMBL; AYLO01000090; ESS71589.1; -; Genomic_DNA.
DR RefSeq; WP_023495310.1; NZ_AYLO01000090.1.
DR AlphaFoldDB; V5BUR5; -.
DR STRING; 1116472.MGMO_94c00040; -.
DR PATRIC; fig|1116472.3.peg.2601; -.
DR eggNOG; COG1088; Bacteria.
DR OrthoDB; 9803010at2; -.
DR Proteomes; UP000017842; Unassembled WGS sequence.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01181; dTDP_gluc_dehyt; 1.
DR PANTHER; PTHR43000:SF7; DTDP-D-GLUCOSE 4,6-DEHYDRATASE; 1.
DR PANTHER; PTHR43000; DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU004473, ECO:0000313|EMBL:ESS71589.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000017842}.
FT DOMAIN 4..326
FT /note="NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF16363"
SQ SEQUENCE 354 AA; 39791 MW; 7220E3E75CA37C97 CRC64;
MKILITGGAG FIGSALIRHV IGNTEHSVVN LDKLTYAGNL ESLKSIEANP RYQFVQADIC
DAVSVNAILQ QHQPDAIMHL AAESHVDRSI DGPAAFIETN VVGTYILVEA ARNYWHALAD
DKKAQFRFHH ISTDEVYGDL PNDGSLFTEQ TPYSPSSPYS ASKASSDHIV RAWHRTYKFP
VVVTNCSNNY GPYHFPEKLI PLVILNALDG KPLPVYGDGK QIRDWLFVDD HARALLTVVE
TGKIGETYNI GGHNEQENIY VVRSICNILE ELHPQKPNGI NHYQDLITFV KDRPGHDLRY
AIDAGKIANE LGWTPKETFE TGLKKTVQWY LDNLEWCRHV QDGSYRRERL GLGE
//
