ID V5C0E4_9GAMM Unreviewed; 563 AA.
AC V5C0E4;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE {ECO:0000256|ARBA:ARBA00013280};
GN Name=nifE {ECO:0000313|EMBL:ESS73524.1};
GN ORFNames=MGMO_18c00320 {ECO:0000313|EMBL:ESS73524.1};
OS Methyloglobulus morosus KoM1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methyloglobulus.
OX NCBI_TaxID=1116472 {ECO:0000313|EMBL:ESS73524.1, ECO:0000313|Proteomes:UP000017842};
RN [1] {ECO:0000313|EMBL:ESS73524.1, ECO:0000313|Proteomes:UP000017842}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KoM1 {ECO:0000313|EMBL:ESS73524.1,
RC ECO:0000313|Proteomes:UP000017842};
RX PubMed=24356841;
RA Poehlein A., Deutzmann J.S., Daniel R., Simeonova D.D.;
RT "Draft Genome Sequence of the Methanotrophic Gammaproteobacterium
RT Methyloglobulus morosus DSM 22980 Strain KoM1.";
RL Genome Announc. 1:e01078-13(2013).
CC -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC prosthetic group of nitrogenase (FeMo cofactor).
CC {ECO:0000256|ARBA:ARBA00003171}.
CC -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005155}.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family.
CC {ECO:0000256|ARBA:ARBA00011002, ECO:0000256|RuleBase:RU004021}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESS73524.1}.
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DR EMBL; AYLO01000018; ESS73524.1; -; Genomic_DNA.
DR RefSeq; WP_023493538.1; NZ_AYLO01000018.1.
DR AlphaFoldDB; V5C0E4; -.
DR STRING; 1116472.MGMO_18c00320; -.
DR PATRIC; fig|1116472.3.peg.645; -.
DR eggNOG; COG1251; Bacteria.
DR eggNOG; COG2710; Bacteria.
DR OrthoDB; 9762718at2; -.
DR UniPathway; UPA00782; -.
DR Proteomes; UP000017842; Unassembled WGS sequence.
DR GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR CDD; cd01968; Nitrogenase_NifE_I; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.50.12380; Nitrogenase MoFe cofactor biosynthesis protein NifE, C-terminal; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005973; NifE.
DR NCBIfam; TIGR01283; nifE; 1.
DR PANTHER; PTHR42956; NITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN NIFE; 1.
DR PANTHER; PTHR42956:SF1; NITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN NIFE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU004021};
KW Reference proteome {ECO:0000313|Proteomes:UP000017842}.
FT DOMAIN 45..444
FT /note="Nitrogenase/oxidoreductase component 1"
FT /evidence="ECO:0000259|Pfam:PF00148"
FT DOMAIN 493..542
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..33
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 563 AA; 61630 MW; 29C794C04962D1DA CRC64;
MSSLSGKIQE VFNEPGCEKN QGKSDKERKK GCTKQLQPGG AAGGCAFDGA KIALQPITDV
AHLVHGPIAC EGNSWDNRGS KSSGSNLWRT GFTTDINETD VVFGGEKRLY KSVKEIIEKY
DPPAVFVYQT CVPAMIGDDI DAVCKAASQK FGKPVIPINS PGFVGPKNLG NKLAGEAILD
HVIGTEEPEY TTPYDINIIG EYNLSGELWQ VKPLLDELGI RILCCISGDA KYHDVASSHR
AKAAMMVCSK SMINIARKME DRYDIPFFEG SFYGIEDSSE ALREISRLLI EKGAPAELME
RTEALIAREE ALAWANIEPY KKRLTGKKVL LITGGVKSWS VVAALQEAGM ELVGTSVKKS
TKEDKERIKE LMGEDAHMID DMSPREMYKM LRDAQADIML SGGRSQFIAL KAKMPWLDIN
QERHHAYMGY VGMVELVKEI DKALYNPVWE QVRKRAPWDE QTWEDVADAA IAAEAAAMAA
DPALARDKRR ATTICNCKNI SRGIIEDAIT EFGLTTAALV SEKTLAGTGC GSCSDKLGKI
IKTIDEWNQP VAGAEPSQTQ HAA
//
